################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrDI BssHII BssSI BstAPI BstBI BstUI Bsu36I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MspA1I MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 317 GTATAC GT^[AC][GT]AC Aor51HI 1631 AGCGCT AGC^GCT Asp700I 1306 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 1345 GGATCC G^GATCC BcgI 1610 CGAAGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1807 AGATCT A^GATCT BsaBI 29 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 85 GGTCTC GGTCTC(1/5) BseRI 1331 GAGGAG GAGGAG(10/8) BsgI 1494 GTGCAG GTGCAG(16/14) BsmBI 586 GAGACG CGTCTC(1/5) BspHI 1127 TCATGA T^CATGA Bst1107I 317 GTATAC GTA^TAC BstEII 490 GGTGACC G^GT[ACGT]ACC BstPI 490 GGTGACC G^GT[ACGT]ACC BstXI 173 CCATCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 317 GTATAC GTA^TAC DraI 1461 TTTAAA TTT^AAA DsaI 108 CCACGG C^C[GA][CT]GG EarI 483 GAAGAG CTCTTC(1/4) Eco47III 1631 AGCGCT AGC^GCT EcoO65I 490 GGTGACC G^GT[ACGT]ACC HaeII 1631 AGCGCT [GA]GCGC^[CT] Ksp632I 483 GAAGAG CTCTTC(1/4) MamI 29 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspI 1750 GCATGT [GA]CATG^[CT] PpuMI 1370 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 1127 TCATGA T^CATGA SfiI 498 GGCCAGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 164 CCCGGG CCC^GGG SmlI 1282 CTCAAG C^T[CT][GA]AG SspI 1457 AATATT AAT^ATT SwaI 1460 ATTTAAAT ATTT^AAAT TaqI 1609 TCGA T^CGA TthHB8I 1609 TCGA T^CGA XmaCI 164 CCCGGG C^CCGGG XmaI 164 CCCGGG C^CCGGG XmnI 1306 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 1264 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1406 CAGATTCTG AspHI 905 GAGCTC G[AT]GC[AT]^C 1920 GAGCTC AvaI 164 CCCGGG C^[CT]CG[GA]G 526 CCCGAG BalI 785 TGGCCA TGG^CCA 1364 TGGCCA BclI 5 TGATCA T^GATCA 2008 TGATCA BsiHKAI 905 GAGCTC G[AT]GC[AT]^C 1920 GAGCTC BsmI 1863 GCATTC GAATGC(1/-1) 1962 GCATTC BsoBI 164 CCCGGG C^[CT]CG[GA]G 526 CCCGAG BspMI 226 ACCTGC ACCTGC(4/8) 42 GCAGGT BsrBI 1598 CCGCTC CCGCTC(-3/-3) 802 GAGCGG BsrFI 635 GCCGGC [GA]^CCGG[CT] 925 GCCGGC CfoI 68 GCGC GCG^C 1632 GCGC Cfr10I 635 GCCGGC [GA]^CCGG[CT] 925 GCCGGC FbaI 5 TGATCA T^GATCA 2008 TGATCA HgaI 853 GACGC GACGC(5/10) 1105 GCGTC HhaI 68 GCGC GCG^C 1632 GCGC HinP1I 68 GCGC G^CGC 1632 GCGC MaeII 569 ACGT A^CGT 1066 ACGT MluNI 785 TGGCCA TGG^CCA 1364 TGGCCA MscI 785 TGGCCA TGG^CCA 1364 TGGCCA MslI 1512 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1953 CACACCTGTG NaeI 635 GCCGGC GCC^GGC 925 GCCGGC NgoMIV 635 GCCGGC G^CCGGC 925 GCCGGC SacI 905 GAGCTC GAGCT^C 1920 GAGCTC SexAI 868 ACCAGGT A^CC[AT]GGT 1814 ACCTGGT SfaNI 1015 GCATC GCATC(5/9) 1929 GCATC StuI 975 AGGCCT AGG^CCT 1161 AGGCCT TfiI 1002 GAATC G^A[AT]TC 1408 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SspBI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII