################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AccII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BcgI BfrI BlnI BsaBI BspDI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI DrdI Eam1105I EcoRV FseI FspI HgaI HindIII KpnI KspI MamI MfeI MluI MunI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 1581 GACGTC GACGT^C AccIII 2750 TCCGGA T^CCGGA AocI 224 CCTAAGG CC^T[ACGT]AGG Aor51HI 557 AGCGCT AGC^GCT ApaI 1338 GGGCCC GGGCC^C Asp700I 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 4295 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 3564 GGCGCC GGCGC^C BbsI 4108 GTCTTC GAAGAC(2/6) BciVI 2312 GTATCC GTATCC(6/5) BclI 3148 TGATCA T^GATCA BlpI 1755 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1755 GCTGAGC GC^T[ACGT]AGC BpuAI 4108 GTCTTC GAAGAC(2/6) BsaAI 1846 TACGTG [CT]AC^GT[GA] BsaWI 2750 TCCGGA [AT]^CCGG[AT] BseAI 2750 TCCGGA T^CCGGA BsiEI 1665 CGGCCG CG[GA][CT]^CG BsiWI 1844 CGTACG C^GTACG BsmBI 663 GAGACG CGTCTC(1/5) Bsp1407I 2703 TGTACA T^GTACA BspEI 2750 TCCGGA T^CCGGA BspHI 3719 TCATGA T^CATGA BspLU11I 4151 ACATGT A^CATGT BsrGI 2703 TGTACA T^GTACA BstAPI 2400 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 224 CCTAAGG CC^T[ACGT]AGG CelII 1755 GCTGAGC GC^T[ACGT]AGC DraI 4317 TTTAAA TTT^AAA EagI 1665 CGGCCG C^GGCCG EclXI 1665 CGGCCG C^GGCCG Eco47III 557 AGCGCT AGC^GCT Eco52I 1665 CGGCCG C^GGCCG Eco81I 224 CCTAAGG CC^T[ACGT]AGG EcoRI 2636 GAATTC G^AATTC EcoT22I 1039 ATGCAT ATGCA^T FauI 2117 GCGGG CCCGC(4/6) FbaI 3148 TGATCA T^GATCA KasI 3564 GGCGCC G^GCGCC MroI 2750 TCCGGA T^CCGGA NarI 3564 GGCGCC GG^CGCC NcoI 1183 CCATGG C^CATGG NsiI 1039 ATGCAT ATGCA^T PaeR7I 1408 CTCGAG C^TCGAG PflFI 4295 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1178 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 220 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 3719 TCATGA T^CATGA SfoI 3564 GGCGCC GGC^GCC SpeI 20 ACTAGT A^CTAGT SphI 3354 GCATGC GCATG^C SspBI 2703 TGTACA T^GTACA SspI 3771 AATATT AAT^ATT Tth111I 4295 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1178 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1408 CTCGAG C^TCGAG XmnI 134 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 2636 GAATTC [GA]^AATT[CT] 4306 AAATTT AcyI 1581 GACGTC G[GA]^CG[CT]C 3564 GGCGCC AflIII 1847 ACGTGT A^C[GA][CT]GT 4151 ACATGT ApoI 2636 GAATTC [GA]^AATT[CT] 4306 AAATTT BalI 241 TGGCCA TGG^CCA 3121 TGGCCA BamHI 1033 GGATCC G^GATCC 2753 GGATCC BanI 3159 GGTGCC G^G[CT][GA]CC 3564 GGCGCC BglI 570 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1156 GCCACACAGGC BglII 1508 AGATCT A^GATCT 3971 AGATCT BsaHI 1581 GACGTC G[GA]^CG[CT]C 3564 GGCGCC BsrBI 1329 GAGCGG CCGCTC(-3/-3) 2115 GAGCGG BsrFI 802 ACCGGC [GA]^CCGG[CT] 3056 ACCGGC BstEII 485 GGTCACC G^GT[ACGT]ACC 1616 GGTGACC BstPI 485 GGTCACC G^GT[ACGT]ACC 1616 GGTGACC Cfr10I 802 ACCGGC [GA]^CCGG[CT] 3056 ACCGGC DraIII 601 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1294 CACGCTGTG EcoNI 1247 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1277 CCTACTGCAGG EcoO65I 485 GGTCACC G^GT[ACGT]ACC 1616 GGTGACC Hin1I 1581 GACGTC G[GA]^CG[CT]C 3564 GGCGCC HincII 3448 GTTAAC GT[CT]^[GA]AC 3500 GTTAAC HindII 3448 GTTAAC GT[CT]^[GA]AC 3500 GTTAAC HpaI 3448 GTTAAC GTT^AAC 3500 GTTAAC MluNI 241 TGGCCA TGG^CCA 3121 TGGCCA MscI 241 TGGCCA TGG^CCA 3121 TGGCCA MslI 750 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3082 CATCTGAGTG NdeI 700 CATATG CA^TATG 4052 CATATG SacI 2549 GAGCTC GAGCT^C 2560 GAGCTC SexAI 469 ACCTGGT A^CC[AT]GGT 1391 ACCTGGT XbaI 863 TCTAGA T^CTAGA 977 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI