################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AccII AccIII AclI AgeI AscI Asp700I BbeI BbrPI BcgI BciVI BseAI BsiEI BsmI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI HpaI KasI KspI MfeI MluI MroI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 3820 GACGTC GACGT^C Acc65I 155 GGTACC G^GTACC AccI 3540 GTAGAC GT^[AC][GT]AC Alw44I 5041 GTGCAC G^TGCAC Aor51HI 4998 AGCGCT AGC^GCT ApaI 122 GGGCCC GGGCC^C ApaLI 5041 GTGCAC G^TGCAC AseI 4308 ATTAAT AT^TAAT AsnI 4308 ATTAAT AT^TAAT Asp718I 155 GGTACC G^GTACC AspI 4380 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3890 CCTAGG C^CTAGG BalI 1688 TGGCCA TGG^CCA BamHI 1512 GGATCC G^GATCC BglI 1520 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3890 CCTAGG C^CTAGG BsaBI 3238 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 1187 CGTACG C^GTACG BspHI 1018 TCATGA T^CATGA BspLU11I 3941 ACATGT A^CATGT BsrFI 609 GCCGGC [GA]^CCGG[CT] Cfr10I 609 GCCGGC [GA]^CCGG[CT] Eco47III 4998 AGCGCT AGC^GCT EcoRI 1139 GAATTC G^AATTC HincII 3276 GTTGAC GT[CT]^[GA]AC HindII 3276 GTTGAC GT[CT]^[GA]AC KpnI 155 GGTACC GGTAC^C MamI 3238 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1688 TGGCCA TGG^CCA MscI 1688 TGGCCA TGG^CCA NaeI 609 GCCGGC GCC^GGC NgoMIV 609 GCCGGC G^CCGGC PaeR7I 1642 CTCGAG C^TCGAG PflFI 4380 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 4308 ATTAAT AT^TAAT RcaI 1018 TCATGA T^CATGA SphI 2382 GCATGC GCATG^C Tth111I 4380 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1642 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 599 GGCGTC G[GA]^CG[CT]C 3820 GACGTC AflII 3834 CTTAAG C^TTAAG 4287 CTTAAG AhdI 3820 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4489 GACAGAAAGTC AspEI 3820 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4489 GACAGAAAGTC AvaI 1642 CTCGAG C^[CT]CG[GA]G 4716 CTCGGG AviII 1246 TGCGCA TGC^GCA 4993 TGCGCA BbvCI 542 CCTCAGC CCTCAGC(-5/-2) 3197 GCTGAGG BclI 1260 TGATCA T^GATCA 3492 TGATCA BcnI 199 CCGGG CC^[GC]GG 589 CCCGG BfrI 3834 CTTAAG C^TTAAG 4287 CTTAAG BsaHI 599 GGCGTC G[GA]^CG[CT]C 3820 GACGTC BsaI 4378 GAGACC GGTCTC(1/5) 3378 GGTCTC BsaWI 261 TCCGGT [AT]^CCGG[AT] 778 ACCGGA BsmBI 700 CGTCTC CGTCTC(1/5) 3954 GAGACG BsoBI 1642 CTCGAG C^[CT]CG[GA]G 4716 CTCGGG BsrBI 1858 CCGCTC CCGCTC(-3/-3) 1508 GAGCGG BssSI 405 CTCGTG CACGAG(-5/-1) 3113 CTCGTG DsaI 2173 CCGTGG C^C[GA][CT]GG 2238 CCGTGG Eam1105I 3820 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4489 GACAGAAAGTC FauI 171 CCCGC CCCGC(4/6) 1857 CCCGC FbaI 1260 TGATCA T^GATCA 3492 TGATCA FspI 1246 TGCGCA TGC^GCA 4993 TGCGCA HaeII 2371 GGCGCT [GA]GCGC^[CT] 4998 AGCGCT Hin1I 599 GGCGTC G[GA]^CG[CT]C 3820 GACGTC HindIII 2897 AAGCTT A^AGCTT 4929 AAGCTT NciI 199 CCGGG CC^[GC]GG 589 CCCGG PflMI 514 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 808 CCAGCAAATGG ScaI 4148 AGTACT AGT^ACT 4779 AGTACT StuI 927 AGGCCT AGG^CCT 1850 AGGCCT Van91I 514 CCATGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 808 CCAGCAAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbsI BbvI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII