##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AgeI AscI AseI
AsnI AvrII BbeI BbrPI BbvCI
BcgI BclI BlnI BseAI BsiEI
BsiWI BspDI BspEI Bst1107I BstBI
BstZ17I ClaI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI KasI KspI MluI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI PvuI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SspI
StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 4072 GTCTAC GT^[AC][GT]AC
AclI 4590 AACGTT AA^CGTT
AcyI 3305 GGCGTC G[GA]^CG[CT]C
AlwNI 4921 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 5499 AGCGCT AGC^GCT
ApaI 6042 GGGCCC GGGCC^C
Asp700I 2247 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4795 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3593 TGCGCA TGC^GCA
BamHI 33 GGATCC G^GATCC
BsaBI 3548 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3305 GGCGTC G[GA]^CG[CT]C
BsaI 3744 GAGACC GGTCTC(1/5)
BsaWI 1315 TCCGGT [AT]^CCGG[AT]
BsgI 1261 GTGCAG GTGCAG(16/14)
BsmBI 3968 CGTCTC CGTCTC(1/5)
BspHI 612 TCATGA T^CATGA
BsrFI 1160 GCCGGC [GA]^CCGG[CT]
BssSI 5975 CTCGTG CACGAG(-5/-1)
BstEII 5896 GGTTACC G^GT[ACGT]ACC
BstPI 5896 GGTTACC G^GT[ACGT]ACC
Cfr10I 1160 GCCGGC [GA]^CCGG[CT]
CpoI 809 CGGTCCG CG^G[AT]CCG
DraIII 4871 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 5499 AGCGCT AGC^GCT
EcoNI 5274 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5896 GGTTACC G^GT[ACGT]ACC
FspI 3593 TGCGCA TGC^GCA
Hin1I 3305 GGCGTC G[GA]^CG[CT]C
HpaI 720 GTTAAC GTT^AAC
MamI 3548 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 946 CAATTG C^AATTG
MunI 946 CAATTG C^AATTG
NaeI 1160 GCCGGC GCC^GGC
NgoMIV 1160 GCCGGC G^CCGGC
PaeR7I 16 CTCGAG C^TCGAG
PflFI 4795 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4590 AACGTT AA^CGTT
RcaI 612 TCATGA T^CATGA
RsrII 809 CGGTCCG CG^G[AT]CCG
SbfI 5583 CCTGCAGG CCTGCA^GG
SpeI 5133 ACTAGT A^CTAGT
Sse8387I 5583 CCTGCAGG CCTGCA^GG
Tth111I 4795 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 16 CTCGAG C^TCGAG
XmnI 2247 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 3799 AAATTT [GA]^AATT[CT]
6321 GAATTT
Alw44I 1522 GTGCAC G^TGCAC
4877 GTGCAC
ApaLI 1522 GTGCAC G^TGCAC
4877 GTGCAC
ApoI 3799 AAATTT [GA]^AATT[CT]
6321 GAATTT
BciVI 3149 GGATAC GTATCC(6/5)
5089 GGATAC
BglI 4037 GCCCCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4570 GCCTCCCTGGC
BsaAI 775 CACGTA [CT]AC^GT[GA]
6149 CACGTA
BsmI 3856 GAATGC GAATGC(1/-1)
2137 GCATTC
Bsp1407I 1074 TGTACA T^GTACA
4392 TGTACA
BsrGI 1074 TGTACA T^GTACA
4392 TGTACA
HaeII 2098 GGCGCT [GA]GCGC^[CT]
5499 AGCGCT
NcoI 3145 CCATGG C^CATGG
3868 CCATGG
PshAI 575 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3576 GACCTTGGTC
SacI 480 GAGCTC GAGCT^C
3425 GAGCTC
SapI 1137 GCTCTTC GCTCTTC(1/4)
3197 GCTCTTC
SphI 1386 GCATGC GCATG^C
1944 GCATGC
SspBI 1074 TGTACA T^GTACA
4392 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
Acc65I AccII AciI AfaI AflII
AflIII AhdI AluI AlwI AocI
Asp718I AspEI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BfrI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII