##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI BamHI BbrPI
BcgI BciVI BclI BsaAI BseAI
BsiWI BspDI BspEI BssHII BssSI
BstEII BstPI ClaI CpoI Eam1105I
Eco47III EcoO65I EcoRV FbaI FseI
HpaI KpnI MfeI MluI MroI
MunI NdeI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SalI
SbfI SfiI SnaBI SpeI Sse8387I
Tth111I
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2758 GACGTC GACGT^C
AflII 6305 CTTAAG C^TTAAG
Asp700I 236 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 79 TGCGCA TGC^GCA
AvrII 1585 CCTAGG C^CTAGG
BalI 5431 TGGCCA TGG^CCA
BbvCI 2895 CCTCAGC CCTCAGC(-5/-2)
BfrI 6305 CTTAAG C^TTAAG
BglII 5351 AGATCT A^GATCT
BlnI 1585 CCTAGG C^CTAGG
BsaWI 376 ACCGGA [AT]^CCGG[AT]
BspHI 3109 TCATGA T^CATGA
BspLU11I 4366 ACATGT A^CATGT
BspMI 4126 GCAGGT ACCTGC(4/8)
Bst1107I 4982 GTATAC GTA^TAC
BstBI 3713 TTCGAA TT^CGAA
BstZ17I 4982 GTATAC GTA^TAC
DraIII 458 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 6082 GAATTC G^AATTC
EcoT22I 5541 ATGCAT ATGCA^T
FspI 79 TGCGCA TGC^GCA
HindIII 6377 AAGCTT A^AGCTT
KspI 70 CCGCGG CCGC^GG
MluNI 5431 TGGCCA TGG^CCA
MscI 5431 TGGCCA TGG^CCA
NsiI 5541 ATGCAT ATGCA^T
NspV 3713 TTCGAA TT^CGAA
PaeR7I 5250 CTCGAG C^TCGAG
RcaI 3109 TCATGA T^CATGA
SacII 70 CCGCGG CCGC^GG
SexAI 2145 ACCAGGT A^CC[AT]GGT
SfuI 3713 TTCGAA TT^CGAA
SgrAI 108 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2397 CCCGGG CCC^GGG
StuI 584 AGGCCT AGG^CCT
XhoI 5250 CTCGAG C^TCGAG
XmaCI 2397 CCCGGG C^CCGGG
XmaI 2397 CCCGGG C^CCGGG
XmnI 236 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 4982 GTATAC GT^[AC][GT]AC
5688 GTAGAC
AccII 71 CGCG CG^CG
982 CGCG
AflIII 4366 ACATGT A^C[GA][CT]GT
5979 ACGTGT
AlwNI 725 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3772 CAGAAGCTG
AocI 1949 CCTCAGG CC^T[ACGT]AGG
4066 CCTCAGG
AvaI 2397 CCCGGG C^[CT]CG[GA]G
5250 CTCGAG
BglI 2549 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3265 GCCCGAAGGGC
BlpI 409 GCTCAGC GC^T[ACGT]AGC
5843 GCTTAGC
Bpu1102I 409 GCTCAGC GC^T[ACGT]AGC
5843 GCTTAGC
BsaBI 1413 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5800 GATTTCTATC
BsiEI 6 CGGCCG CG[GA][CT]^CG
131 CGGCCG
BsoBI 2397 CCCGGG C^[CT]CG[GA]G
5250 CTCGAG
BsrBI 5409 CCGCTC CCGCTC(-3/-3)
44 GAGCGG
BsrDI 4927 CATTGC GCAATG(2/0)
1625 GCAATG
BstUI 71 CGCG CG^CG
982 CGCG
BstXI 1653 CCAGGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2850 CCAGCCTGGTGG
Bsu36I 1949 CCTCAGG CC^T[ACGT]AGG
4066 CCTCAGG
CelII 409 GCTCAGC GC^T[ACGT]AGC
5843 GCTTAGC
DrdI 2041 GACACCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4604 GACGGGAGAGTC
EagI 6 CGGCCG C^GGCCG
131 CGGCCG
EclXI 6 CGGCCG C^GGCCG
131 CGGCCG
Eco52I 6 CGGCCG C^GGCCG
131 CGGCCG
Eco81I 1949 CCTCAGG CC^T[ACGT]AGG
4066 CCTCAGG
MamI 1413 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5800 GATTTCTATC
MvnI 71 CGCG CG^CG
982 CGCG
NheI 1597 GCTAGC G^CTAGC
4194 GCTAGC
PflMI 468 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6053 CCAGTGTTTGG
SacI 2534 GAGCTC GAGCT^C
5239 GAGCTC
SapI 860 GAAGAGC GCTCTTC(1/4)
3788 GAAGAGC
ScaI 1174 AGTACT AGT^ACT
3898 AGTACT
SwaI 6093 ATTTAAAT ATTT^AAAT
6391 ATTTAAAT
Van91I 468 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6053 CCAGTGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI SphI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII