##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BalI BbrPI BbvCI BcgI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KspI
MamI MfeI MluI MluNI MscI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SspBI SspI StuI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1352 GTCTAC GT^[AC][GT]AC
AccII 1332 CGCG CG^CG
AccIII 262 TCCGGA T^CCGGA
AocI 1250 CCTGAGG CC^T[ACGT]AGG
AspI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 475 GGATCC G^GATCC
BbsI 1520 GTCTTC GAAGAC(2/6)
BpuAI 1520 GTCTTC GAAGAC(2/6)
BseAI 262 TCCGGA T^CCGGA
BspEI 262 TCCGGA T^CCGGA
BssHII 1331 GCGCGC G^CGCGC
BssSI 173 CACGAG CACGAG(-5/-1)
BstAPI 1322 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 562 GGTGACC G^GT[ACGT]ACC
BstPI 562 GGTGACC G^GT[ACGT]ACC
BstUI 1332 CGCG CG^CG
BstYI 475 GGATCC [GA]^GATC[CT]
Bsu36I 1250 CCTGAGG CC^T[ACGT]AGG
DrdI 1212 GACCCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1250 CCTGAGG CC^T[ACGT]AGG
EcoO65I 562 GGTGACC G^GT[ACGT]ACC
HgaI 1434 GCGTC GACGC(5/10)
HincII 1513 GTTGAC GT[CT]^[GA]AC
HindII 1513 GTTGAC GT[CT]^[GA]AC
MaeII 583 ACGT A^CGT
MflI 475 GGATCC [GA]^GATC[CT]
MroI 262 TCCGGA T^CCGGA
MseI 1586 TTAA T^TAA
MvnI 1332 CGCG CG^CG
NcoI 783 CCATGG C^CATGG
PflFI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1074 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 689 CCTGCAGG CCTGCA^GG
SexAI 574 ACCTGGT A^CC[AT]GGT
SmlI 1648 CTCAAG C^T[CT][GA]AG
SpeI 1716 ACTAGT A^CTAGT
Sse8387I 689 CCTGCAGG CCTGCA^GG
TaqI 880 TCGA T^CGA
TfiI 469 GATTC G^A[AT]TC
Tru9I 1586 TTAA T^TAA
Tsp509I 1588 AATT ^AATT
Tth111I 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 880 TCGA T^CGA
Van91I 1074 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 475 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 399 GGTACC G^GTACC
1314 GGTACC
Alw44I 170 GTGCAC G^TGCAC
633 GTGCAC
AlwI 476 GATCC GGATC(4/5)
475 GGATC
ApaI 125 GGGCCC GGGCC^C
754 GGGCCC
ApaLI 170 GTGCAC G^TGCAC
633 GTGCAC
Asp718I 399 GGTACC G^GTACC
1314 GGTACC
AvaI 129 CCCGAG C^[CT]CG[GA]G
436 CTCGGG
BciVI 1375 GGATAC GTATCC(6/5)
1556 GGATAC
BglI 127 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
271 GCCAGTGGGGC
BsaWI 262 TCCGGA [AT]^CCGG[AT]
629 TCCGGT
BseRI 1541 CTCCTC GAGGAG(10/8)
1097 GAGGAG
BsiEI 456 CGGCCG CG[GA][CT]^CG
709 CGGCCG
BsmAI 432 GAGAC GTCTC(1/5)
1619 GAGAC
BsoBI 129 CCCGAG C^[CT]CG[GA]G
436 CTCGGG
BsrDI 778 CATTGC GCAATG(2/0)
621 GCAATG
BsrFI 771 ACCGGC [GA]^CCGG[CT]
1424 ACCGGC
Cfr10I 771 ACCGGC [GA]^CCGG[CT]
1424 ACCGGC
DsaI 567 CCGTGG C^C[GA][CT]GG
783 CCATGG
EagI 456 CGGCCG C^GGCCG
709 CGGCCG
EarI 508 GAAGAG CTCTTC(1/4)
1465 GAAGAG
EclXI 456 CGGCCG C^GGCCG
709 CGGCCG
Eco52I 456 CGGCCG C^GGCCG
709 CGGCCG
EcoT14I 69 CCAAGG C^C[AT][AT]GG
783 CCATGG
FauI 836 CCCGC CCCGC(4/6)
1042 GCGGG
KpnI 399 GGTACC GGTAC^C
1314 GGTACC
Ksp632I 508 GAAGAG CTCTTC(1/4)
1465 GAAGAG
NheI 355 GCTAGC G^CTAGC
982 GCTAGC
PstI 101 CTGCAG CTGCA^G
690 CTGCAG
SphI 1297 GCATGC GCATG^C
1366 GCATGC
StyI 69 CCAAGG C^C[AT][AT]GG
783 CCATGG
Tsp45I 563 GTGAC ^GT[GC]AC
1346 GTCAC
XcmI 1074 CCAGCTGGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1535 CCACCCCTCCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbeI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
TseI TspRI