##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI ApaI
AscI Asp718I AspI AvrII BamHI
BbeI BlnI BsiEI BsiWI BsmBI
BspDI BsrBI BssHII Bst1107I BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SgrAI SmaI SnaBI SwaI
Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2781 GACGTC GACGT^C
AccIII 1990 TCCGGA T^CCGGA
AflII 1070 CTTAAG C^TTAAG
Alw44I 6225 GTGCAC G^TGCAC
ApaLI 6225 GTGCAC G^TGCAC
AseI 4027 ATTAAT AT^TAAT
AsnI 4027 ATTAAT AT^TAAT
AviII 3144 TGCGCA TGC^GCA
BalI 6373 TGGCCA TGG^CCA
BanI 3803 GGTGCC G^G[CT][GA]CC
BcgI 2590 GCAGAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 969 GTATCC GTATCC(6/5)
BfrI 1070 CTTAAG C^TTAAG
BsaBI 2597 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1990 TCCGGA [AT]^CCGG[AT]
BseAI 1990 TCCGGA T^CCGGA
BspEI 1990 TCCGGA T^CCGGA
BspLU11I 4960 ACATGT A^CATGT
BssSI 2733 CTCGTG CACGAG(-5/-1)
BstBI 327 TTCGAA TT^CGAA
BstEII 1654 GGTCACC G^GT[ACGT]ACC
BstPI 1654 GGTCACC G^GT[ACGT]ACC
BstXI 5051 CCAAATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 1654 GGTCACC G^GT[ACGT]ACC
FspI 3144 TGCGCA TGC^GCA
MamI 2597 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 6373 TGGCCA TGG^CCA
MroI 1990 TCCGGA T^CCGGA
MscI 6373 TGGCCA TGG^CCA
NspV 327 TTCGAA TT^CGAA
PaeR7I 4379 CTCGAG C^TCGAG
PshBI 4027 ATTAAT AT^TAAT
SbfI 3669 CCTGCAGG CCTGCA^GG
SfuI 327 TTCGAA TT^CGAA
SphI 3691 GCATGC GCATG^C
Sse8387I 3669 CCTGCAGG CCTGCA^GG
XbaI 5521 TCTAGA T^CTAGA
XhoI 4379 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 820 CGCG CG^CG
6000 CGCG
AcyI 2739 GGCGTC G[GA]^CG[CT]C
2781 GACGTC
AflIII 4215 ACGTGT A^C[GA][CT]GT
4960 ACATGT
AlwI 6130 GATCC GGATC(4/5)
677 GGATC
Asp700I 1331 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3219 GAATGGCTTC
AvaI 4223 CTCGGG C^[CT]CG[GA]G
4379 CTCGAG
BbrPI 1264 CACGTG CAC^GTG
3889 CACGTG
BclI 4930 TGATCA T^GATCA
5390 TGATCA
BcnI 396 CCCGG CC^[GC]GG
2194 CCGGG
BglI 2708 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4593 GCCTCCTGGGC
BlpI 2683 GCTTAGC GC^T[ACGT]AGC
5411 GCTCAGC
Bpu1102I 2683 GCTTAGC GC^T[ACGT]AGC
5411 GCTCAGC
BsaAI 1264 CACGTG [CT]AC^GT[GA]
3889 CACGTG
BsaHI 2739 GGCGTC G[GA]^CG[CT]C
2781 GACGTC
BsmI 907 GAATGC GAATGC(1/-1)
3077 GAATGC
BsoBI 4223 CTCGGG C^[CT]CG[GA]G
4379 CTCGAG
BspHI 1138 TCATGA T^CATGA
2720 TCATGA
BsrDI 1682 GCAATG GCAATG(2/0)
1797 GCAATG
BstUI 820 CGCG CG^CG
6000 CGCG
CelII 2683 GCTTAGC GC^T[ACGT]AGC
5411 GCTCAGC
DraIII 3476 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
3478 CACGGTGTG
DrdI 2555 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4682 GACTCCCATGTC
FauI 5181 CCCGC CCCGC(4/6)
821 GCGGG
FbaI 4930 TGATCA T^GATCA
5390 TGATCA
HaeII 2680 GGCGCT [GA]GCGC^[CT]
6457 GGCGCT
Hin1I 2739 GGCGTC G[GA]^CG[CT]C
2781 GACGTC
HpaI 4956 GTTAAC GTT^AAC
5478 GTTAAC
MvnI 820 CGCG CG^CG
6000 CGCG
NciI 396 CCCGG CC^[GC]GG
2194 CCGGG
NcoI 974 CCATGG C^CATGG
3556 CCATGG
PflMI 3389 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4687 CCATGTCTTGG
PmaCI 1264 CACGTG CAC^GTG
3889 CACGTG
PmlI 1264 CACGTG CAC^GTG
3889 CACGTG
RcaI 1138 TCATGA T^CATGA
2720 TCATGA
SapI 2204 GAAGAGC GCTCTTC(1/4)
2435 GCTCTTC
ScaI 3866 AGTACT AGT^ACT
5105 AGTACT
SspI 567 AATATT AAT^ATT
5494 AATATT
StuI 279 AGGCCT AGG^CCT
6100 AGGCCT
Van91I 3389 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4687 CCATGTCTTGG
XmnI 1331 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3219 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AhdI
AluI AlwNI AocI ApoI AspEI
AspHI AvaII BanII BbsI BbvCI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII