################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AclI AgeI Aor51HI AscI AviII AvrII BamHI BbeI BbrPI BlnI BsaBI BsiEI BsiWI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Cfr10I CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HaeII HpaI KasI KspI MamI MluI NaeI NarI NgoMIV NotI NruI PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 4002 GGTACC G^GTACC AccI 691 GTCTAC GT^[AC][GT]AC AccII 152 CGCG CG^CG AccIII 3674 TCCGGA T^CCGGA AcyI 637 GACGCC G[GA]^CG[CT]C AflII 5072 CTTAAG C^TTAAG AhdI 637 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1974 GTGCAC G^TGCAC ApaI 487 GGGCCC GGGCC^C ApaLI 1974 GTGCAC G^TGCAC Asp718I 4002 GGTACC G^GTACC AspEI 637 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3307 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 879 TGGCCA TGG^CCA BfrI 5072 CTTAAG C^TTAAG BglII 4554 AGATCT A^GATCT BlpI 979 GCTCAGC GC^T[ACGT]AGC Bpu1102I 979 GCTCAGC GC^T[ACGT]AGC BsaAI 3577 TACGTG [CT]AC^GT[GA] BsaHI 637 GACGCC G[GA]^CG[CT]C BseAI 3674 TCCGGA T^CCGGA BspDI 2308 ATCGAT AT^CGAT BspEI 3674 TCCGGA T^CCGGA BspHI 713 TCATGA T^CATGA BspLU11I 3830 ACATGT A^CATGT BsrBI 131 CCGCTC CCGCTC(-3/-3) BstAPI 2706 GCAAATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1676 TTCGAA TT^CGAA BstUI 152 CGCG CG^CG CelII 979 GCTCAGC GC^T[ACGT]AGC ClaI 2308 ATCGAT AT^CGAT EaeI 879 TGGCCA [CT]^GGCC[GA] Eam1105I 637 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 541 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2302 GATATC GAT^ATC EcoT22I 1079 ATGCAT ATGCA^T Hin1I 637 GACGCC G[GA]^CG[CT]C HincII 3683 GTCAAC GT[CT]^[GA]AC HindII 3683 GTCAAC GT[CT]^[GA]AC KpnI 4002 GGTACC GGTAC^C MfeI 2937 CAATTG C^AATTG MluNI 879 TGGCCA TGG^CCA MroI 3674 TCCGGA T^CCGGA MscI 879 TGGCCA TGG^CCA MunI 2937 CAATTG C^AATTG MvnI 152 CGCG CG^CG NdeI 1076 CATATG CA^TATG NheI 4867 GCTAGC G^CTAGC NsiI 1079 ATGCAT ATGCA^T NspV 1676 TTCGAA TT^CGAA PaeR7I 3991 CTCGAG C^TCGAG PflFI 3307 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3965 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 359 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 713 TCATGA T^CATGA SapI 1684 GCTCTTC GCTCTTC(1/4) SfuI 1676 TTCGAA TT^CGAA SpeI 4728 ACTAGT A^CTAGT SwaI 723 ATTTAAAT ATTT^AAAT Tth111I 3307 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3965 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2213 CCAAATTTCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3991 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 3562 ACACGT A^C[GA][CT]GT 3830 ACATGT AlwI 1542 GATCC GGATC(4/5) 2454 GGATC AseI 3264 ATTAAT AT^TAAT 4596 ATTAAT AsnI 3264 ATTAAT AT^TAAT 4596 ATTAAT BcgI 4259 CGAAAGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3936 GCATGTGCCTCG BclI 797 TGATCA T^GATCA 3812 TGATCA BglI 1342 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1512 GCCACAGAGGC BsaWI 3674 TCCGGA [AT]^CCGG[AT] 3836 ACCGGA BsmBI 3103 CGTCTC CGTCTC(1/5) 7 GAGACG BstXI 537 CCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4438 CCATTTGGATGG BstYI 1541 AGATCC [GA]^GATC[CT] 4554 AGATCT CfoI 111 GCGC GCG^C 153 GCGC DraIII 2653 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 4039 CACTTTGTG FbaI 797 TGATCA T^GATCA 3812 TGATCA HhaI 111 GCGC GCG^C 153 GCGC HinP1I 111 GCGC G^CGC 153 GCGC HindIII 1860 AAGCTT A^AGCTT 5062 AAGCTT MflI 1541 AGATCC [GA]^GATC[CT] 4554 AGATCT PshBI 3264 ATTAAT AT^TAAT 4596 ATTAAT PstI 667 CTGCAG CTGCA^G 4099 CTGCAG SexAI 449 ACCAGGT A^CC[AT]GGT 656 ACCAGGT XhoII 1541 AGATCC [GA]^GATC[CT] 4554 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI