################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII AccII AclI AflII AflIII AgeI AscI AseI AsnI BclI BfrI BlpI Bpu1102I BsiWI BspDI BspHI BspLU11I BssHII Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI EcoT22I FbaI FseI HpaI KspI MfeI MluI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 1989 GTCTAC GT^[AC][GT]AC AhdI 2678 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2590 AGCGCT AGC^GCT ApaI 5241 GGGCCC GGGCC^C Asp700I 1109 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2678 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 5586 CCTAGG C^CTAGG BbeI 81 GGCGCC GGCGC^C BcgI 510 GCAGGGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 5586 CCTAGG C^CTAGG BsaBI 4556 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1250 CGTCTC CGTCTC(1/5) BsmI 2100 GAATGC GAATGC(1/-1) BstEII 521 GGTGACC G^GT[ACGT]ACC BstPI 521 GGTGACC G^GT[ACGT]ACC BstXI 2788 CCAGTAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraI 5630 TTTAAA TTT^AAA DrdI 981 GACCCGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4505 CGGCCG C^GGCCG Eam1105I 2678 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 4505 CGGCCG C^GGCCG Eco47III 2590 AGCGCT AGC^GCT Eco52I 4505 CGGCCG C^GGCCG EcoO65I 521 GGTGACC G^GT[ACGT]ACC KasI 81 GGCGCC G^GCGCC MamI 4556 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 81 GGCGCC GG^CGCC PaeR7I 1525 CTCGAG C^TCGAG SbfI 2120 CCTGCAGG CCTGCA^GG ScaI 3895 AGTACT AGT^ACT SexAI 3493 ACCAGGT A^CC[AT]GGT SfoI 81 GGCGCC GGC^GCC SphI 2668 GCATGC GCATG^C Sse8387I 2120 CCTGCAGG CCTGCA^GG StuI 3043 AGGCCT AGG^CCT XbaI 2369 TCTAGA T^CTAGA XhoI 1525 CTCGAG C^TCGAG XmnI 1109 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 81 GGCGCC G[GA]^CG[CT]C 735 GACGCC Alw44I 743 GTGCAC G^TGCAC 3753 GTGCAC ApaLI 743 GTGCAC G^TGCAC 3753 GTGCAC BamHI 2955 GGATCC G^GATCC 5195 GGATCC BbrPI 61 CACGTG CAC^GTG 1139 CACGTG BglII 2812 AGATCT A^GATCT 3931 AGATCT BsaHI 81 GGCGCC G[GA]^CG[CT]C 735 GACGCC BssSI 186 CACGAG CACGAG(-5/-1) 5296 CACGAG BstAPI 57 GCATCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5541 GCAGTCTGTGC EarI 1413 CTCTTC CTCTTC(1/4) 2739 GAAGAG EcoRI 3627 GAATTC G^AATTC 5642 GAATTC EcoRV 3151 GATATC GAT^ATC 4479 GATATC Hin1I 81 GGCGCC G[GA]^CG[CT]C 735 GACGCC HincII 4863 GTCAAC GT[CT]^[GA]AC 5037 GTCAAC HindII 4863 GTCAAC GT[CT]^[GA]AC 5037 GTCAAC HindIII 1361 AAGCTT A^AGCTT 1545 AAGCTT Ksp632I 1413 CTCTTC CTCTTC(1/4) 2739 GAAGAG NspI 2668 GCATGC [GA]CATG^[CT] 4945 GCATGT PflMI 758 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5132 CCAGTGCCTGG PmaCI 61 CACGTG CAC^GTG 1139 CACGTG PmlI 61 CACGTG CAC^GTG 1139 CACGTG Van91I 758 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5132 CCAGTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### Acc65I AccIII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp718I AspHI AspI AvaI AvaII AviII BalI BanI BanII BbsI BbvCI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmaCI XmaI