##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI Aor51HI AscI
AviII AvrII BamHI BbeI BbrPI
BlnI BsaBI BsiEI BsiWI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Cfr10I CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI HaeII HpaI KasI KspI
MamI MluI NaeI NarI NgoMIV
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 4002 GGTACC G^GTACC
AccI 691 GTCTAC GT^[AC][GT]AC
AccII 152 CGCG CG^CG
AccIII 3674 TCCGGA T^CCGGA
AcyI 637 GACGCC G[GA]^CG[CT]C
AflII 5072 CTTAAG C^TTAAG
AhdI 637 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1974 GTGCAC G^TGCAC
ApaI 487 GGGCCC GGGCC^C
ApaLI 1974 GTGCAC G^TGCAC
Asp718I 4002 GGTACC G^GTACC
AspEI 637 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3307 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 879 TGGCCA TGG^CCA
BfrI 5072 CTTAAG C^TTAAG
BglII 4554 AGATCT A^GATCT
BlpI 979 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 979 GCTCAGC GC^T[ACGT]AGC
BsaAI 3577 TACGTG [CT]AC^GT[GA]
BsaHI 637 GACGCC G[GA]^CG[CT]C
BseAI 3674 TCCGGA T^CCGGA
BspDI 2308 ATCGAT AT^CGAT
BspEI 3674 TCCGGA T^CCGGA
BspHI 713 TCATGA T^CATGA
BspLU11I 3830 ACATGT A^CATGT
BsrBI 131 CCGCTC CCGCTC(-3/-3)
BstAPI 2706 GCAAATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1676 TTCGAA TT^CGAA
BstUI 152 CGCG CG^CG
CelII 979 GCTCAGC GC^T[ACGT]AGC
ClaI 2308 ATCGAT AT^CGAT
EaeI 879 TGGCCA [CT]^GGCC[GA]
Eam1105I 637 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 541 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2302 GATATC GAT^ATC
EcoT22I 1079 ATGCAT ATGCA^T
Hin1I 637 GACGCC G[GA]^CG[CT]C
HincII 3683 GTCAAC GT[CT]^[GA]AC
HindII 3683 GTCAAC GT[CT]^[GA]AC
KpnI 4002 GGTACC GGTAC^C
MfeI 2937 CAATTG C^AATTG
MluNI 879 TGGCCA TGG^CCA
MroI 3674 TCCGGA T^CCGGA
MscI 879 TGGCCA TGG^CCA
MunI 2937 CAATTG C^AATTG
MvnI 152 CGCG CG^CG
NdeI 1076 CATATG CA^TATG
NheI 4867 GCTAGC G^CTAGC
NsiI 1079 ATGCAT ATGCA^T
NspV 1676 TTCGAA TT^CGAA
PaeR7I 3991 CTCGAG C^TCGAG
PflFI 3307 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3965 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 359 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 713 TCATGA T^CATGA
SapI 1684 GCTCTTC GCTCTTC(1/4)
SfuI 1676 TTCGAA TT^CGAA
SpeI 4728 ACTAGT A^CTAGT
SwaI 723 ATTTAAAT ATTT^AAAT
Tth111I 3307 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3965 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2213 CCAAATTTCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3991 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 3562 ACACGT A^C[GA][CT]GT
3830 ACATGT
AlwI 1542 GATCC GGATC(4/5)
2454 GGATC
AseI 3264 ATTAAT AT^TAAT
4596 ATTAAT
AsnI 3264 ATTAAT AT^TAAT
4596 ATTAAT
BcgI 4259 CGAAAGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3936 GCATGTGCCTCG
BclI 797 TGATCA T^GATCA
3812 TGATCA
BglI 1342 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1512 GCCACAGAGGC
BsaWI 3674 TCCGGA [AT]^CCGG[AT]
3836 ACCGGA
BsmBI 3103 CGTCTC CGTCTC(1/5)
7 GAGACG
BstXI 537 CCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4438 CCATTTGGATGG
BstYI 1541 AGATCC [GA]^GATC[CT]
4554 AGATCT
CfoI 111 GCGC GCG^C
153 GCGC
DraIII 2653 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
4039 CACTTTGTG
FbaI 797 TGATCA T^GATCA
3812 TGATCA
HhaI 111 GCGC GCG^C
153 GCGC
HinP1I 111 GCGC G^CGC
153 GCGC
HindIII 1860 AAGCTT A^AGCTT
5062 AAGCTT
MflI 1541 AGATCC [GA]^GATC[CT]
4554 AGATCT
PshBI 3264 ATTAAT AT^TAAT
4596 ATTAAT
PstI 667 CTGCAG CTGCA^G
4099 CTGCAG
SexAI 449 ACCAGGT A^CC[AT]GGT
656 ACCAGGT
XhoII 1541 AGATCC [GA]^GATC[CT]
4554 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
AocI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI