################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BlnI BsaAI BsaWI BseAI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII Eco47III Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 977 AAATTC [GA]^AATT[CT] AcyI 173 GGCGCC G[GA]^CG[CT]C AlwI 1832 GGATC GGATC(4/5) AlwNI 2036 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 977 AAATTC [GA]^AATT[CT] AvaI 99 CCCGAG C^[CT]CG[GA]G BbeI 173 GGCGCC GGCGC^C BbsI 1057 GTCTTC GAAGAC(2/6) BbvCI 135 CCTCAGC CCTCAGC(-5/-2) BcnI 153 CCCGG CC^[GC]GG BglII 1301 AGATCT A^GATCT BlpI 897 GCTTAGC GC^T[ACGT]AGC Bpu1102I 897 GCTTAGC GC^T[ACGT]AGC BpuAI 1057 GTCTTC GAAGAC(2/6) BsaBI 1220 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 173 GGCGCC G[GA]^CG[CT]C BsgI 385 CTGCAC GTGCAG(16/14) BsiEI 1557 CGGCCG CG[GA][CT]^CG BsoBI 99 CCCGAG C^[CT]CG[GA]G BspDI 996 ATCGAT AT^CGAT BsrDI 360 CATTGC GCAATG(2/0) BstXI 471 CCATTACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 897 GCTTAGC GC^T[ACGT]AGC ClaI 996 ATCGAT AT^CGAT DrdI 1774 GACCTCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1557 CGGCCG C^GGCCG EclXI 1557 CGGCCG C^GGCCG Eco52I 1557 CGGCCG C^GGCCG EcoNI 790 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1129 ATGCAT ATGCA^T HaeII 173 GGCGCC [GA]GCGC^[CT] Hin1I 173 GGCGCC G[GA]^CG[CT]C KasI 173 GGCGCC G^GCGCC MamI 1220 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 692 CAGCGG C[AC]G^C[GT]G NarI 173 GGCGCC GG^CGCC NciI 153 CCCGG CC^[GC]GG NsiI 1129 ATGCAT ATGCA^T PstI 222 CTGCAG CTGCA^G SexAI 2250 ACCAGGT A^CC[AT]GGT SfoI 173 GGCGCC GGC^GCC XbaI 836 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 12 GTAGAC GT^[AC][GT]AC 2246 GTAGAC AfaI 414 GTAC GT^AC 1233 GTAC AhdI 1483 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1960 GACAGAAGGTC Alw44I 534 GTGCAC G^TGCAC 2006 GTGCAC ApaLI 534 GTGCAC G^TGCAC 2006 GTGCAC AspEI 1483 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1960 GACAGAAGGTC BglI 64 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1179 GCCCCGATGGC BsmI 53 GAATGC GAATGC(1/-1) 1131 GCATTC BstAPI 432 GCAAACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2022 GCATGTGGTGC BstYI 1301 AGATCT [GA]^GATC[CT] 1832 GGATCT CfoI 174 GCGC GCG^C 2320 GCGC Eam1105I 1483 GACTTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1960 GACAGAAGGTC EarI 870 CTCTTC CTCTTC(1/4) 644 GAAGAG FauI 722 GCGGG CCCGC(4/6) 802 GCGGG HapII 154 CCGG C^CGG 1556 CCGG HgaI 649 GCGTC GACGC(5/10) 833 GCGTC HhaI 174 GCGC GCG^C 2320 GCGC HinP1I 174 GCGC G^CGC 2320 GCGC HpaII 154 CCGG C^CGG 1556 CCGG HphI 1548 GGTGA GGTGA(8/7) 233 TCACC Ksp632I 870 CTCTTC CTCTTC(1/4) 644 GAAGAG MaeII 281 ACGT A^CGT 1781 ACGT MflI 1301 AGATCT [GA]^GATC[CT] 1832 GGATCT MspI 154 CCGG C^CGG 1556 CCGG NcoI 1073 CCATGG C^CATGG 1871 CCATGG PflMI 74 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2297 CCAAGTGCTGG RsaI 414 GTAC GT^AC 1233 GTAC SacI 767 GAGCTC GAGCT^C 867 GAGCTC SapI 644 GAAGAGC GCTCTTC(1/4) 869 GCTCTTC SfcI 10 CTGTAG C^T[GA][CT]AG 222 CTGCAG SphI 1127 GCATGC GCATG^C 1495 GCATGC TfiI 840 GATTC G^A[AT]TC 2113 GAATC Tsp45I 1600 GTCAC ^GT[GC]AC 2152 GTCAC Van91I 74 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2297 CCAAGTGCTGG XcmI 1274 CCATGCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1918 CCAAGAAGCTCCTGG XhoII 1301 AGATCT [GA]^GATC[CT] 1832 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AluI ApaI AspHI AvaII BalI BanI BanII BbvI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I