##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
BbeI BcgI BglI BsiEI BsiWI
BspDI BsrFI BssHII BstEII BstPI
BstUI BstXI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV FseI
FspI HpaI KasI KspI MluI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2156 GACGTC GACGT^C
Acc65I 2661 GGTACC G^GTACC
AccIII 427 TCCGGA T^CCGGA
AclI 1706 AACGTT AA^CGTT
Aor51HI 4674 AGCGCT AGC^GCT
Asp718I 2661 GGTACC G^GTACC
AspI 106 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 5305 CACGTG CAC^GTG
BciVI 2456 GTATCC GTATCC(6/5)
BsaI 1674 GAGACC GGTCTC(1/5)
BseAI 427 TCCGGA T^CCGGA
BsmBI 2154 GAGACG CGTCTC(1/5)
Bsp1407I 4380 TGTACA T^GTACA
BspEI 427 TCCGGA T^CCGGA
BspLU11I 2473 ACATGT A^CATGT
BsrGI 4380 TGTACA T^GTACA
BssSI 1220 CTCGTG CACGAG(-5/-1)
BstAPI 1820 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2757 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4674 AGCGCT AGC^GCT
EcoT22I 1084 ATGCAT ATGCA^T
KpnI 2661 GGTACC GGTAC^C
MfeI 2656 CAATTG C^AATTG
MroI 427 TCCGGA T^CCGGA
MunI 2656 CAATTG C^AATTG
NsiI 1084 ATGCAT ATGCA^T
PflFI 106 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5305 CACGTG CAC^GTG
PmlI 5305 CACGTG CAC^GTG
PpuMI 2410 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 5561 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1706 AACGTT AA^CGTT
SacI 336 GAGCTC GAGCT^C
SexAI 2758 ACCTGGT A^CC[AT]GGT
SphI 1433 GCATGC GCATG^C
SspBI 4380 TGTACA T^GTACA
StuI 4152 AGGCCT AGG^CCT
Tth111I 106 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3127 CCAGAGTCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AocI 1290 CCTCAGG CC^T[ACGT]AGG
2237 CCTGAGG
Asp700I 915 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1146 GAATACATTC
AvaII 2411 GGACC G^G[AT]CC
3212 GGACC
AvrII 180 CCTAGG C^CTAGG
4155 CCTAGG
BalI 1129 TGGCCA TGG^CCA
2005 TGGCCA
BamHI 1163 GGATCC G^GATCC
3077 GGATCC
BanI 2661 GGTACC G^G[CT][GA]CC
2778 GGTGCC
BbvCI 2820 CCTCAGC CCTCAGC(-5/-2)
2139 GCTGAGG
BlnI 180 CCTAGG C^CTAGG
4155 CCTAGG
BlpI 1848 GCTCAGC GC^T[ACGT]AGC
5782 GCTAAGC
Bpu1102I 1848 GCTCAGC GC^T[ACGT]AGC
5782 GCTAAGC
BsaAI 1598 CACGTA [CT]AC^GT[GA]
5305 CACGTG
BsaBI 1904 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2487 GATTGCTATC
BsmI 5007 GAATGC GAATGC(1/-1)
4931 GCATTC
BspHI 1015 TCATGA T^CATGA
3085 TCATGA
BsrBI 173 CCGCTC CCGCTC(-3/-3)
419 CCGCTC
Bst1107I 1757 GTATAC GTA^TAC
4659 GTATAC
BstBI 997 TTCGAA TT^CGAA
1893 TTCGAA
BstZ17I 1757 GTATAC GTA^TAC
4659 GTATAC
Bsu36I 1290 CCTCAGG CC^T[ACGT]AGG
2237 CCTGAGG
CelII 1848 GCTCAGC GC^T[ACGT]AGC
5782 GCTAAGC
Eco81I 1290 CCTCAGG CC^T[ACGT]AGG
2237 CCTGAGG
HaeII 2131 GGCGCT [GA]GCGC^[CT]
4674 AGCGCT
HgaI 3057 GACGC GACGC(5/10)
5266 GACGC
MamI 1904 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2487 GATTGCTATC
MluNI 1129 TGGCCA TGG^CCA
2005 TGGCCA
MscI 1129 TGGCCA TGG^CCA
2005 TGGCCA
NspV 997 TTCGAA TT^CGAA
1893 TTCGAA
PflMI 1453 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3374 CCACCCCCTGG
RcaI 1015 TCATGA T^CATGA
3085 TCATGA
ScaI 3729 AGTACT AGT^ACT
5542 AGTACT
SfuI 997 TTCGAA TT^CGAA
1893 TTCGAA
SmaI 2967 CCCGGG CCC^GGG
3323 CCCGGG
SspI 3885 AATATT AAT^ATT
4175 AATATT
Van91I 1453 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3374 CCACCCCCTGG
XmaCI 2967 CCCGGG C^CCGGG
3323 CCCGGG
XmaI 2967 CCCGGG C^CCGGG
3323 CCCGGG
XmnI 915 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1146 GAATACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
ApaI ApoI AspHI AvaI BanII
BbsI BbvI BclI BcnI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII