##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII AccIII AgeI AscI AseI
AsnI AvrII BbeI BclI BlnI
BseAI BsiWI BspDI BspEI BspHI
BspMI BstBI BstEII BstPI ClaI
CpoI DraIII DrdI EcoO65I EcoRI
FbaI KasI KspI MfeI MluI
MroI MunI NarI NotI NspV
PacI PinAI PmeI PshBI PvuI
RcaI RsrII SacII SalI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 249 GGTACC G^GTACC
AclI 716 AACGTT AA^CGTT
AcyI 4431 GACGCC G[GA]^CG[CT]C
AflII 1170 CTTAAG C^TTAAG
Aor51HI 529 AGCGCT AGC^GCT
Asp700I 1187 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 249 GGTACC G^GTACC
AspI 2469 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3399 CGAAACGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2977 GTATCC GTATCC(6/5)
BfrI 1170 CTTAAG C^TTAAG
BglI 3567 GCCAAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1351 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4431 GACGCC G[GA]^CG[CT]C
BsaWI 369 TCCGGT [AT]^CCGG[AT]
BsgI 3453 CTGCAC GTGCAG(16/14)
BspLU11I 2193 ACATGT A^CATGT
BsrBI 3087 GAGCGG CCGCTC(-3/-3)
BsrDI 994 CATTGC GCAATG(2/0)
BssHII 2850 GCGCGC G^CGCGC
EagI 3680 CGGCCG C^GGCCG
EclXI 3680 CGGCCG C^GGCCG
Eco47III 529 AGCGCT AGC^GCT
Eco52I 3680 CGGCCG C^GGCCG
EcoRV 141 GATATC GAT^ATC
FseI 3677 GGCCGGCC GGCCGG^CC
Hin1I 4431 GACGCC G[GA]^CG[CT]C
KpnI 249 GGTACC GGTAC^C
MamI 1351 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1211 CATATG CA^TATG
NruI 3368 TCGCGA TCG^CGA
PflFI 2469 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 716 AACGTT AA^CGTT
SbfI 1557 CCTGCAGG CCTGCA^GG
ScaI 183 AGTACT AGT^ACT
SexAI 3469 ACCTGGT A^CC[AT]GGT
SmaI 3295 CCCGGG CCC^GGG
Sse8387I 1557 CCTGCAGG CCTGCA^GG
SwaI 915 ATTTAAAT ATTT^AAAT
Tth111I 2469 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1606 TCTAGA T^CTAGA
XmaCI 3295 CCCGGG C^CCGGG
XmaI 3295 CCCGGG C^CCGGG
XmnI 1187 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 3468 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3856 GACAGGAAGTC
ApaI 3668 GGGCCC GGGCC^C
4040 GGGCCC
AspEI 3468 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3856 GACAGGAAGTC
BalI 939 TGGCCA TGG^CCA
3751 TGGCCA
BglII 10 AGATCT A^GATCT
1080 AGATCT
BsmBI 3745 CGTCTC CGTCTC(1/5)
4429 GAGACG
Bsp1407I 79 TGTACA T^GTACA
1215 TGTACA
BsrGI 79 TGTACA T^GTACA
1215 TGTACA
Bst1107I 2685 GTATAC GTA^TAC
2695 GTATAC
BstZ17I 2685 GTATAC GTA^TAC
2695 GTATAC
Eam1105I 3468 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3856 GACAGGAAGTC
EcoT22I 1313 ATGCAT ATGCA^T
3264 ATGCAT
HindIII 1539 AAGCTT A^AGCTT
4605 AAGCTT
MluNI 939 TGGCCA TGG^CCA
3751 TGGCCA
MscI 939 TGGCCA TGG^CCA
3751 TGGCCA
NheI 862 GCTAGC G^CTAGC
2653 GCTAGC
NsiI 1313 ATGCAT ATGCA^T
3264 ATGCAT
PaeR7I 101 CTCGAG C^TCGAG
2668 CTCGAG
PshAI 455 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4493 GACCCCAGTC
SphI 1315 GCATGC GCATG^C
3262 GCATGC
SspBI 79 TGTACA T^GTACA
1215 TGTACA
StuI 572 AGGCCT AGG^CCT
1633 AGGCCT
XhoI 101 CTCGAG C^TCGAG
2668 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AvaI
AvaII AviII BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII