##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BcgI BciVI BcnI BglI BglII
BlnI BsaWI BseAI BsiWI BspDI
BspEI BspMI BsrDI BsrFI BssHII
Bst1107I BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HpaI
KasI KpnI KspI MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 1036 GACGCC G[GA]^CG[CT]C
AflII 2030 CTTAAG C^TTAAG
ApaI 448 GGGCCC GGGCC^C
AvaI 667 CTCGAG C^[CT]CG[GA]G
BalI 251 TGGCCA TGG^CCA
BanI 163 GGTGCC G^G[CT][GA]CC
BbrPI 2302 CACGTG CAC^GTG
BbvCI 1264 GCTGAGG CCTCAGC(-5/-2)
BfrI 2030 CTTAAG C^TTAAG
BsaAI 2302 CACGTG [CT]AC^GT[GA]
BsaBI 2021 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1036 GACGCC G[GA]^CG[CT]C
BsaI 962 GAGACC GGTCTC(1/5)
BsgI 539 CTGCAC GTGCAG(16/14)
BsmBI 1150 GAGACG CGTCTC(1/5)
BsoBI 667 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1940 TGTACA T^GTACA
BspLU11I 974 ACATGT A^CATGT
BsrBI 1027 GAGCGG CCGCTC(-3/-3)
BsrGI 1940 TGTACA T^GTACA
BstAPI 1299 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1478 TTCGAA TT^CGAA
CfoI 1302 GCGC GCG^C
EaeI 251 TGGCCA [CT]^GGCC[GA]
EcoNI 1704 CCTGGACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1301 AGCGCC [GA]GCGC^[CT]
HapII 289 CCGG C^CGG
HgaI 1036 GACGC GACGC(5/10)
HhaI 1302 GCGC GCG^C
Hin1I 1036 GACGCC G[GA]^CG[CT]C
HinP1I 1302 GCGC G^CGC
HpaII 289 CCGG C^CGG
MaeII 2303 ACGT A^CGT
MamI 2021 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 251 TGGCCA TGG^CCA
MscI 251 TGGCCA TGG^CCA
MspI 289 CCGG C^CGG
NcoI 623 CCATGG C^CATGG
NspV 1478 TTCGAA TT^CGAA
PaeR7I 667 CTCGAG C^TCGAG
PflMI 2355 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2302 CACGTG CAC^GTG
PmlI 2302 CACGTG CAC^GTG
PvuII 179 CAGCTG CAG^CTG
SfuI 1478 TTCGAA TT^CGAA
SphI 2232 GCATGC GCATG^C
SspBI 1940 TGTACA T^GTACA
SspI 1597 AATATT AAT^ATT
Van91I 2355 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1818 TCTAGA T^CTAGA
XhoI 667 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1102 AAATTC [GA]^AATT[CT]
1823 AAATTT
Alw44I 263 GTGCAC G^TGCAC
382 GTGCAC
ApaLI 263 GTGCAC G^TGCAC
382 GTGCAC
ApoI 1102 AAATTC [GA]^AATT[CT]
1823 AAATTT
AspI 1781 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2121 GACTAAGTC
BbsI 1156 GAAGAC GAAGAC(2/6)
1979 GTCTTC
BlpI 67 GCTGAGC GC^T[ACGT]AGC
1189 GCTAAGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
1189 GCTAAGC
BpuAI 1156 GAAGAC GAAGAC(2/6)
1979 GTCTTC
BsiEI 641 CGACCG CG[GA][CT]^CG
1273 CGACCG
BsmI 1437 GAATGC GAATGC(1/-1)
1666 GAATGC
BssSI 385 CACGAG CACGAG(-5/-1)
2333 CTCGTG
CelII 67 GCTGAGC GC^T[ACGT]AGC
1189 GCTAAGC
DraI 295 TTTAAA TTT^AAA
2342 TTTAAA
DrdI 16 GACAAGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACTTTGGTGTC
EcoT14I 623 CCATGG C^C[AT][AT]GG
2048 CCAAGG
HincII 782 GTCAAC GT[CT]^[GA]AC
1693 GTCAAC
HindII 782 GTCAAC GT[CT]^[GA]AC
1693 GTCAAC
HindIII 464 AAGCTT A^AGCTT
1855 AAGCTT
MspA1I 179 CAGCTG C[AC]G^C[GT]G
1328 CAGCGG
NdeI 1094 CATATG CA^TATG
1107 CATATG
PflFI 1781 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2121 GACTAAGTC
PstI 268 CTGCAG CTGCA^G
1306 CTGCAG
SacI 70 GAGCTC GAGCT^C
1934 GAGCTC
SapI 1216 GAAGAGC GCTCTTC(1/4)
2076 GCTCTTC
StyI 623 CCATGG C^C[AT][AT]GG
2048 CCAAGG
Tth111I 1781 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2121 GACTAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BclI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DraIII DsaI EarI
EcoO109I EcoRII FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI