##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AvrII BbeI BclI BfrI
BlnI BsiWI BsmBI BsmI BspDI
BsrDI BssHII BssSI BstAPI BstBI
ClaI CpoI Eam1105I EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
KspI MluI NarI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 4687 CGCG CG^CG
AccIII 352 TCCGGA T^CCGGA
AcyI 752 GACGCC G[GA]^CG[CT]C
Alw44I 858 GTGCAC G^TGCAC
Aor51HI 424 AGCGCT AGC^GCT
ApaLI 858 GTGCAC G^TGCAC
AspI 564 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1030 TGCGCA TGC^GCA
BcgI 170 CGACGTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2458 GTATCC GTATCC(6/5)
BsaBI 134 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 752 GACGCC G[GA]^CG[CT]C
BsaWI 352 TCCGGA [AT]^CCGG[AT]
BseAI 352 TCCGGA T^CCGGA
BsgI 999 GTGCAG GTGCAG(16/14)
Bsp1407I 4208 TGTACA T^GTACA
BspEI 352 TCCGGA T^CCGGA
BsrBI 9 GAGCGG CCGCTC(-3/-3)
BsrGI 4208 TGTACA T^GTACA
Bst1107I 3478 GTATAC GTA^TAC
BstEII 1080 GGTCACC G^GT[ACGT]ACC
BstPI 1080 GGTCACC G^GT[ACGT]ACC
BstUI 4687 CGCG CG^CG
BstZ17I 3478 GTATAC GTA^TAC
DraIII 3294 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3961 GACAAGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 424 AGCGCT AGC^GCT
EcoO65I 1080 GGTCACC G^GT[ACGT]ACC
EcoRV 2448 GATATC GAT^ATC
FspI 1030 TGCGCA TGC^GCA
HaeII 424 AGCGCT [GA]GCGC^[CT]
Hin1I 752 GACGCC G[GA]^CG[CT]C
HincII 4221 GTTGAC GT[CT]^[GA]AC
HindII 4221 GTTGAC GT[CT]^[GA]AC
MamI 134 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4228 CAATTG C^AATTG
MroI 352 TCCGGA T^CCGGA
MunI 4228 CAATTG C^AATTG
MvnI 4687 CGCG CG^CG
NaeI 604 GCCGGC GCC^GGC
NgoMIV 604 GCCGGC G^CCGGC
NheI 1835 GCTAGC G^CTAGC
PaeR7I 2634 CTCGAG C^TCGAG
PflFI 564 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 769 AGTACT AGT^ACT
SmaI 3806 CCCGGG CCC^GGG
SphI 3425 GCATGC GCATG^C
SspBI 4208 TGTACA T^GTACA
Tth111I 564 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2634 CTCGAG C^TCGAG
XmaCI 3806 CCCGGG C^CCGGG
XmaI 3806 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1212 GTCTAC GT^[AC][GT]AC
3478 GTATAC
AcsI 2621 GAATTC [GA]^AATT[CT]
4548 GAATTC
AocI 1863 CCTTAGG CC^T[ACGT]AGG
2894 CCTTAGG
ApoI 2621 GAATTC [GA]^AATT[CT]
4548 GAATTC
BalI 1244 TGGCCA TGG^CCA
3910 TGGCCA
BamHI 977 GGATCC G^GATCC
1663 GGATCC
BbrPI 521 CACGTG CAC^GTG
1314 CACGTG
BsaAI 521 CACGTG [CT]AC^GT[GA]
1314 CACGTG
BsaI 3348 GAGACC GGTCTC(1/5)
3255 GGTCTC
BspHI 2004 TCATGA T^CATGA
4013 TCATGA
BsrFI 604 GCCGGC [GA]^CCGG[CT]
944 GCCGGT
Bsu36I 1863 CCTTAGG CC^T[ACGT]AGG
2894 CCTTAGG
Cfr10I 604 GCCGGC [GA]^CCGG[CT]
944 GCCGGT
DraI 3697 TTTAAA TTT^AAA
4751 TTTAAA
EagI 298 CGGCCG C^GGCCG
1153 CGGCCG
EclXI 298 CGGCCG C^GGCCG
1153 CGGCCG
Eco52I 298 CGGCCG C^GGCCG
1153 CGGCCG
Eco81I 1863 CCTTAGG CC^T[ACGT]AGG
2894 CCTTAGG
EcoRI 2621 GAATTC G^AATTC
4548 GAATTC
HgaI 564 GACGC GACGC(5/10)
752 GACGC
MluNI 1244 TGGCCA TGG^CCA
3910 TGGCCA
MscI 1244 TGGCCA TGG^CCA
3910 TGGCCA
PmaCI 521 CACGTG CAC^GTG
1314 CACGTG
PmlI 521 CACGTG CAC^GTG
1314 CACGTG
RcaI 2004 TCATGA T^CATGA
4013 TCATGA
StuI 2608 AGGCCT AGG^CCT
4456 AGGCCT
XbaI 2669 TCTAGA T^CTAGA
4372 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI Asp700I AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI