################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AocI ApaI AscI Asp718I BanI BbeI BbrPI BglI BsaHI BsaWI BseAI BsiWI BspEI BsrBI BssHII BstAPI Bsu36I CpoI DrdI EagI EclXI Eco52I Eco81I EcoRV FseI Hin1I HincII HindII HpaI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 3309 CGCG CG^CG Alw44I 5484 GTGCAC G^TGCAC Aor51HI 806 AGCGCT AGC^GCT ApaLI 5484 GTGCAC G^TGCAC AspI 3351 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 695 CTCGGG C^[CT]CG[GA]G AviII 3294 TGCGCA TGC^GCA BcgI 57 CGAGAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2169 GGATAC GTATCC(6/5) BclI 1547 TGATCA T^GATCA BlpI 4167 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4167 GCTCAGC GC^T[ACGT]AGC BsaBI 370 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 10 CGGTCG CG[GA][CT]^CG BsmBI 1387 GAGACG CGTCTC(1/5) BsoBI 695 CTCGGG C^[CT]CG[GA]G BspDI 367 ATCGAT AT^CGAT BsrFI 36 GCCGGT [GA]^CCGG[CT] BssSI 4127 CTCGTG CACGAG(-5/-1) BstBI 1983 TTCGAA TT^CGAA BstUI 3309 CGCG CG^CG CelII 4167 GCTCAGC GC^T[ACGT]AGC Cfr10I 36 GCCGGT [GA]^CCGG[CT] ClaI 367 ATCGAT AT^CGAT DraIII 5681 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 806 AGCGCT AGC^GCT EcoNI 4831 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2944 GAATTC G^AATTC FbaI 1547 TGATCA T^GATCA FspI 3294 TGCGCA TGC^GCA HaeII 806 AGCGCT [GA]GCGC^[CT] MamI 370 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2498 CAATTG C^AATTG MunI 2498 CAATTG C^AATTG MvnI 3309 CGCG CG^CG NheI 3162 GCTAGC G^CTAGC NspV 1983 TTCGAA TT^CGAA PflFI 3351 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ScaI 6137 AGTACT AGT^ACT SfuI 1983 TTCGAA TT^CGAA SnaBI 4550 TACGTA TAC^GTA SwaI 264 ATTTAAAT ATTT^AAAT Tth111I 3351 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AflII 830 CTTAAG C^TTAAG 4559 CTTAAG AhdI 568 GACTGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1558 GACAGTCTGTC AlwNI 1068 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1266 CAGAAGCTG AspEI 568 GACTGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1558 GACAGTCTGTC BamHI 3127 GGATCC G^GATCC 5745 GGATCC BbvCI 1037 CCTCAGC CCTCAGC(-5/-2) 4136 GCTGAGG BfrI 830 CTTAAG C^TTAAG 4559 CTTAAG BsaAI 2456 TACGTG [CT]AC^GT[GA] 4550 TACGTA BsaI 1497 GAGACC GGTCTC(1/5) 5537 GAGACC BseRI 442 GAGGAG GAGGAG(10/8) 1255 GAGGAG BsgI 5282 CTGCAC GTGCAG(16/14) 5837 CTGCAC BsmI 1810 GCATTC GAATGC(1/-1) 2346 GCATTC Bsp1407I 1907 TGTACA T^GTACA 3314 TGTACA BspMI 2591 ACCTGC ACCTGC(4/8) 2278 GCAGGT BsrDI 3166 GCAATG GCAATG(2/0) 3958 GCAATG BsrGI 1907 TGTACA T^GTACA 3314 TGTACA BstEII 1656 GGTCACC G^GT[ACGT]ACC 3984 GGTTACC BstPI 1656 GGTCACC G^GT[ACGT]ACC 3984 GGTTACC BstXI 243 CCACAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4113 CCAGTCTGTTGG Eam1105I 568 GACTGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1558 GACAGTCTGTC EcoO65I 1656 GGTCACC G^GT[ACGT]ACC 3984 GGTTACC FauI 1240 CCCGC CCCGC(4/6) 1279 GCGGG HindIII 2519 AAGCTT A^AGCTT 4755 AAGCTT MspA1I 1071 CAGCTG C[AC]G^C[GT]G 1584 CAGCTG NcoI 3227 CCATGG C^CATGG 3323 CCATGG PvuII 1071 CAGCTG CAG^CTG 1584 CAGCTG SacI 4165 GAGCTC GAGCT^C 4266 GAGCTC SapI 4 GCTCTTC GCTCTTC(1/4) 1597 GCTCTTC SspBI 1907 TGTACA T^GTACA 3314 TGTACA SspI 1875 AATATT AAT^ATT 3194 AATATT StuI 3302 AGGCCT AGG^CCT 4324 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII AvrII BalI BanII BbsI BbvI BcnI BfaI BglII BlnI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspLU11I BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII XmnI