##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I CpoI DraI DraIII
Eco47III Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 977 AAATTC [GA]^AATT[CT]
AcyI 173 GGCGCC G[GA]^CG[CT]C
AlwI 1832 GGATC GGATC(4/5)
AlwNI 2036 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 977 AAATTC [GA]^AATT[CT]
AvaI 99 CCCGAG C^[CT]CG[GA]G
BbeI 173 GGCGCC GGCGC^C
BbsI 1057 GTCTTC GAAGAC(2/6)
BbvCI 135 CCTCAGC CCTCAGC(-5/-2)
BcnI 153 CCCGG CC^[GC]GG
BglII 1301 AGATCT A^GATCT
BlpI 897 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 897 GCTTAGC GC^T[ACGT]AGC
BpuAI 1057 GTCTTC GAAGAC(2/6)
BsaBI 1220 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 173 GGCGCC G[GA]^CG[CT]C
BsgI 385 CTGCAC GTGCAG(16/14)
BsiEI 1557 CGGCCG CG[GA][CT]^CG
BsoBI 99 CCCGAG C^[CT]CG[GA]G
BspDI 996 ATCGAT AT^CGAT
BsrDI 360 CATTGC GCAATG(2/0)
BstXI 471 CCATTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 897 GCTTAGC GC^T[ACGT]AGC
ClaI 996 ATCGAT AT^CGAT
DrdI 1774 GACCTCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1557 CGGCCG C^GGCCG
EclXI 1557 CGGCCG C^GGCCG
Eco52I 1557 CGGCCG C^GGCCG
EcoNI 790 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1129 ATGCAT ATGCA^T
HaeII 173 GGCGCC [GA]GCGC^[CT]
Hin1I 173 GGCGCC G[GA]^CG[CT]C
KasI 173 GGCGCC G^GCGCC
MamI 1220 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 692 CAGCGG C[AC]G^C[GT]G
NarI 173 GGCGCC GG^CGCC
NciI 153 CCCGG CC^[GC]GG
NsiI 1129 ATGCAT ATGCA^T
PstI 222 CTGCAG CTGCA^G
SexAI 2250 ACCAGGT A^CC[AT]GGT
SfoI 173 GGCGCC GGC^GCC
XbaI 836 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 12 GTAGAC GT^[AC][GT]AC
2246 GTAGAC
AfaI 414 GTAC GT^AC
1233 GTAC
AhdI 1483 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1960 GACAGAAGGTC
Alw44I 534 GTGCAC G^TGCAC
2006 GTGCAC
ApaLI 534 GTGCAC G^TGCAC
2006 GTGCAC
AspEI 1483 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1960 GACAGAAGGTC
BglI 64 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1179 GCCCCGATGGC
BsmI 53 GAATGC GAATGC(1/-1)
1131 GCATTC
BstAPI 432 GCAAACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2022 GCATGTGGTGC
BstYI 1301 AGATCT [GA]^GATC[CT]
1832 GGATCT
CfoI 174 GCGC GCG^C
2320 GCGC
Eam1105I 1483 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1960 GACAGAAGGTC
EarI 870 CTCTTC CTCTTC(1/4)
644 GAAGAG
FauI 722 GCGGG CCCGC(4/6)
802 GCGGG
HapII 154 CCGG C^CGG
1556 CCGG
HgaI 649 GCGTC GACGC(5/10)
833 GCGTC
HhaI 174 GCGC GCG^C
2320 GCGC
HinP1I 174 GCGC G^CGC
2320 GCGC
HpaII 154 CCGG C^CGG
1556 CCGG
HphI 1548 GGTGA GGTGA(8/7)
233 TCACC
Ksp632I 870 CTCTTC CTCTTC(1/4)
644 GAAGAG
MaeII 281 ACGT A^CGT
1781 ACGT
MflI 1301 AGATCT [GA]^GATC[CT]
1832 GGATCT
MspI 154 CCGG C^CGG
1556 CCGG
NcoI 1073 CCATGG C^CATGG
1871 CCATGG
PflMI 74 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2297 CCAAGTGCTGG
RsaI 414 GTAC GT^AC
1233 GTAC
SacI 767 GAGCTC GAGCT^C
867 GAGCTC
SapI 644 GAAGAGC GCTCTTC(1/4)
869 GCTCTTC
SfcI 10 CTGTAG C^T[GA][CT]AG
222 CTGCAG
SphI 1127 GCATGC GCATG^C
1495 GCATGC
TfiI 840 GATTC G^A[AT]TC
2113 GAATC
Tsp45I 1600 GTCAC ^GT[GC]AC
2152 GTCAC
Van91I 74 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2297 CCAAGTGCTGG
XcmI 1274 CCATGCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1918 CCAAGAAGCTCCTGG
XhoII 1301 AGATCT [GA]^GATC[CT]
1832 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI ApaI AspHI AvaII
BalI BanI BanII BbvI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I