##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AocI Aor51HI AscI AspEI AspI
AviII AvrII BalI BbrPI BcgI
BciVI BlnI BseAI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI Bst1107I
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoT22I FseI FspI HincII
HindII HindIII HpaI MluI MluNI
MroI MscI NaeI NgoMIV NotI
NruI NsiI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SfiI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2506 GGTACC G^GTACC
AccI 264 GTCTAC GT^[AC][GT]AC
AflIII 4590 ACGTGT A^C[GA][CT]GT
Alw44I 3927 GTGCAC G^TGCAC
AlwNI 2546 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3927 GTGCAC G^TGCAC
AseI 3709 ATTAAT AT^TAAT
AsnI 3709 ATTAAT AT^TAAT
Asp718I 2506 GGTACC G^GTACC
BbeI 114 GGCGCC GGCGC^C
BclI 503 TGATCA T^GATCA
BglI 1532 GCCATATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3481 CACGTA [CT]AC^GT[GA]
BsaBI 1900 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3509 TCCGGT [AT]^CCGG[AT]
BsiEI 128 CGGCCG CG[GA][CT]^CG
BsiWI 3483 CGTACG C^GTACG
BsmI 410 GAATGC GAATGC(1/-1)
BspMI 1134 ACCTGC ACCTGC(4/8)
BssHII 56 GCGCGC G^CGCGC
BstAPI 609 GCATACCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1146 TTCGAA TT^CGAA
DraIII 2686 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 25 GACCGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 128 CGGCCG C^GGCCG
EclXI 128 CGGCCG C^GGCCG
Eco52I 128 CGGCCG C^GGCCG
EcoNI 1522 CCTGTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2026 GAATTC G^AATTC
FbaI 503 TGATCA T^GATCA
HaeII 114 GGCGCC [GA]GCGC^[CT]
KasI 114 GGCGCC G^GCGCC
KpnI 2506 GGTACC GGTAC^C
MamI 1900 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 350 CAATTG C^AATTG
MunI 350 CAATTG C^AATTG
NarI 114 GGCGCC GG^CGCC
NcoI 698 CCATGG C^CATGG
NheI 4491 GCTAGC G^CTAGC
NspV 1146 TTCGAA TT^CGAA
PacI 4447 TTAATTAA TTAAT^TAA
PflMI 3412 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2771 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 989 GACGAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3709 ATTAAT AT^TAAT
ScaI 4549 AGTACT AGT^ACT
SexAI 1833 ACCAGGT A^CC[AT]GGT
SfoI 114 GGCGCC GGC^GCC
SfuI 1146 TTCGAA TT^CGAA
SphI 3247 GCATGC GCATG^C
StuI 4309 AGGCCT AGG^CCT
Van91I 3412 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 114 GGCGCC G[GA]^CG[CT]C
3610 GACGCC
AflII 2808 CTTAAG C^TTAAG
3436 CTTAAG
ApaI 1587 GGGCCC GGGCC^C
3167 GGGCCC
Asp700I 1352 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4665 GAACTTTTTC
AvaI 1252 CCCGAG C^[CT]CG[GA]G
2618 CCCGAG
BamHI 39 GGATCC G^GATCC
1419 GGATCC
BfrI 2808 CTTAAG C^TTAAG
3436 CTTAAG
BglII 564 AGATCT A^GATCT
4169 AGATCT
BlpI 1080 GCTCAGC GC^T[ACGT]AGC
1978 GCTCAGC
Bpu1102I 1080 GCTCAGC GC^T[ACGT]AGC
1978 GCTCAGC
BsaHI 114 GGCGCC G[GA]^CG[CT]C
3610 GACGCC
BsgI 1951 GTGCAG GTGCAG(16/14)
2764 GTGCAG
BsoBI 1252 CCCGAG C^[CT]CG[GA]G
2618 CCCGAG
CelII 1080 GCTCAGC GC^T[ACGT]AGC
1978 GCTCAGC
DraI 435 TTTAAA TTT^AAA
4195 TTTAAA
EaeI 8 CGGCCA [CT]^GGCC[GA]
128 CGGCCG
EcoRV 253 GATATC GAT^ATC
703 GATATC
EcoT14I 698 CCATGG C^C[AT][AT]GG
1469 CCAAGG
Hin1I 114 GGCGCC G[GA]^CG[CT]C
3610 GACGCC
MslI 1014 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3349 CATGGTGATG
NdeI 579 CATATG CA^TATG
2093 CATATG
PstI 1075 CTGCAG CTGCA^G
2699 CTGCAG
SpeI 837 ACTAGT A^CTAGT
4081 ACTAGT
StyI 698 CCATGG C^C[AT][AT]GG
1469 CCAAGG
XmnI 1352 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4665 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII