##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII Acc65I AflII AhdI AscI
AseI AsnI Asp718I AspEI AvrII
BfrI BlnI BspDI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI Eam1105I
EcoO65I FseI KpnI MfeI MunI
NdeI NheI NruI NspV PacI
PmeI PshAI PshBI PvuI SalI
SfuI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 5507 TCCGGA T^CCGGA
AclI 5779 AACGTT AA^CGTT
AgeI 3471 ACCGGT A^CCGGT
AocI 4467 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2599 AGCGCT AGC^GCT
BamHI 332 GGATCC G^GATCC
BciVI 3917 GTATCC GTATCC(6/5)
BglII 938 AGATCT A^GATCT
BseAI 5507 TCCGGA T^CCGGA
BsiEI 212 CGGCCG CG[GA][CT]^CG
BsiWI 1313 CGTACG C^GTACG
BsmI 3880 GAATGC GAATGC(1/-1)
BspEI 5507 TCCGGA T^CCGGA
BspLU11I 5074 ACATGT A^CATGT
BssHII 1606 GCGCGC G^CGCGC
BstXI 1143 CCACAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4467 CCTCAGG CC^T[ACGT]AGG
CpoI 3887 CGGTCCG CG^G[AT]CCG
EagI 212 CGGCCG C^GGCCG
EclXI 212 CGGCCG C^GGCCG
Eco47III 2599 AGCGCT AGC^GCT
Eco52I 212 CGGCCG C^GGCCG
Eco81I 4467 CCTCAGG CC^T[ACGT]AGG
EcoRV 1624 GATATC GAT^ATC
EcoT22I 2346 ATGCAT ATGCA^T
HindIII 5502 AAGCTT A^AGCTT
HpaI 6397 GTTAAC GTT^AAC
MluI 1310 ACGCGT A^CGCGT
MroI 5507 TCCGGA T^CCGGA
NotI 211 GCGGCCGC GC^GGCCGC
NsiI 2346 ATGCAT ATGCA^T
PaeR7I 2401 CTCGAG C^TCGAG
PinAI 3471 ACCGGT A^CCGGT
Psp1406I 5779 AACGTT AA^CGTT
RsrII 3887 CGGTCCG CG^G[AT]CCG
SgrAI 1390 CGCCGGCG C[GA]^CCGG[CT]G
XhoI 2401 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1310 ACGCGT A^C[GA][CT]GT
5074 ACATGT
Asp700I 495 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4725 GAAGACCTTC
BbrPI 2686 CACGTG CAC^GTG
6183 CACGTG
BbvCI 5994 CCTCAGC CCTCAGC(-5/-2)
6406 GCTGAGG
BcgI 5146 CGAGGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3062 GCATCCGCTTCG
BsaBI 3906 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3912 GATCAGTATC
BsmBI 195 CGTCTC CGTCTC(1/5)
4785 CGTCTC
Bsp1407I 1070 TGTACA T^GTACA
6465 TGTACA
BsrGI 1070 TGTACA T^GTACA
6465 TGTACA
BstAPI 4227 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4476 GCACACGGTGC
DraI 5807 TTTAAA TTT^AAA
6459 TTTAAA
DrdI 1309 GACGCGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1710 GACCTACAAGTC
EcoRI 1852 GAATTC G^AATTC
4341 GAATTC
MamI 3906 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3912 GATCAGTATC
NaeI 1391 GCCGGC GCC^GGC
1655 GCCGGC
NcoI 3122 CCATGG C^CATGG
3152 CCATGG
NgoMIV 1391 GCCGGC G^CCGGC
1655 GCCGGC
PmaCI 2686 CACGTG CAC^GTG
6183 CACGTG
PmlI 2686 CACGTG CAC^GTG
6183 CACGTG
SbfI 777 CCTGCAGG CCTGCA^GG
2082 CCTGCAGG
SfiI 2205 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6425 GGCCCCGAGGGCC
Sse8387I 777 CCTGCAGG CCTGCA^GG
2082 CCTGCAGG
SspBI 1070 TGTACA T^GTACA
6465 TGTACA
XmnI 495 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4725 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII AviII BalI BanI
BanII BbeI BbsI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI SacII SapI Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SfoI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI