##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AviII BbeI BbrPI
BcgI BciVI BfrI BglI BlpI
Bpu1102I BsaAI BsaBI BseAI BsiWI
BspDI BspEI BspLU11I BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcsI 213 AAATTT [GA]^AATT[CT]
AocI 370 CCTCAGG CC^T[ACGT]AGG
Aor51HI 678 AGCGCT AGC^GCT
ApaI 1604 GGGCCC GGGCC^C
ApoI 213 AAATTT [GA]^AATT[CT]
Asp700I 1593 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 389 GGATCC G^GATCC
BclI 1845 TGATCA T^GATCA
BsiEI 1010 CGATCG CG[GA][CT]^CG
BsmBI 1314 CGTCTC CGTCTC(1/5)
BsmI 357 GAATGC GAATGC(1/-1)
Bsp1407I 581 TGTACA T^GTACA
BsrBI 1785 CCGCTC CCGCTC(-3/-3)
BsrGI 581 TGTACA T^GTACA
Bsu36I 370 CCTCAGG CC^T[ACGT]AGG
DrdI 1774 GACCCTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 678 AGCGCT AGC^GCT
Eco81I 370 CCTCAGG CC^T[ACGT]AGG
FbaI 1845 TGATCA T^GATCA
HaeII 678 AGCGCT [GA]GCGC^[CT]
HincII 1861 GTCAAC GT[CT]^[GA]AC
HindII 1861 GTCAAC GT[CT]^[GA]AC
HindIII 252 AAGCTT A^AGCTT
NcoI 2078 CCATGG C^CATGG
NdeI 2161 CATATG CA^TATG
NspI 702 ACATGC [GA]CATG^[CT]
PaeR7I 1790 CTCGAG C^TCGAG
PpuMI 1382 GGGACCC [GA]G^G[AT]CC[CT]
PvuI 1010 CGATCG CGAT^CG
SacI 418 GAGCTC GAGCT^C
SexAI 1734 ACCAGGT A^CC[AT]GGT
SmaI 1303 CCCGGG CCC^GGG
SspBI 581 TGTACA T^GTACA
StuI 1104 AGGCCT AGG^CCT
XcmI 443 CCAACAAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1790 CTCGAG C^TCGAG
XmaCI 1303 CCCGGG C^CCGGG
XmaI 1303 CCCGGG C^CCGGG
XmnI 1593 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 742 GACGCC G[GA]^CG[CT]C
1432 GACGCC
AspI 14 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1656 GACCTTGTC
AvrII 99 CCTAGG C^CTAGG
1909 CCTAGG
BalI 1021 TGGCCA TGG^CCA
1230 TGGCCA
BbsI 636 GAAGAC GAAGAC(2/6)
892 GTCTTC
BbvCI 29 CCTCAGC CCTCAGC(-5/-2)
1437 CCTCAGC
BglII 845 AGATCT A^GATCT
2426 AGATCT
BlnI 99 CCTAGG C^CTAGG
1909 CCTAGG
BpmI 1628 CTCCAG CTGGAG(16/14)
1764 CTGGAG
BpuAI 636 GAAGAC GAAGAC(2/6)
892 GTCTTC
BsaHI 742 GACGCC G[GA]^CG[CT]C
1432 GACGCC
BsaWI 221 ACCGGA [AT]^CCGG[AT]
632 ACCGGA
BsgI 2068 CTGCAC GTGCAG(16/14)
2224 GTGCAG
BspHI 1523 TCATGA T^CATGA
2474 TCATGA
BspMI 2148 ACCTGC ACCTGC(4/8)
115 GCAGGT
BsrDI 1032 CATTGC GCAATG(2/0)
323 GCAATG
BstXI 1960 CCATCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2078 CCATGGAGATGG
CfoI 679 GCGC GCG^C
2468 GCGC
DsaI 1200 CCGTGG C^C[GA][CT]GG
2078 CCATGG
EcoNI 423 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2115 CCTGGCTCAGG
FauI 34 GCGGG CCCGC(4/6)
992 GCGGG
HgaI 742 GACGC GACGC(5/10)
1432 GACGC
HhaI 679 GCGC GCG^C
2468 GCGC
Hin1I 742 GACGCC G[GA]^CG[CT]C
1432 GACGCC
HinP1I 679 GCGC G^CGC
2468 GCGC
MluNI 1021 TGGCCA TGG^CCA
1230 TGGCCA
MscI 1021 TGGCCA TGG^CCA
1230 TGGCCA
MseI 629 TTAA T^TAA
2458 TTAA
PflFI 14 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1656 GACCTTGTC
RcaI 1523 TCATGA T^CATGA
2474 TCATGA
TfiI 1397 GATTC G^A[AT]TC
1624 GAATC
Tru9I 629 TTAA T^TAA
2458 TTAA
Tth111I 14 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1656 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII