################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AatII AccI AclI AflII AgeI AocI AscI AseI AsnI AviII AvrII BamHI BbeI BbvCI BfrI BlnI BsiEI BsiWI BsmBI BspDI Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI EclXI Eco52I Eco81I EcoNI FseI FspI HpaI KasI KspI MluI NarI NotI NruI PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SexAI SfoI SgrAI SmaI SnaBI SpeI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 3471 GGTACC G^GTACC AccIII 2373 TCCGGA T^CCGGA AcyI 348 GACGCC G[GA]^CG[CT]C AhdI 434 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 635 GTGCAC G^TGCAC ApaLI 635 GTGCAC G^TGCAC Asp718I 3471 GGTACC G^GTACC AspEI 434 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 2329 CACGTG CAC^GTG BcgI 5222 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1350 TGATCA T^GATCA BsaBI 2791 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 348 GACGCC G[GA]^CG[CT]C BsaWI 2373 TCCGGA [AT]^CCGG[AT] BseAI 2373 TCCGGA T^CCGGA Bsp1407I 1650 TGTACA T^GTACA BspEI 2373 TCCGGA T^CCGGA BsrGI 1650 TGTACA T^GTACA BssHII 4978 GCGCGC G^CGCGC BstBI 1362 TTCGAA TT^CGAA BstEII 5344 GGTAACC G^GT[ACGT]ACC BstPI 5344 GGTAACC G^GT[ACGT]ACC Eam1105I 434 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 5344 GGTAACC G^GT[ACGT]ACC FbaI 1350 TGATCA T^GATCA Hin1I 348 GACGCC G[GA]^CG[CT]C KpnI 3471 GGTACC GGTAC^C MamI 2791 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1113 CAATTG C^AATTG MroI 2373 TCCGGA T^CCGGA MunI 1113 CAATTG C^AATTG NheI 3835 GCTAGC G^CTAGC NspV 1362 TTCGAA TT^CGAA PmaCI 2329 CACGTG CAC^GTG PmlI 2329 CACGTG CAC^GTG SbfI 4028 CCTGCAGG CCTGCA^GG ScaI 2895 AGTACT AGT^ACT SfiI 3349 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1362 TTCGAA TT^CGAA Sse8387I 4028 CCTGCAGG CCTGCA^GG SspBI 1650 TGTACA T^GTACA StuI 2392 AGGCCT AGG^CCT XbaI 5159 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### ApaI 1667 GGGCCC GGGCC^C 3061 GGGCCC AspI 898 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2129 GACATGGTC BciVI 2723 GGATAC GTATCC(6/5) 3729 GGATAC BsgI 3943 CTGCAC GTGCAG(16/14) 2332 GTGCAG BspLU11I 2355 ACATGT A^CATGT 3759 ACATGT BssSI 2111 CACGAG CACGAG(-5/-1) 3494 CACGAG DrdI 2981 GACGGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4280 GACATCTATGTC EcoRI 2412 GAATTC G^AATTC 6067 GAATTC EcoRV 3442 GATATC GAT^ATC 4909 GATATC HindIII 4366 AAGCTT A^AGCTT 5054 AAGCTT NaeI 238 GCCGGC GCC^GGC 5712 GCCGGC NgoMIV 238 GCCGGC G^CCGGC 5712 GCCGGC PaeR7I 804 CTCGAG C^TCGAG 5230 CTCGAG PflFI 898 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2129 GACATGGTC SapI 3662 GAAGAGC GCTCTTC(1/4) 2734 GCTCTTC SphI 939 GCATGC GCATG^C 1611 GCATGC Tth111I 898 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2129 GACATGGTC XcmI 1828 CCACAATCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2070 CCAAAGCCCTGGTGG XhoI 804 CTCGAG C^TCGAG 5230 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI Aor51HI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI