##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AccII AclI AflII AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspI AviII BalI BbeI BbrPI
BcgI BcnI BfrI BsiEI BsiWI
BsmBI BspDI BsrBI BssHII BssSI
BstBI BstEII BstPI BstUI ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV FseI
FspI HaeII KasI KspI MluI
MluNI MscI MvnI NaeI NarI
NciI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacII SalI SapI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 2511 GACGTC GACGT^C
Acc65I 3436 GGTACC G^GTACC
AccIII 308 TCCGGA T^CCGGA
AgeI 801 ACCGGT A^CCGGT
AseI 3997 ATTAAT AT^TAAT
AsnI 3997 ATTAAT AT^TAAT
Asp718I 3436 GGTACC G^GTACC
AvaI 137 CTCGGG C^[CT]CG[GA]G
AvrII 2730 CCTAGG C^CTAGG
BanI 3436 GGTACC G^G[CT][GA]CC
BglI 4580 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2730 CCTAGG C^CTAGG
BlpI 501 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 501 GCTCAGC GC^T[ACGT]AGC
BsaAI 5118 TACGTG [CT]AC^GT[GA]
BsaBI 88 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1249 GAGACC GGTCTC(1/5)
BseAI 308 TCCGGA T^CCGGA
BsoBI 137 CTCGGG C^[CT]CG[GA]G
Bsp1407I 5238 TGTACA T^GTACA
BspEI 308 TCCGGA T^CCGGA
BsrFI 801 ACCGGT [GA]^CCGG[CT]
BsrGI 5238 TGTACA T^GTACA
BstXI 3674 CCACAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 501 GCTCAGC GC^T[ACGT]AGC
CfoI 120 GCGC GCG^C
Cfr10I 801 ACCGGT [GA]^CCGG[CT]
EcoRI 1101 GAATTC G^AATTC
EcoT22I 841 ATGCAT ATGCA^T
HhaI 120 GCGC GCG^C
HinP1I 120 GCGC G^CGC
KpnI 3436 GGTACC GGTAC^C
MamI 88 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 308 TCCGGA T^CCGGA
NcoI 2700 CCATGG C^CATGG
NdeI 1915 CATATG CA^TATG
NheI 4637 GCTAGC G^CTAGC
NsiI 841 ATGCAT ATGCA^T
PflMI 3502 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 801 ACCGGT A^CCGGT
PshAI 5054 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3997 ATTAAT AT^TAAT
SbfI 3312 CCTGCAGG CCTGCA^GG
ScaI 4152 AGTACT AGT^ACT
SexAI 4990 ACCAGGT A^CC[AT]GGT
SphI 426 GCATGC GCATG^C
Sse8387I 3312 CCTGCAGG CCTGCA^GG
SspBI 5238 TGTACA T^GTACA
StuI 3291 AGGCCT AGG^CCT
TaqI 5354 TCGA T^CGA
TthHB8I 5354 TCGA T^CGA
Van91I 3502 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4985 TCTAGA T^CTAGA
XcmI 4642 CCATTTATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 2511 GACGTC G[GA]^CG[CT]C
3031 GGCGTC
AflIII 1417 ACATGT A^C[GA][CT]GT
3515 ACATGT
AocI 3406 CCTGAGG CC^T[ACGT]AGG
4782 CCTGAGG
AspHI 499 GAGCTC G[AT]GC[AT]^C
4973 GAGCTC
AvaII 4608 GGACC G^G[AT]CC
4778 GGTCC
BbvCI 902 GCTGAGG CCTCAGC(-5/-2)
3426 GCTGAGG
BciVI 721 GTATCC GTATCC(6/5)
5256 GTATCC
BglII 125 AGATCT A^GATCT
2901 AGATCT
BpmI 561 CTCCAG CTGGAG(16/14)
4867 CTCCAG
BsaHI 2511 GACGTC G[GA]^CG[CT]C
3031 GGCGTC
BsiHKAI 499 GAGCTC G[AT]GC[AT]^C
4973 GAGCTC
BspHI 2488 TCATGA T^CATGA
4373 TCATGA
BspLU11I 1417 ACATGT A^CATGT
3515 ACATGT
BspMI 876 ACCTGC ACCTGC(4/8)
1175 GCAGGT
BsrDI 245 CATTGC GCAATG(2/0)
4562 GCAATG
Bsu36I 3406 CCTGAGG CC^T[ACGT]AGG
4782 CCTGAGG
DsaI 2700 CCATGG C^C[GA][CT]GG
3281 CCACGG
Eco81I 3406 CCTGAGG CC^T[ACGT]AGG
4782 CCTGAGG
FauI 5013 CCCGC CCCGC(4/6)
3028 GCGGG
HgaI 2735 GACGC GACGC(5/10)
3032 GCGTC
Hin1I 2511 GACGTC G[GA]^CG[CT]C
3031 GGCGTC
HpaI 1983 GTTAAC GTT^AAC
4852 GTTAAC
MfeI 1069 CAATTG C^AATTG
5227 CAATTG
MspA1I 2811 CAGCTG C[AC]G^C[GT]G
3165 CAGCTG
MunI 1069 CAATTG C^AATTG
5227 CAATTG
PstI 1336 CTGCAG CTGCA^G
3313 CTGCAG
PvuII 2811 CAGCTG CAG^CTG
3165 CAGCTG
RcaI 2488 TCATGA T^CATGA
4373 TCATGA
SacI 499 GAGCTC GAGCT^C
4973 GAGCTC
SmlI 1254 CTCAAG C^T[CT][GA]AG
4545 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I BamHI
BanII BbsI BbvI BclI BfaI
BmyI BpuAI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI Bst1107I
BstAPI BstF5I BstNI BstYI BstZ17I
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI