##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI AscI AseI AsnI BclI
BfrI BlpI Bpu1102I BsiWI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
EcoT22I FbaI FseI HpaI KspI
MfeI MluI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1989 GTCTAC GT^[AC][GT]AC
AhdI 2678 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2590 AGCGCT AGC^GCT
ApaI 5241 GGGCCC GGGCC^C
Asp700I 1109 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2678 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 5586 CCTAGG C^CTAGG
BbeI 81 GGCGCC GGCGC^C
BcgI 510 GCAGGGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 5586 CCTAGG C^CTAGG
BsaBI 4556 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1250 CGTCTC CGTCTC(1/5)
BsmI 2100 GAATGC GAATGC(1/-1)
BstEII 521 GGTGACC G^GT[ACGT]ACC
BstPI 521 GGTGACC G^GT[ACGT]ACC
BstXI 2788 CCAGTAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 5630 TTTAAA TTT^AAA
DrdI 981 GACCCGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4505 CGGCCG C^GGCCG
Eam1105I 2678 GACTGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4505 CGGCCG C^GGCCG
Eco47III 2590 AGCGCT AGC^GCT
Eco52I 4505 CGGCCG C^GGCCG
EcoO65I 521 GGTGACC G^GT[ACGT]ACC
KasI 81 GGCGCC G^GCGCC
MamI 4556 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 81 GGCGCC GG^CGCC
PaeR7I 1525 CTCGAG C^TCGAG
SbfI 2120 CCTGCAGG CCTGCA^GG
ScaI 3895 AGTACT AGT^ACT
SexAI 3493 ACCAGGT A^CC[AT]GGT
SfoI 81 GGCGCC GGC^GCC
SphI 2668 GCATGC GCATG^C
Sse8387I 2120 CCTGCAGG CCTGCA^GG
StuI 3043 AGGCCT AGG^CCT
XbaI 2369 TCTAGA T^CTAGA
XhoI 1525 CTCGAG C^TCGAG
XmnI 1109 GAACCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 81 GGCGCC G[GA]^CG[CT]C
735 GACGCC
Alw44I 743 GTGCAC G^TGCAC
3753 GTGCAC
ApaLI 743 GTGCAC G^TGCAC
3753 GTGCAC
BamHI 2955 GGATCC G^GATCC
5195 GGATCC
BbrPI 61 CACGTG CAC^GTG
1139 CACGTG
BglII 2812 AGATCT A^GATCT
3931 AGATCT
BsaHI 81 GGCGCC G[GA]^CG[CT]C
735 GACGCC
BssSI 186 CACGAG CACGAG(-5/-1)
5296 CACGAG
BstAPI 57 GCATCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5541 GCAGTCTGTGC
EarI 1413 CTCTTC CTCTTC(1/4)
2739 GAAGAG
EcoRI 3627 GAATTC G^AATTC
5642 GAATTC
EcoRV 3151 GATATC GAT^ATC
4479 GATATC
Hin1I 81 GGCGCC G[GA]^CG[CT]C
735 GACGCC
HincII 4863 GTCAAC GT[CT]^[GA]AC
5037 GTCAAC
HindII 4863 GTCAAC GT[CT]^[GA]AC
5037 GTCAAC
HindIII 1361 AAGCTT A^AGCTT
1545 AAGCTT
Ksp632I 1413 CTCTTC CTCTTC(1/4)
2739 GAAGAG
NspI 2668 GCATGC [GA]CATG^[CT]
4945 GCATGT
PflMI 758 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5132 CCAGTGCCTGG
PmaCI 61 CACGTG CAC^GTG
1139 CACGTG
PmlI 61 CACGTG CAC^GTG
1139 CACGTG
Van91I 758 CCACTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5132 CCAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccIII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp718I AspHI AspI AvaI AvaII
AviII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII XmaCI
XmaI