##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AcsI AflII AgeI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI AviII AvrII BbrPI BclI
BfrI BlnI BsiWI BsmI Bsp1407I
BspDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MfeI MluI MunI
NaeI NgoMIV NotI NsiI NspV
PacI PinAI PmaCI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SapI SbfI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1358 TCCGGA T^CCGGA
AclI 134 AACGTT AA^CGTT
ApaI 1331 GGGCCC GGGCC^C
Asp700I 1165 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2938 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 57 TGGCCA TGG^CCA
BcgI 1476 CGAGCTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1242 GTATCC GTATCC(6/5)
BglII 2123 AGATCT A^GATCT
BsaBI 3147 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1358 TCCGGA T^CCGGA
BsiEI 3170 CGACCG CG[GA][CT]^CG
BsmBI 3969 GAGACG CGTCTC(1/5)
BspEI 1358 TCCGGA T^CCGGA
BspLU11I 110 ACATGT A^CATGT
BstXI 3834 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4418 TTTAAA TTT^AAA
EaeI 57 TGGCCA [CT]^GGCC[GA]
MamI 3147 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 57 TGGCCA TGG^CCA
MroI 1358 TCCGGA T^CCGGA
MscI 57 TGGCCA TGG^CCA
NheI 4659 GCTAGC G^CTAGC
NruI 2864 TCGCGA TCG^CGA
PflFI 2938 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 4417 GTTTAAAC GTTT^AAAC
Psp1406I 134 AACGTT AA^CGTT
SfiI 836 GGCCTGAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3575 CCCGGG CCC^GGG
SphI 3194 GCATGC GCATG^C
Tth111I 2938 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4524 TCTAGA T^CTAGA
XmaCI 3575 CCCGGG C^CCGGG
XmaI 3575 CCCGGG C^CCGGG
XmnI 1165 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2398 GGTACC G^GTACC
2421 GGTACC
AccI 1404 GTCTAC GT^[AC][GT]AC
2785 GTAGAC
AccII 253 CGCG CG^CG
2865 CGCG
AhdI 1457 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3974 GACAGCAGGTC
AocI 778 CCTGAGG CC^T[ACGT]AGG
4147 CCTTAGG
Asp718I 2398 GGTACC G^GTACC
2421 GGTACC
AspEI 1457 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3974 GACAGCAGGTC
BbeI 3289 GGCGCC GGCGC^C
4213 GGCGCC
BlpI 429 GCTGAGC GC^T[ACGT]AGC
970 GCTCAGC
Bpu1102I 429 GCTGAGC GC^T[ACGT]AGC
970 GCTCAGC
BsaAI 1073 CACGTA [CT]AC^GT[GA]
1663 TACGTG
BsaWI 1358 TCCGGA [AT]^CCGG[AT]
2651 ACCGGA
BspHI 1032 TCATGA T^CATGA
1389 TCATGA
BstAPI 1824 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3385 GCATCCATTGC
BstUI 253 CGCG CG^CG
2865 CGCG
Bsu36I 778 CCTGAGG CC^T[ACGT]AGG
4147 CCTTAGG
CelII 429 GCTGAGC GC^T[ACGT]AGC
970 GCTCAGC
DraIII 1531 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1628 CACGCAGTG
Eam1105I 1457 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3974 GACAGCAGGTC
EarI 4027 GAAGAG CTCTTC(1/4)
4055 GAAGAG
Eco81I 778 CCTGAGG CC^T[ACGT]AGG
4147 CCTTAGG
EcoRV 2083 GATATC GAT^ATC
2410 GATATC
HincII 2517 GTTGAC GT[CT]^[GA]AC
3259 GTCAAC
HindII 2517 GTTGAC GT[CT]^[GA]AC
3259 GTCAAC
KasI 3289 GGCGCC G^GCGCC
4213 GGCGCC
KpnI 2398 GGTACC GGTAC^C
2421 GGTACC
Ksp632I 4027 GAAGAG CTCTTC(1/4)
4055 GAAGAG
MvnI 253 CGCG CG^CG
2865 CGCG
NarI 3289 GGCGCC GG^CGCC
4213 GGCGCC
NcoI 626 CCATGG C^CATGG
3444 CCATGG
PaeR7I 697 CTCGAG C^TCGAG
791 CTCGAG
PstI 1945 CTGCAG CTGCA^G
3127 CTGCAG
RcaI 1032 TCATGA T^CATGA
1389 TCATGA
ScaI 1259 AGTACT AGT^ACT
4431 AGTACT
SexAI 2417 ACCTGGT A^CC[AT]GGT
2826 ACCTGGT
SfoI 3289 GGCGCC GGC^GCC
4213 GGCGCC
TfiI 2032 GAATC G^A[AT]TC
4601 GAATC
XhoI 697 CTCGAG C^TCGAG
791 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII