##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AocI ApaI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BclI BfrI BsaBI BsmBI BspLU11I
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DrdI Eco81I EcoO65I
FbaI FseI FspI HincII HindII
HpaI KpnI MamI MfeI MluI
MunI NotI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
RsrII SalI SexAI SfuI SgrAI
SnaBI SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 3162 GAATTC [GA]^AATT[CT]
AgeI 534 ACCGGT A^CCGGT
Alw44I 2228 GTGCAC G^TGCAC
Aor51HI 3706 AGCGCT AGC^GCT
ApaLI 2228 GTGCAC G^TGCAC
ApoI 3162 GAATTC [GA]^AATT[CT]
AvrII 1727 CCTAGG C^CTAGG
BbeI 117 GGCGCC GGCGC^C
BbrPI 3842 CACGTG CAC^GTG
BcgI 1466 CGATCGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 583 AGATCT A^GATCT
BlnI 1727 CCTAGG C^CTAGG
BsaAI 3842 CACGTG [CT]AC^GT[GA]
BsiWI 1227 CGTACG C^GTACG
BsmI 2671 GCATTC GAATGC(1/-1)
BspDI 4316 ATCGAT AT^CGAT
BspHI 324 TCATGA T^CATGA
ClaI 4316 ATCGAT AT^CGAT
DraI 4613 TTTAAA TTT^AAA
EagI 3555 CGGCCG C^GGCCG
EclXI 3555 CGGCCG C^GGCCG
Eco47III 3706 AGCGCT AGC^GCT
Eco52I 3555 CGGCCG C^GGCCG
EcoNI 601 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3162 GAATTC G^AATTC
EcoRV 1613 GATATC GAT^ATC
HindIII 4442 AAGCTT A^AGCTT
KasI 117 GGCGCC G^GCGCC
KspI 3511 CCGCGG CCGC^GG
NaeI 1513 GCCGGC GCC^GGC
NarI 117 GGCGCC GG^CGCC
NdeI 2805 CATATG CA^TATG
NgoMIV 1513 GCCGGC G^CCGGC
NheI 3709 GCTAGC G^CTAGC
NruI 3763 TCGCGA TCG^CGA
PinAI 534 ACCGGT A^CCGGT
PmaCI 3842 CACGTG CAC^GTG
PmlI 3842 CACGTG CAC^GTG
PvuI 1466 CGATCG CGAT^CG
RcaI 324 TCATGA T^CATGA
SacI 1927 GAGCTC GAGCT^C
SacII 3511 CCGCGG CCGC^GG
SapI 816 GCTCTTC GCTCTTC(1/4)
ScaI 694 AGTACT AGT^ACT
SfiI 184 GGCCAGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 117 GGCGCC GGC^GCC
SpeI 2966 ACTAGT A^CTAGT
SspI 4609 AATATT AAT^ATT
StuI 858 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 851 TCCGGA T^CCGGA
879 TCCGGA
AcyI 117 GGCGCC G[GA]^CG[CT]C
3755 GACGCC
AhdI 1251 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2775 GACCCAGCGTC
AspEI 1251 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2775 GACCCAGCGTC
BamHI 1335 GGATCC G^GATCC
3507 GGATCC
BciVI 3154 GGATAC GTATCC(6/5)
1322 GTATCC
BsaHI 117 GGCGCC G[GA]^CG[CT]C
3755 GACGCC
BseAI 851 TCCGGA T^CCGGA
879 TCCGGA
BspEI 851 TCCGGA T^CCGGA
879 TCCGGA
BsrDI 2038 GCAATG GCAATG(2/0)
4602 GCAATG
BssHII 3673 GCGCGC G^CGCGC
3878 GCGCGC
BstAPI 2251 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2260 GCAACAGATGC
BstXI 2952 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3294 CCACCCAGGTGG
DraIII 72 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2157 CACTAGGTG
Eam1105I 1251 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2775 GACCCAGCGTC
HgaI 3755 GACGC GACGC(5/10)
2781 GCGTC
Hin1I 117 GGCGCC G[GA]^CG[CT]C
3755 GACGCC
MaeII 893 ACGT A^CGT
3843 ACGT
MroI 851 TCCGGA T^CCGGA
879 TCCGGA
MseI 510 TTAA T^TAA
4614 TTAA
NcoI 1741 CCATGG C^CATGG
2143 CCATGG
PflMI 71 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
160 CCAGGCAGTGG
SbfI 700 CCTGCAGG CCTGCA^GG
2383 CCTGCAGG
Sse8387I 700 CCTGCAGG CCTGCA^GG
2383 CCTGCAGG
TaqI 429 TCGA T^CGA
4317 TCGA
Tru9I 510 TTAA T^TAA
4614 TTAA
TthHB8I 429 TCGA T^CGA
4317 TCGA
Van91I 71 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
160 CCAGGCAGTGG
XbaI 838 TCTAGA T^CTAGA
1234 TCTAGA
XcmI 460 CCATGCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2152 CCAGACACTAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrFI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StyI TfiI TseI Tsp45I Tsp509I
TspRI XhoII XmaCI XmaI