##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbrPI BcgI BfrI BlnI BsaBI
BspDI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I ClaI CpoI DrdI
Eam1105I EcoRV FseI FspI HgaI
HindIII KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI Sse8387I StuI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1581 GACGTC GACGT^C
AccIII 2750 TCCGGA T^CCGGA
AocI 224 CCTAAGG CC^T[ACGT]AGG
Aor51HI 557 AGCGCT AGC^GCT
ApaI 1338 GGGCCC GGGCC^C
Asp700I 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4295 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3564 GGCGCC GGCGC^C
BbsI 4108 GTCTTC GAAGAC(2/6)
BciVI 2312 GTATCC GTATCC(6/5)
BclI 3148 TGATCA T^GATCA
BlpI 1755 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1755 GCTGAGC GC^T[ACGT]AGC
BpuAI 4108 GTCTTC GAAGAC(2/6)
BsaAI 1846 TACGTG [CT]AC^GT[GA]
BsaWI 2750 TCCGGA [AT]^CCGG[AT]
BseAI 2750 TCCGGA T^CCGGA
BsiEI 1665 CGGCCG CG[GA][CT]^CG
BsiWI 1844 CGTACG C^GTACG
BsmBI 663 GAGACG CGTCTC(1/5)
Bsp1407I 2703 TGTACA T^GTACA
BspEI 2750 TCCGGA T^CCGGA
BspHI 3719 TCATGA T^CATGA
BspLU11I 4151 ACATGT A^CATGT
BsrGI 2703 TGTACA T^GTACA
BstAPI 2400 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 224 CCTAAGG CC^T[ACGT]AGG
CelII 1755 GCTGAGC GC^T[ACGT]AGC
DraI 4317 TTTAAA TTT^AAA
EagI 1665 CGGCCG C^GGCCG
EclXI 1665 CGGCCG C^GGCCG
Eco47III 557 AGCGCT AGC^GCT
Eco52I 1665 CGGCCG C^GGCCG
Eco81I 224 CCTAAGG CC^T[ACGT]AGG
EcoRI 2636 GAATTC G^AATTC
EcoT22I 1039 ATGCAT ATGCA^T
FauI 2117 GCGGG CCCGC(4/6)
FbaI 3148 TGATCA T^GATCA
KasI 3564 GGCGCC G^GCGCC
MroI 2750 TCCGGA T^CCGGA
NarI 3564 GGCGCC GG^CGCC
NcoI 1183 CCATGG C^CATGG
NsiI 1039 ATGCAT ATGCA^T
PaeR7I 1408 CTCGAG C^TCGAG
PflFI 4295 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1178 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 220 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 3719 TCATGA T^CATGA
SfoI 3564 GGCGCC GGC^GCC
SpeI 20 ACTAGT A^CTAGT
SphI 3354 GCATGC GCATG^C
SspBI 2703 TGTACA T^GTACA
SspI 3771 AATATT AAT^ATT
Tth111I 4295 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1178 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1408 CTCGAG C^TCGAG
XmnI 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 2636 GAATTC [GA]^AATT[CT]
4306 AAATTT
AcyI 1581 GACGTC G[GA]^CG[CT]C
3564 GGCGCC
AflIII 1847 ACGTGT A^C[GA][CT]GT
4151 ACATGT
ApoI 2636 GAATTC [GA]^AATT[CT]
4306 AAATTT
BalI 241 TGGCCA TGG^CCA
3121 TGGCCA
BamHI 1033 GGATCC G^GATCC
2753 GGATCC
BanI 3159 GGTGCC G^G[CT][GA]CC
3564 GGCGCC
BglI 570 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1156 GCCACACAGGC
BglII 1508 AGATCT A^GATCT
3971 AGATCT
BsaHI 1581 GACGTC G[GA]^CG[CT]C
3564 GGCGCC
BsrBI 1329 GAGCGG CCGCTC(-3/-3)
2115 GAGCGG
BsrFI 802 ACCGGC [GA]^CCGG[CT]
3056 ACCGGC
BstEII 485 GGTCACC G^GT[ACGT]ACC
1616 GGTGACC
BstPI 485 GGTCACC G^GT[ACGT]ACC
1616 GGTGACC
Cfr10I 802 ACCGGC [GA]^CCGG[CT]
3056 ACCGGC
DraIII 601 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1294 CACGCTGTG
EcoNI 1247 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1277 CCTACTGCAGG
EcoO65I 485 GGTCACC G^GT[ACGT]ACC
1616 GGTGACC
Hin1I 1581 GACGTC G[GA]^CG[CT]C
3564 GGCGCC
HincII 3448 GTTAAC GT[CT]^[GA]AC
3500 GTTAAC
HindII 3448 GTTAAC GT[CT]^[GA]AC
3500 GTTAAC
HpaI 3448 GTTAAC GTT^AAC
3500 GTTAAC
MluNI 241 TGGCCA TGG^CCA
3121 TGGCCA
MscI 241 TGGCCA TGG^CCA
3121 TGGCCA
MslI 750 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3082 CATCTGAGTG
NdeI 700 CATATG CA^TATG
4052 CATATG
SacI 2549 GAGCTC GAGCT^C
2560 GAGCTC
SexAI 469 ACCTGGT A^CC[AT]GGT
1391 ACCTGGT
XbaI 863 TCTAGA T^CTAGA
977 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI