##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

Acc65I      AccII       AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BbrPI       BcgI        BfrI        BlnI        BsaBI       
BspDI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstZ17I     ClaI        CpoI        DrdI        
Eam1105I    EcoRV       FseI        FspI        HgaI        
HindIII     KpnI        KspI        MamI        MfeI        
MluI        MunI        MvnI        NaeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SnaBI       Sse8387I    StuI        SwaI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AatII        1581  GACGTC               GACGT^C
AccIII       2750  TCCGGA               T^CCGGA
AocI          224  CCTAAGG              CC^T[ACGT]AGG
Aor51HI       557  AGCGCT               AGC^GCT
ApaI         1338  GGGCCC               GGGCC^C
Asp700I       134  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         4295  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI         3564  GGCGCC               GGCGC^C
BbsI         4108  GTCTTC               GAAGAC(2/6)
BciVI        2312  GTATCC               GTATCC(6/5)
BclI         3148  TGATCA               T^GATCA
BlpI         1755  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1755  GCTGAGC              GC^T[ACGT]AGC
BpuAI        4108  GTCTTC               GAAGAC(2/6)
BsaAI        1846  TACGTG               [CT]AC^GT[GA]
BsaWI        2750  TCCGGA               [AT]^CCGG[AT]
BseAI        2750  TCCGGA               T^CCGGA
BsiEI        1665  CGGCCG               CG[GA][CT]^CG
BsiWI        1844  CGTACG               C^GTACG
BsmBI         663  GAGACG               CGTCTC(1/5)
Bsp1407I     2703  TGTACA               T^GTACA
BspEI        2750  TCCGGA               T^CCGGA
BspHI        3719  TCATGA               T^CATGA
BspLU11I     4151  ACATGT               A^CATGT
BsrGI        2703  TGTACA               T^GTACA
BstAPI       2400  GCAAGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        224  CCTAAGG              CC^T[ACGT]AGG
CelII        1755  GCTGAGC              GC^T[ACGT]AGC
DraI         4317  TTTAAA               TTT^AAA
EagI         1665  CGGCCG               C^GGCCG
EclXI        1665  CGGCCG               C^GGCCG
Eco47III      557  AGCGCT               AGC^GCT
Eco52I       1665  CGGCCG               C^GGCCG
Eco81I        224  CCTAAGG              CC^T[ACGT]AGG
EcoRI        2636  GAATTC               G^AATTC
EcoT22I      1039  ATGCAT               ATGCA^T
FauI         2117  GCGGG                CCCGC(4/6)
FbaI         3148  TGATCA               T^GATCA
KasI         3564  GGCGCC               G^GCGCC
MroI         2750  TCCGGA               T^CCGGA
NarI         3564  GGCGCC               GG^CGCC
NcoI         1183  CCATGG               C^CATGG
NsiI         1039  ATGCAT               ATGCA^T
PaeR7I       1408  CTCGAG               C^TCGAG
PflFI        4295  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1178  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         220  GGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         3719  TCATGA               T^CATGA
SfoI         3564  GGCGCC               GGC^GCC
SpeI           20  ACTAGT               A^CTAGT
SphI         3354  GCATGC               GCATG^C
SspBI        2703  TGTACA               T^GTACA
SspI         3771  AATATT               AAT^ATT
Tth111I      4295  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1178  CCAGGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1408  CTCGAG               C^TCGAG
XmnI          134  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI         2636  GAATTC               [GA]^AATT[CT]
             4306  AAATTT              
AcyI         1581  GACGTC               G[GA]^CG[CT]C
             3564  GGCGCC              
AflIII       1847  ACGTGT               A^C[GA][CT]GT
             4151  ACATGT              
ApoI         2636  GAATTC               [GA]^AATT[CT]
             4306  AAATTT              
BalI          241  TGGCCA               TGG^CCA
             3121  TGGCCA              
BamHI        1033  GGATCC               G^GATCC
             2753  GGATCC              
BanI         3159  GGTGCC               G^G[CT][GA]CC
             3564  GGCGCC              
BglI          570  GCCTGTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1156  GCCACACAGGC         
BglII        1508  AGATCT               A^GATCT
             3971  AGATCT              
BsaHI        1581  GACGTC               G[GA]^CG[CT]C
             3564  GGCGCC              
BsrBI        1329  GAGCGG               CCGCTC(-3/-3)
             2115  GAGCGG              
BsrFI         802  ACCGGC               [GA]^CCGG[CT]
             3056  ACCGGC              
BstEII        485  GGTCACC              G^GT[ACGT]ACC
             1616  GGTGACC             
BstPI         485  GGTCACC              G^GT[ACGT]ACC
             1616  GGTGACC             
Cfr10I        802  ACCGGC               [GA]^CCGG[CT]
             3056  ACCGGC              
DraIII        601  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1294  CACGCTGTG           
EcoNI        1247  CCTCTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1277  CCTACTGCAGG         
EcoO65I       485  GGTCACC              G^GT[ACGT]ACC
             1616  GGTGACC             
Hin1I        1581  GACGTC               G[GA]^CG[CT]C
             3564  GGCGCC              
HincII       3448  GTTAAC               GT[CT]^[GA]AC
             3500  GTTAAC              
HindII       3448  GTTAAC               GT[CT]^[GA]AC
             3500  GTTAAC              
HpaI         3448  GTTAAC               GTT^AAC
             3500  GTTAAC              
MluNI         241  TGGCCA               TGG^CCA
             3121  TGGCCA              
MscI          241  TGGCCA               TGG^CCA
             3121  TGGCCA              
MslI          750  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             3082  CATCTGAGTG          
NdeI          700  CATATG               CA^TATG
             4052  CATATG              
SacI         2549  GAGCTC               GAGCT^C
             2560  GAGCTC              
SexAI         469  ACCTGGT              A^CC[AT]GGT
             1391  ACCTGGT             
XbaI          863  TCTAGA               T^CTAGA
              977  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccI        AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanII       
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII       
XmaCI       XmaI