##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AocI ApaI AscI Asp718I
BanI BbeI BbrPI BglI BsaHI
BsaWI BseAI BsiWI BspEI BsrBI
BssHII BstAPI Bsu36I CpoI DrdI
EagI EclXI Eco52I Eco81I EcoRV
FseI Hin1I HincII HindII HpaI
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 3309 CGCG CG^CG
Alw44I 5484 GTGCAC G^TGCAC
Aor51HI 806 AGCGCT AGC^GCT
ApaLI 5484 GTGCAC G^TGCAC
AspI 3351 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 695 CTCGGG C^[CT]CG[GA]G
AviII 3294 TGCGCA TGC^GCA
BcgI 57 CGAGAGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2169 GGATAC GTATCC(6/5)
BclI 1547 TGATCA T^GATCA
BlpI 4167 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4167 GCTCAGC GC^T[ACGT]AGC
BsaBI 370 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 10 CGGTCG CG[GA][CT]^CG
BsmBI 1387 GAGACG CGTCTC(1/5)
BsoBI 695 CTCGGG C^[CT]CG[GA]G
BspDI 367 ATCGAT AT^CGAT
BsrFI 36 GCCGGT [GA]^CCGG[CT]
BssSI 4127 CTCGTG CACGAG(-5/-1)
BstBI 1983 TTCGAA TT^CGAA
BstUI 3309 CGCG CG^CG
CelII 4167 GCTCAGC GC^T[ACGT]AGC
Cfr10I 36 GCCGGT [GA]^CCGG[CT]
ClaI 367 ATCGAT AT^CGAT
DraIII 5681 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 806 AGCGCT AGC^GCT
EcoNI 4831 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2944 GAATTC G^AATTC
FbaI 1547 TGATCA T^GATCA
FspI 3294 TGCGCA TGC^GCA
HaeII 806 AGCGCT [GA]GCGC^[CT]
MamI 370 GATATAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2498 CAATTG C^AATTG
MunI 2498 CAATTG C^AATTG
MvnI 3309 CGCG CG^CG
NheI 3162 GCTAGC G^CTAGC
NspV 1983 TTCGAA TT^CGAA
PflFI 3351 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 6137 AGTACT AGT^ACT
SfuI 1983 TTCGAA TT^CGAA
SnaBI 4550 TACGTA TAC^GTA
SwaI 264 ATTTAAAT ATTT^AAAT
Tth111I 3351 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflII 830 CTTAAG C^TTAAG
4559 CTTAAG
AhdI 568 GACTGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACAGTCTGTC
AlwNI 1068 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1266 CAGAAGCTG
AspEI 568 GACTGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACAGTCTGTC
BamHI 3127 GGATCC G^GATCC
5745 GGATCC
BbvCI 1037 CCTCAGC CCTCAGC(-5/-2)
4136 GCTGAGG
BfrI 830 CTTAAG C^TTAAG
4559 CTTAAG
BsaAI 2456 TACGTG [CT]AC^GT[GA]
4550 TACGTA
BsaI 1497 GAGACC GGTCTC(1/5)
5537 GAGACC
BseRI 442 GAGGAG GAGGAG(10/8)
1255 GAGGAG
BsgI 5282 CTGCAC GTGCAG(16/14)
5837 CTGCAC
BsmI 1810 GCATTC GAATGC(1/-1)
2346 GCATTC
Bsp1407I 1907 TGTACA T^GTACA
3314 TGTACA
BspMI 2591 ACCTGC ACCTGC(4/8)
2278 GCAGGT
BsrDI 3166 GCAATG GCAATG(2/0)
3958 GCAATG
BsrGI 1907 TGTACA T^GTACA
3314 TGTACA
BstEII 1656 GGTCACC G^GT[ACGT]ACC
3984 GGTTACC
BstPI 1656 GGTCACC G^GT[ACGT]ACC
3984 GGTTACC
BstXI 243 CCACAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4113 CCAGTCTGTTGG
Eam1105I 568 GACTGTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACAGTCTGTC
EcoO65I 1656 GGTCACC G^GT[ACGT]ACC
3984 GGTTACC
FauI 1240 CCCGC CCCGC(4/6)
1279 GCGGG
HindIII 2519 AAGCTT A^AGCTT
4755 AAGCTT
MspA1I 1071 CAGCTG C[AC]G^C[GT]G
1584 CAGCTG
NcoI 3227 CCATGG C^CATGG
3323 CCATGG
PvuII 1071 CAGCTG CAG^CTG
1584 CAGCTG
SacI 4165 GAGCTC GAGCT^C
4266 GAGCTC
SapI 4 GCTCTTC GCTCTTC(1/4)
1597 GCTCTTC
SspBI 1907 TGTACA T^GTACA
3314 TGTACA
SspI 1875 AATATT AAT^ATT
3194 AATATT
StuI 3302 AGGCCT AGG^CCT
4324 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AvaII AvrII BalI
BanII BbsI BbvI BcnI BfaI
BglII BlnI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspLU11I BsrI
BssKI Bst1107I BstF5I BstNI BstYI
BstZ17I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII XmnI