################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AclI AgeI Aor51HI ApaI AscI Asp718I AspI AvrII BamHI BbeI BlnI BsiEI BsiWI BsmBI BspDI BsrBI BssHII Bst1107I BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI KasI KpnI KspI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SexAI SfiI SfoI SgrAI SmaI SnaBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 2781 GACGTC GACGT^C AccIII 1990 TCCGGA T^CCGGA AflII 1070 CTTAAG C^TTAAG Alw44I 6225 GTGCAC G^TGCAC ApaLI 6225 GTGCAC G^TGCAC AseI 4027 ATTAAT AT^TAAT AsnI 4027 ATTAAT AT^TAAT AviII 3144 TGCGCA TGC^GCA BalI 6373 TGGCCA TGG^CCA BanI 3803 GGTGCC G^G[CT][GA]CC BcgI 2590 GCAGAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 969 GTATCC GTATCC(6/5) BfrI 1070 CTTAAG C^TTAAG BsaBI 2597 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1990 TCCGGA [AT]^CCGG[AT] BseAI 1990 TCCGGA T^CCGGA BspEI 1990 TCCGGA T^CCGGA BspLU11I 4960 ACATGT A^CATGT BssSI 2733 CTCGTG CACGAG(-5/-1) BstBI 327 TTCGAA TT^CGAA BstEII 1654 GGTCACC G^GT[ACGT]ACC BstPI 1654 GGTCACC G^GT[ACGT]ACC BstXI 5051 CCAAATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoO65I 1654 GGTCACC G^GT[ACGT]ACC FspI 3144 TGCGCA TGC^GCA MamI 2597 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 6373 TGGCCA TGG^CCA MroI 1990 TCCGGA T^CCGGA MscI 6373 TGGCCA TGG^CCA NspV 327 TTCGAA TT^CGAA PaeR7I 4379 CTCGAG C^TCGAG PshBI 4027 ATTAAT AT^TAAT SbfI 3669 CCTGCAGG CCTGCA^GG SfuI 327 TTCGAA TT^CGAA SphI 3691 GCATGC GCATG^C Sse8387I 3669 CCTGCAGG CCTGCA^GG XbaI 5521 TCTAGA T^CTAGA XhoI 4379 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccII 820 CGCG CG^CG 6000 CGCG AcyI 2739 GGCGTC G[GA]^CG[CT]C 2781 GACGTC AflIII 4215 ACGTGT A^C[GA][CT]GT 4960 ACATGT AlwI 6130 GATCC GGATC(4/5) 677 GGATC Asp700I 1331 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3219 GAATGGCTTC AvaI 4223 CTCGGG C^[CT]CG[GA]G 4379 CTCGAG BbrPI 1264 CACGTG CAC^GTG 3889 CACGTG BclI 4930 TGATCA T^GATCA 5390 TGATCA BcnI 396 CCCGG CC^[GC]GG 2194 CCGGG BglI 2708 GCCCGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4593 GCCTCCTGGGC BlpI 2683 GCTTAGC GC^T[ACGT]AGC 5411 GCTCAGC Bpu1102I 2683 GCTTAGC GC^T[ACGT]AGC 5411 GCTCAGC BsaAI 1264 CACGTG [CT]AC^GT[GA] 3889 CACGTG BsaHI 2739 GGCGTC G[GA]^CG[CT]C 2781 GACGTC BsmI 907 GAATGC GAATGC(1/-1) 3077 GAATGC BsoBI 4223 CTCGGG C^[CT]CG[GA]G 4379 CTCGAG BspHI 1138 TCATGA T^CATGA 2720 TCATGA BsrDI 1682 GCAATG GCAATG(2/0) 1797 GCAATG BstUI 820 CGCG CG^CG 6000 CGCG CelII 2683 GCTTAGC GC^T[ACGT]AGC 5411 GCTCAGC DraIII 3476 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 3478 CACGGTGTG DrdI 2555 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4682 GACTCCCATGTC FauI 5181 CCCGC CCCGC(4/6) 821 GCGGG FbaI 4930 TGATCA T^GATCA 5390 TGATCA HaeII 2680 GGCGCT [GA]GCGC^[CT] 6457 GGCGCT Hin1I 2739 GGCGTC G[GA]^CG[CT]C 2781 GACGTC HpaI 4956 GTTAAC GTT^AAC 5478 GTTAAC MvnI 820 CGCG CG^CG 6000 CGCG NciI 396 CCCGG CC^[GC]GG 2194 CCGGG NcoI 974 CCATGG C^CATGG 3556 CCATGG PflMI 3389 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4687 CCATGTCTTGG PmaCI 1264 CACGTG CAC^GTG 3889 CACGTG PmlI 1264 CACGTG CAC^GTG 3889 CACGTG RcaI 1138 TCATGA T^CATGA 2720 TCATGA SapI 2204 GAAGAGC GCTCTTC(1/4) 2435 GCTCTTC ScaI 3866 AGTACT AGT^ACT 5105 AGTACT SspI 567 AATATT AAT^ATT 5494 AATATT StuI 279 AGGCCT AGG^CCT 6100 AGGCCT Van91I 3389 CCAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4687 CCATGTCTTGG XmnI 1331 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3219 GAATGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AciI AcsI AfaI AhdI AluI AlwNI AocI ApoI AspEI AspHI AvaII BanII BbsI BbvCI BbvI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII