##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbvCI BciVI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII BssSI
BstAPI BstBI BstUI Bsu36I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MspA1I MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SfcI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I Tth111I Van91I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 317 GTATAC GT^[AC][GT]AC
Aor51HI 1631 AGCGCT AGC^GCT
Asp700I 1306 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1345 GGATCC G^GATCC
BcgI 1610 CGAAGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1807 AGATCT A^GATCT
BsaBI 29 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 85 GGTCTC GGTCTC(1/5)
BseRI 1331 GAGGAG GAGGAG(10/8)
BsgI 1494 GTGCAG GTGCAG(16/14)
BsmBI 586 GAGACG CGTCTC(1/5)
BspHI 1127 TCATGA T^CATGA
Bst1107I 317 GTATAC GTA^TAC
BstEII 490 GGTGACC G^GT[ACGT]ACC
BstPI 490 GGTGACC G^GT[ACGT]ACC
BstXI 173 CCATCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 317 GTATAC GTA^TAC
DraI 1461 TTTAAA TTT^AAA
DsaI 108 CCACGG C^C[GA][CT]GG
EarI 483 GAAGAG CTCTTC(1/4)
Eco47III 1631 AGCGCT AGC^GCT
EcoO65I 490 GGTGACC G^GT[ACGT]ACC
HaeII 1631 AGCGCT [GA]GCGC^[CT]
Ksp632I 483 GAAGAG CTCTTC(1/4)
MamI 29 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 1750 GCATGT [GA]CATG^[CT]
PpuMI 1370 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1127 TCATGA T^CATGA
SfiI 498 GGCCAGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 164 CCCGGG CCC^GGG
SmlI 1282 CTCAAG C^T[CT][GA]AG
SspI 1457 AATATT AAT^ATT
SwaI 1460 ATTTAAAT ATTT^AAAT
TaqI 1609 TCGA T^CGA
TthHB8I 1609 TCGA T^CGA
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
XmnI 1306 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 1264 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1406 CAGATTCTG
AspHI 905 GAGCTC G[AT]GC[AT]^C
1920 GAGCTC
AvaI 164 CCCGGG C^[CT]CG[GA]G
526 CCCGAG
BalI 785 TGGCCA TGG^CCA
1364 TGGCCA
BclI 5 TGATCA T^GATCA
2008 TGATCA
BsiHKAI 905 GAGCTC G[AT]GC[AT]^C
1920 GAGCTC
BsmI 1863 GCATTC GAATGC(1/-1)
1962 GCATTC
BsoBI 164 CCCGGG C^[CT]CG[GA]G
526 CCCGAG
BspMI 226 ACCTGC ACCTGC(4/8)
42 GCAGGT
BsrBI 1598 CCGCTC CCGCTC(-3/-3)
802 GAGCGG
BsrFI 635 GCCGGC [GA]^CCGG[CT]
925 GCCGGC
CfoI 68 GCGC GCG^C
1632 GCGC
Cfr10I 635 GCCGGC [GA]^CCGG[CT]
925 GCCGGC
FbaI 5 TGATCA T^GATCA
2008 TGATCA
HgaI 853 GACGC GACGC(5/10)
1105 GCGTC
HhaI 68 GCGC GCG^C
1632 GCGC
HinP1I 68 GCGC G^CGC
1632 GCGC
MaeII 569 ACGT A^CGT
1066 ACGT
MluNI 785 TGGCCA TGG^CCA
1364 TGGCCA
MscI 785 TGGCCA TGG^CCA
1364 TGGCCA
MslI 1512 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1953 CACACCTGTG
NaeI 635 GCCGGC GCC^GGC
925 GCCGGC
NgoMIV 635 GCCGGC G^CCGGC
925 GCCGGC
SacI 905 GAGCTC GAGCT^C
1920 GAGCTC
SexAI 868 ACCAGGT A^CC[AT]GGT
1814 ACCTGGT
SfaNI 1015 GCATC GCATC(5/9)
1929 GCATC
StuI 975 AGGCCT AGG^CCT
1161 AGGCCT
TfiI 1002 GAATC G^A[AT]TC
1408 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SspBI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII