################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII BbeI BbrPI BcgI BciVI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 213 AAATTT [GA]^AATT[CT] AocI 370 CCTCAGG CC^T[ACGT]AGG Aor51HI 678 AGCGCT AGC^GCT ApaI 1604 GGGCCC GGGCC^C ApoI 213 AAATTT [GA]^AATT[CT] Asp700I 1593 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 389 GGATCC G^GATCC BclI 1845 TGATCA T^GATCA BsiEI 1010 CGATCG CG[GA][CT]^CG BsmBI 1314 CGTCTC CGTCTC(1/5) BsmI 357 GAATGC GAATGC(1/-1) Bsp1407I 581 TGTACA T^GTACA BsrBI 1785 CCGCTC CCGCTC(-3/-3) BsrGI 581 TGTACA T^GTACA Bsu36I 370 CCTCAGG CC^T[ACGT]AGG DrdI 1774 GACCCTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 678 AGCGCT AGC^GCT Eco81I 370 CCTCAGG CC^T[ACGT]AGG FbaI 1845 TGATCA T^GATCA HaeII 678 AGCGCT [GA]GCGC^[CT] HincII 1861 GTCAAC GT[CT]^[GA]AC HindII 1861 GTCAAC GT[CT]^[GA]AC HindIII 252 AAGCTT A^AGCTT NcoI 2078 CCATGG C^CATGG NdeI 2161 CATATG CA^TATG NspI 702 ACATGC [GA]CATG^[CT] PaeR7I 1790 CTCGAG C^TCGAG PpuMI 1382 GGGACCC [GA]G^G[AT]CC[CT] PvuI 1010 CGATCG CGAT^CG SacI 418 GAGCTC GAGCT^C SexAI 1734 ACCAGGT A^CC[AT]GGT SmaI 1303 CCCGGG CCC^GGG SspBI 581 TGTACA T^GTACA StuI 1104 AGGCCT AGG^CCT XcmI 443 CCAACAAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1790 CTCGAG C^TCGAG XmaCI 1303 CCCGGG C^CCGGG XmaI 1303 CCCGGG C^CCGGG XmnI 1593 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AcyI 742 GACGCC G[GA]^CG[CT]C 1432 GACGCC AspI 14 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1656 GACCTTGTC AvrII 99 CCTAGG C^CTAGG 1909 CCTAGG BalI 1021 TGGCCA TGG^CCA 1230 TGGCCA BbsI 636 GAAGAC GAAGAC(2/6) 892 GTCTTC BbvCI 29 CCTCAGC CCTCAGC(-5/-2) 1437 CCTCAGC BglII 845 AGATCT A^GATCT 2426 AGATCT BlnI 99 CCTAGG C^CTAGG 1909 CCTAGG BpmI 1628 CTCCAG CTGGAG(16/14) 1764 CTGGAG BpuAI 636 GAAGAC GAAGAC(2/6) 892 GTCTTC BsaHI 742 GACGCC G[GA]^CG[CT]C 1432 GACGCC BsaWI 221 ACCGGA [AT]^CCGG[AT] 632 ACCGGA BsgI 2068 CTGCAC GTGCAG(16/14) 2224 GTGCAG BspHI 1523 TCATGA T^CATGA 2474 TCATGA BspMI 2148 ACCTGC ACCTGC(4/8) 115 GCAGGT BsrDI 1032 CATTGC GCAATG(2/0) 323 GCAATG BstXI 1960 CCATCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2078 CCATGGAGATGG CfoI 679 GCGC GCG^C 2468 GCGC DsaI 1200 CCGTGG C^C[GA][CT]GG 2078 CCATGG EcoNI 423 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2115 CCTGGCTCAGG FauI 34 GCGGG CCCGC(4/6) 992 GCGGG HgaI 742 GACGC GACGC(5/10) 1432 GACGC HhaI 679 GCGC GCG^C 2468 GCGC Hin1I 742 GACGCC G[GA]^CG[CT]C 1432 GACGCC HinP1I 679 GCGC G^CGC 2468 GCGC MluNI 1021 TGGCCA TGG^CCA 1230 TGGCCA MscI 1021 TGGCCA TGG^CCA 1230 TGGCCA MseI 629 TTAA T^TAA 2458 TTAA PflFI 14 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1656 GACCTTGTC RcaI 1523 TCATGA T^CATGA 2474 TCATGA TfiI 1397 GATTC G^A[AT]TC 1624 GAATC Tru9I 629 TTAA T^TAA 2458 TTAA Tth111I 14 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1656 GACCTTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII