##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AocI AscI AseI AsnI AviII
AvrII BamHI BbeI BbvCI BfrI
BlnI BsiEI BsiWI BsmBI BspDI
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI EclXI Eco52I
Eco81I EcoNI FseI FspI HpaI
KasI KspI MluI NarI NotI
NruI PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SexAI SfoI SgrAI SmaI
SnaBI SpeI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3471 GGTACC G^GTACC
AccIII 2373 TCCGGA T^CCGGA
AcyI 348 GACGCC G[GA]^CG[CT]C
AhdI 434 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 635 GTGCAC G^TGCAC
ApaLI 635 GTGCAC G^TGCAC
Asp718I 3471 GGTACC G^GTACC
AspEI 434 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2329 CACGTG CAC^GTG
BcgI 5222 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1350 TGATCA T^GATCA
BsaBI 2791 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 348 GACGCC G[GA]^CG[CT]C
BsaWI 2373 TCCGGA [AT]^CCGG[AT]
BseAI 2373 TCCGGA T^CCGGA
Bsp1407I 1650 TGTACA T^GTACA
BspEI 2373 TCCGGA T^CCGGA
BsrGI 1650 TGTACA T^GTACA
BssHII 4978 GCGCGC G^CGCGC
BstBI 1362 TTCGAA TT^CGAA
BstEII 5344 GGTAACC G^GT[ACGT]ACC
BstPI 5344 GGTAACC G^GT[ACGT]ACC
Eam1105I 434 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5344 GGTAACC G^GT[ACGT]ACC
FbaI 1350 TGATCA T^GATCA
Hin1I 348 GACGCC G[GA]^CG[CT]C
KpnI 3471 GGTACC GGTAC^C
MamI 2791 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1113 CAATTG C^AATTG
MroI 2373 TCCGGA T^CCGGA
MunI 1113 CAATTG C^AATTG
NheI 3835 GCTAGC G^CTAGC
NspV 1362 TTCGAA TT^CGAA
PmaCI 2329 CACGTG CAC^GTG
PmlI 2329 CACGTG CAC^GTG
SbfI 4028 CCTGCAGG CCTGCA^GG
ScaI 2895 AGTACT AGT^ACT
SfiI 3349 GGCCATCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1362 TTCGAA TT^CGAA
Sse8387I 4028 CCTGCAGG CCTGCA^GG
SspBI 1650 TGTACA T^GTACA
StuI 2392 AGGCCT AGG^CCT
XbaI 5159 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 1667 GGGCCC GGGCC^C
3061 GGGCCC
AspI 898 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2129 GACATGGTC
BciVI 2723 GGATAC GTATCC(6/5)
3729 GGATAC
BsgI 3943 CTGCAC GTGCAG(16/14)
2332 GTGCAG
BspLU11I 2355 ACATGT A^CATGT
3759 ACATGT
BssSI 2111 CACGAG CACGAG(-5/-1)
3494 CACGAG
DrdI 2981 GACGGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4280 GACATCTATGTC
EcoRI 2412 GAATTC G^AATTC
6067 GAATTC
EcoRV 3442 GATATC GAT^ATC
4909 GATATC
HindIII 4366 AAGCTT A^AGCTT
5054 AAGCTT
NaeI 238 GCCGGC GCC^GGC
5712 GCCGGC
NgoMIV 238 GCCGGC G^CCGGC
5712 GCCGGC
PaeR7I 804 CTCGAG C^TCGAG
5230 CTCGAG
PflFI 898 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2129 GACATGGTC
SapI 3662 GAAGAGC GCTCTTC(1/4)
2734 GCTCTTC
SphI 939 GCATGC GCATG^C
1611 GCATGC
Tth111I 898 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2129 GACATGGTC
XcmI 1828 CCACAATCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2070 CCAAAGCCCTGGTGG
XhoI 804 CTCGAG C^TCGAG
5230 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI