##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AocI Aor51HI ApaI AscI Asp700I
AviII BcgI BclI BglI BglII
BseAI BsiWI BspDI BspEI BssHII
BstUI BstXI Bsu36I ClaI CpoI
DrdI Eco47III Eco81I FauI FbaI
FseI FspI HpaI KspI MluI
MroI MvnI NaeI NgoMIV NheI
NotI NruI PacI PflMI PinAI
PmeI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I Van91I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
BamHI 1647 GGATCC G^GATCC
BbsI 5667 GAAGAC GAAGAC(2/6)
BciVI 5960 GTATCC GTATCC(6/5)
BpuAI 5667 GAAGAC GAAGAC(2/6)
BsaBI 1735 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4433 GAGACC GGTCTC(1/5)
BsaWI 6315 TCCGGT [AT]^CCGG[AT]
BsiEI 1187 CGGCCG CG[GA][CT]^CG
BsmBI 1254 GAGACG CGTCTC(1/5)
BspHI 4769 TCATGA T^CATGA
BspLU11I 4361 ACATGT A^CATGT
BsrBI 1184 GAGCGG CCGCTC(-3/-3)
BsrFI 5712 ACCGGC [GA]^CCGG[CT]
BssSI 2468 CTCGTG CACGAG(-5/-1)
Bst1107I 528 GTATAC GTA^TAC
BstAPI 1236 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1386 TTCGAA TT^CGAA
BstEII 3291 GGTGACC G^GT[ACGT]ACC
BstPI 3291 GGTGACC G^GT[ACGT]ACC
BstZ17I 528 GTATAC GTA^TAC
Cfr10I 5712 ACCGGC [GA]^CCGG[CT]
DraIII 5255 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1187 CGGCCG C^GGCCG
EclXI 1187 CGGCCG C^GGCCG
Eco52I 1187 CGGCCG C^GGCCG
EcoO65I 3291 GGTGACC G^GT[ACGT]ACC
HincII 462 GTTGAC GT[CT]^[GA]AC
HindII 462 GTTGAC GT[CT]^[GA]AC
MamI 1735 GATCCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 4275 CAGCTG C[AC]G^C[GT]G
NspV 1386 TTCGAA TT^CGAA
PaeR7I 1108 CTCGAG C^TCGAG
PvuII 4275 CAGCTG CAG^CTG
RcaI 4769 TCATGA T^CATGA
SfuI 1386 TTCGAA TT^CGAA
XbaI 5904 TCTAGA T^CTAGA
XhoI 1108 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1712 GGTACC G^GTACC
1906 GGTACC
AcyI 950 GGCGCC G[GA]^CG[CT]C
1229 GGCGCC
AflII 865 CTTAAG C^TTAAG
3627 CTTAAG
AflIII 1526 ACGTGT A^C[GA][CT]GT
4361 ACATGT
AhdI 1256 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2890 GACAAAATGTC
Alw44I 5630 GTGCAC G^TGCAC
6357 GTGCAC
AlwNI 1571 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
6181 CAGCATCTG
ApaLI 5630 GTGCAC G^TGCAC
6357 GTGCAC
Asp718I 1712 GGTACC G^GTACC
1906 GGTACC
AspEI 1256 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2890 GACAAAATGTC
AspI 3138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6447 GACCCTGTC
AvrII 2626 CCTAGG C^CTAGG
4292 CCTAGG
BbeI 950 GGCGCC GGCGC^C
1229 GGCGCC
BbrPI 1525 CACGTG CAC^GTG
5804 CACGTG
BbvCI 2131 GCTGAGG CCTCAGC(-5/-2)
3369 GCTGAGG
BcnI 2049 CCGGG CC^[GC]GG
5524 CCCGG
BfrI 865 CTTAAG C^TTAAG
3627 CTTAAG
BlnI 2626 CCTAGG C^CTAGG
4292 CCTAGG
BlpI 2425 GCTAAGC GC^T[ACGT]AGC
3657 GCTCAGC
Bpu1102I 2425 GCTAAGC GC^T[ACGT]AGC
3657 GCTCAGC
BsaAI 1525 CACGTG [CT]AC^GT[GA]
5804 CACGTG
BsaHI 950 GGCGCC G[GA]^CG[CT]C
1229 GGCGCC
BsrDI 5580 CATTGC GCAATG(2/0)
4475 GCAATG
BstYI 1269 AGATCC [GA]^GATC[CT]
1647 GGATCC
CelII 2425 GCTAAGC GC^T[ACGT]AGC
3657 GCTCAGC
Eam1105I 1256 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2890 GACAAAATGTC
EcoRV 439 GATATC GAT^ATC
6063 GATATC
EcoT22I 6029 ATGCAT ATGCA^T
6880 ATGCAT
HgaI 1256 GACGC GACGC(5/10)
2024 GCGTC
Hin1I 950 GGCGCC G[GA]^CG[CT]C
1229 GGCGCC
KasI 950 GGCGCC G^GCGCC
1229 GGCGCC
KpnI 1712 GGTACC GGTAC^C
1906 GGTACC
MflI 1269 AGATCC [GA]^GATC[CT]
1647 GGATCC
NarI 950 GGCGCC GG^CGCC
1229 GGCGCC
NciI 2049 CCGGG CC^[GC]GG
5524 CCCGG
NdeI 2568 CATATG CA^TATG
6693 CATATG
NsiI 6029 ATGCAT ATGCA^T
6880 ATGCAT
PflFI 3138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6447 GACCCTGTC
PmaCI 1525 CACGTG CAC^GTG
5804 CACGTG
PmlI 1525 CACGTG CAC^GTG
5804 CACGTG
PshAI 2721 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3278 GACTGCAGTC
SfoI 950 GGCGCC GGC^GCC
1229 GGCGCC
SwaI 2820 ATTTAAAT ATTT^AAAT
6930 ATTTAAAT
Tth111I 3138 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6447 GACCCTGTC
XcmI 13 CCAGGAAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1273 CCATGGGGAAACTGG
XhoII 1269 AGATCC [GA]^GATC[CT]
1647 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaI AvaII BalI BanI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MluNI MnlI MscI MseI
MslI MspI MunI MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PstI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I