################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AatII Acc65I AflII AhdI AscI AseI AsnI Asp718I AspEI AvrII BfrI BlnI BspDI Bst1107I BstBI BstEII BstPI BstZ17I ClaI Eam1105I EcoO65I FseI KpnI MfeI MunI NdeI NheI NruI NspV PacI PmeI PshAI PshBI PvuI SalI SfuI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccIII 5507 TCCGGA T^CCGGA AclI 5779 AACGTT AA^CGTT AgeI 3471 ACCGGT A^CCGGT AocI 4467 CCTCAGG CC^T[ACGT]AGG Aor51HI 2599 AGCGCT AGC^GCT BamHI 332 GGATCC G^GATCC BciVI 3917 GTATCC GTATCC(6/5) BglII 938 AGATCT A^GATCT BseAI 5507 TCCGGA T^CCGGA BsiEI 212 CGGCCG CG[GA][CT]^CG BsiWI 1313 CGTACG C^GTACG BsmI 3880 GAATGC GAATGC(1/-1) BspEI 5507 TCCGGA T^CCGGA BspLU11I 5074 ACATGT A^CATGT BssHII 1606 GCGCGC G^CGCGC BstXI 1143 CCACAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 4467 CCTCAGG CC^T[ACGT]AGG CpoI 3887 CGGTCCG CG^G[AT]CCG EagI 212 CGGCCG C^GGCCG EclXI 212 CGGCCG C^GGCCG Eco47III 2599 AGCGCT AGC^GCT Eco52I 212 CGGCCG C^GGCCG Eco81I 4467 CCTCAGG CC^T[ACGT]AGG EcoRV 1624 GATATC GAT^ATC EcoT22I 2346 ATGCAT ATGCA^T HindIII 5502 AAGCTT A^AGCTT HpaI 6397 GTTAAC GTT^AAC MluI 1310 ACGCGT A^CGCGT MroI 5507 TCCGGA T^CCGGA NotI 211 GCGGCCGC GC^GGCCGC NsiI 2346 ATGCAT ATGCA^T PaeR7I 2401 CTCGAG C^TCGAG PinAI 3471 ACCGGT A^CCGGT Psp1406I 5779 AACGTT AA^CGTT RsrII 3887 CGGTCCG CG^G[AT]CCG SgrAI 1390 CGCCGGCG C[GA]^CCGG[CT]G XhoI 2401 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 1310 ACGCGT A^C[GA][CT]GT 5074 ACATGT Asp700I 495 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4725 GAAGACCTTC BbrPI 2686 CACGTG CAC^GTG 6183 CACGTG BbvCI 5994 CCTCAGC CCTCAGC(-5/-2) 6406 GCTGAGG BcgI 5146 CGAGGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3062 GCATCCGCTTCG BsaBI 3906 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3912 GATCAGTATC BsmBI 195 CGTCTC CGTCTC(1/5) 4785 CGTCTC Bsp1407I 1070 TGTACA T^GTACA 6465 TGTACA BsrGI 1070 TGTACA T^GTACA 6465 TGTACA BstAPI 4227 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4476 GCACACGGTGC DraI 5807 TTTAAA TTT^AAA 6459 TTTAAA DrdI 1309 GACGCGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1710 GACCTACAAGTC EcoRI 1852 GAATTC G^AATTC 4341 GAATTC MamI 3906 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3912 GATCAGTATC NaeI 1391 GCCGGC GCC^GGC 1655 GCCGGC NcoI 3122 CCATGG C^CATGG 3152 CCATGG NgoMIV 1391 GCCGGC G^CCGGC 1655 GCCGGC PmaCI 2686 CACGTG CAC^GTG 6183 CACGTG PmlI 2686 CACGTG CAC^GTG 6183 CACGTG SbfI 777 CCTGCAGG CCTGCA^GG 2082 CCTGCAGG SfiI 2205 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 6425 GGCCCCGAGGGCC Sse8387I 777 CCTGCAGG CCTGCA^GG 2082 CCTGCAGG SspBI 1070 TGTACA T^GTACA 6465 TGTACA XmnI 495 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4725 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 156 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbeI BbsI BbvI BclI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SacII SapI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SfoI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI