################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## AatII AccII AccIII AclI AgeI AocI Aor51HI ApaI AscI Asp700I AviII BcgI BclI BglI BglII BseAI BsiWI BspDI BspEI BssHII BstUI BstXI Bsu36I ClaI CpoI DrdI Eco47III Eco81I FauI FbaI FseI FspI HpaI KspI MluI MroI MvnI NaeI NgoMIV NheI NotI NruI PacI PflMI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### BamHI 1647 GGATCC G^GATCC BbsI 5667 GAAGAC GAAGAC(2/6) BciVI 5960 GTATCC GTATCC(6/5) BpuAI 5667 GAAGAC GAAGAC(2/6) BsaBI 1735 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 4433 GAGACC GGTCTC(1/5) BsaWI 6315 TCCGGT [AT]^CCGG[AT] BsiEI 1187 CGGCCG CG[GA][CT]^CG BsmBI 1254 GAGACG CGTCTC(1/5) BspHI 4769 TCATGA T^CATGA BspLU11I 4361 ACATGT A^CATGT BsrBI 1184 GAGCGG CCGCTC(-3/-3) BsrFI 5712 ACCGGC [GA]^CCGG[CT] BssSI 2468 CTCGTG CACGAG(-5/-1) Bst1107I 528 GTATAC GTA^TAC BstAPI 1236 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1386 TTCGAA TT^CGAA BstEII 3291 GGTGACC G^GT[ACGT]ACC BstPI 3291 GGTGACC G^GT[ACGT]ACC BstZ17I 528 GTATAC GTA^TAC Cfr10I 5712 ACCGGC [GA]^CCGG[CT] DraIII 5255 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1187 CGGCCG C^GGCCG EclXI 1187 CGGCCG C^GGCCG Eco52I 1187 CGGCCG C^GGCCG EcoO65I 3291 GGTGACC G^GT[ACGT]ACC HincII 462 GTTGAC GT[CT]^[GA]AC HindII 462 GTTGAC GT[CT]^[GA]AC MamI 1735 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 4275 CAGCTG C[AC]G^C[GT]G NspV 1386 TTCGAA TT^CGAA PaeR7I 1108 CTCGAG C^TCGAG PvuII 4275 CAGCTG CAG^CTG RcaI 4769 TCATGA T^CATGA SfuI 1386 TTCGAA TT^CGAA XbaI 5904 TCTAGA T^CTAGA XhoI 1108 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1712 GGTACC G^GTACC 1906 GGTACC AcyI 950 GGCGCC G[GA]^CG[CT]C 1229 GGCGCC AflII 865 CTTAAG C^TTAAG 3627 CTTAAG AflIII 1526 ACGTGT A^C[GA][CT]GT 4361 ACATGT AhdI 1256 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2890 GACAAAATGTC Alw44I 5630 GTGCAC G^TGCAC 6357 GTGCAC AlwNI 1571 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 6181 CAGCATCTG ApaLI 5630 GTGCAC G^TGCAC 6357 GTGCAC Asp718I 1712 GGTACC G^GTACC 1906 GGTACC AspEI 1256 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2890 GACAAAATGTC AspI 3138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6447 GACCCTGTC AvrII 2626 CCTAGG C^CTAGG 4292 CCTAGG BbeI 950 GGCGCC GGCGC^C 1229 GGCGCC BbrPI 1525 CACGTG CAC^GTG 5804 CACGTG BbvCI 2131 GCTGAGG CCTCAGC(-5/-2) 3369 GCTGAGG BcnI 2049 CCGGG CC^[GC]GG 5524 CCCGG BfrI 865 CTTAAG C^TTAAG 3627 CTTAAG BlnI 2626 CCTAGG C^CTAGG 4292 CCTAGG BlpI 2425 GCTAAGC GC^T[ACGT]AGC 3657 GCTCAGC Bpu1102I 2425 GCTAAGC GC^T[ACGT]AGC 3657 GCTCAGC BsaAI 1525 CACGTG [CT]AC^GT[GA] 5804 CACGTG BsaHI 950 GGCGCC G[GA]^CG[CT]C 1229 GGCGCC BsrDI 5580 CATTGC GCAATG(2/0) 4475 GCAATG BstYI 1269 AGATCC [GA]^GATC[CT] 1647 GGATCC CelII 2425 GCTAAGC GC^T[ACGT]AGC 3657 GCTCAGC Eam1105I 1256 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2890 GACAAAATGTC EcoRV 439 GATATC GAT^ATC 6063 GATATC EcoT22I 6029 ATGCAT ATGCA^T 6880 ATGCAT HgaI 1256 GACGC GACGC(5/10) 2024 GCGTC Hin1I 950 GGCGCC G[GA]^CG[CT]C 1229 GGCGCC KasI 950 GGCGCC G^GCGCC 1229 GGCGCC KpnI 1712 GGTACC GGTAC^C 1906 GGTACC MflI 1269 AGATCC [GA]^GATC[CT] 1647 GGATCC NarI 950 GGCGCC GG^CGCC 1229 GGCGCC NciI 2049 CCGGG CC^[GC]GG 5524 CCCGG NdeI 2568 CATATG CA^TATG 6693 CATATG NsiI 6029 ATGCAT ATGCA^T 6880 ATGCAT PflFI 3138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6447 GACCCTGTC PmaCI 1525 CACGTG CAC^GTG 5804 CACGTG PmlI 1525 CACGTG CAC^GTG 5804 CACGTG PshAI 2721 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3278 GACTGCAGTC SfoI 950 GGCGCC GGC^GCC 1229 GGCGCC SwaI 2820 ATTTAAAT ATTT^AAAT 6930 ATTTAAAT Tth111I 3138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 6447 GACCCTGTC XcmI 13 CCAGGAAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1273 CCATGGGGAAACTGG XhoII 1269 AGATCC [GA]^GATC[CT] 1647 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MluNI MnlI MscI MseI MslI MspI MunI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I