##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccII AccIII AclI AgeI AscI
Asp700I BbeI BbrPI BcgI BciVI
BseAI BsiEI BsmI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3820 GACGTC GACGT^C
Acc65I 155 GGTACC G^GTACC
AccI 3540 GTAGAC GT^[AC][GT]AC
Alw44I 5041 GTGCAC G^TGCAC
Aor51HI 4998 AGCGCT AGC^GCT
ApaI 122 GGGCCC GGGCC^C
ApaLI 5041 GTGCAC G^TGCAC
AseI 4308 ATTAAT AT^TAAT
AsnI 4308 ATTAAT AT^TAAT
Asp718I 155 GGTACC G^GTACC
AspI 4380 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3890 CCTAGG C^CTAGG
BalI 1688 TGGCCA TGG^CCA
BamHI 1512 GGATCC G^GATCC
BglI 1520 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3890 CCTAGG C^CTAGG
BsaBI 3238 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 1187 CGTACG C^GTACG
BspHI 1018 TCATGA T^CATGA
BspLU11I 3941 ACATGT A^CATGT
BsrFI 609 GCCGGC [GA]^CCGG[CT]
Cfr10I 609 GCCGGC [GA]^CCGG[CT]
Eco47III 4998 AGCGCT AGC^GCT
EcoRI 1139 GAATTC G^AATTC
HincII 3276 GTTGAC GT[CT]^[GA]AC
HindII 3276 GTTGAC GT[CT]^[GA]AC
KpnI 155 GGTACC GGTAC^C
MamI 3238 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1688 TGGCCA TGG^CCA
MscI 1688 TGGCCA TGG^CCA
NaeI 609 GCCGGC GCC^GGC
NgoMIV 609 GCCGGC G^CCGGC
PaeR7I 1642 CTCGAG C^TCGAG
PflFI 4380 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4308 ATTAAT AT^TAAT
RcaI 1018 TCATGA T^CATGA
SphI 2382 GCATGC GCATG^C
Tth111I 4380 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1642 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 599 GGCGTC G[GA]^CG[CT]C
3820 GACGTC
AflII 3834 CTTAAG C^TTAAG
4287 CTTAAG
AhdI 3820 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4489 GACAGAAAGTC
AspEI 3820 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4489 GACAGAAAGTC
AvaI 1642 CTCGAG C^[CT]CG[GA]G
4716 CTCGGG
AviII 1246 TGCGCA TGC^GCA
4993 TGCGCA
BbvCI 542 CCTCAGC CCTCAGC(-5/-2)
3197 GCTGAGG
BclI 1260 TGATCA T^GATCA
3492 TGATCA
BcnI 199 CCGGG CC^[GC]GG
589 CCCGG
BfrI 3834 CTTAAG C^TTAAG
4287 CTTAAG
BsaHI 599 GGCGTC G[GA]^CG[CT]C
3820 GACGTC
BsaI 4378 GAGACC GGTCTC(1/5)
3378 GGTCTC
BsaWI 261 TCCGGT [AT]^CCGG[AT]
778 ACCGGA
BsmBI 700 CGTCTC CGTCTC(1/5)
3954 GAGACG
BsoBI 1642 CTCGAG C^[CT]CG[GA]G
4716 CTCGGG
BsrBI 1858 CCGCTC CCGCTC(-3/-3)
1508 GAGCGG
BssSI 405 CTCGTG CACGAG(-5/-1)
3113 CTCGTG
DsaI 2173 CCGTGG C^C[GA][CT]GG
2238 CCGTGG
Eam1105I 3820 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4489 GACAGAAAGTC
FauI 171 CCCGC CCCGC(4/6)
1857 CCCGC
FbaI 1260 TGATCA T^GATCA
3492 TGATCA
FspI 1246 TGCGCA TGC^GCA
4993 TGCGCA
HaeII 2371 GGCGCT [GA]GCGC^[CT]
4998 AGCGCT
Hin1I 599 GGCGTC G[GA]^CG[CT]C
3820 GACGTC
HindIII 2897 AAGCTT A^AGCTT
4929 AAGCTT
NciI 199 CCGGG CC^[GC]GG
589 CCCGG
PflMI 514 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
808 CCAGCAAATGG
ScaI 4148 AGTACT AGT^ACT
4779 AGTACT
StuI 927 AGGCCT AGG^CCT
1850 AGGCCT
Van91I 514 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
808 CCAGCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII