################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MluNI MscI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SfiI SfuI SgrAI SmaI SnaBI SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 1352 GTCTAC GT^[AC][GT]AC AccII 1332 CGCG CG^CG AccIII 262 TCCGGA T^CCGGA AocI 1250 CCTGAGG CC^T[ACGT]AGG AspI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 475 GGATCC G^GATCC BbsI 1520 GTCTTC GAAGAC(2/6) BpuAI 1520 GTCTTC GAAGAC(2/6) BseAI 262 TCCGGA T^CCGGA BspEI 262 TCCGGA T^CCGGA BssHII 1331 GCGCGC G^CGCGC BssSI 173 CACGAG CACGAG(-5/-1) BstAPI 1322 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 562 GGTGACC G^GT[ACGT]ACC BstPI 562 GGTGACC G^GT[ACGT]ACC BstUI 1332 CGCG CG^CG BstYI 475 GGATCC [GA]^GATC[CT] Bsu36I 1250 CCTGAGG CC^T[ACGT]AGG DrdI 1212 GACCCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1250 CCTGAGG CC^T[ACGT]AGG EcoO65I 562 GGTGACC G^GT[ACGT]ACC HgaI 1434 GCGTC GACGC(5/10) HincII 1513 GTTGAC GT[CT]^[GA]AC HindII 1513 GTTGAC GT[CT]^[GA]AC MaeII 583 ACGT A^CGT MflI 475 GGATCC [GA]^GATC[CT] MroI 262 TCCGGA T^CCGGA MseI 1586 TTAA T^TAA MvnI 1332 CGCG CG^CG NcoI 783 CCATGG C^CATGG PflFI 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1074 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SbfI 689 CCTGCAGG CCTGCA^GG SexAI 574 ACCTGGT A^CC[AT]GGT SmlI 1648 CTCAAG C^T[CT][GA]AG SpeI 1716 ACTAGT A^CTAGT Sse8387I 689 CCTGCAGG CCTGCA^GG TaqI 880 TCGA T^CGA TfiI 469 GATTC G^A[AT]TC Tru9I 1586 TTAA T^TAA Tsp509I 1588 AATT ^AATT Tth111I 541 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 880 TCGA T^CGA Van91I 1074 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 475 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 399 GGTACC G^GTACC 1314 GGTACC Alw44I 170 GTGCAC G^TGCAC 633 GTGCAC AlwI 476 GATCC GGATC(4/5) 475 GGATC ApaI 125 GGGCCC GGGCC^C 754 GGGCCC ApaLI 170 GTGCAC G^TGCAC 633 GTGCAC Asp718I 399 GGTACC G^GTACC 1314 GGTACC AvaI 129 CCCGAG C^[CT]CG[GA]G 436 CTCGGG BciVI 1375 GGATAC GTATCC(6/5) 1556 GGATAC BglI 127 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 271 GCCAGTGGGGC BsaWI 262 TCCGGA [AT]^CCGG[AT] 629 TCCGGT BseRI 1541 CTCCTC GAGGAG(10/8) 1097 GAGGAG BsiEI 456 CGGCCG CG[GA][CT]^CG 709 CGGCCG BsmAI 432 GAGAC GTCTC(1/5) 1619 GAGAC BsoBI 129 CCCGAG C^[CT]CG[GA]G 436 CTCGGG BsrDI 778 CATTGC GCAATG(2/0) 621 GCAATG BsrFI 771 ACCGGC [GA]^CCGG[CT] 1424 ACCGGC Cfr10I 771 ACCGGC [GA]^CCGG[CT] 1424 ACCGGC DsaI 567 CCGTGG C^C[GA][CT]GG 783 CCATGG EagI 456 CGGCCG C^GGCCG 709 CGGCCG EarI 508 GAAGAG CTCTTC(1/4) 1465 GAAGAG EclXI 456 CGGCCG C^GGCCG 709 CGGCCG Eco52I 456 CGGCCG C^GGCCG 709 CGGCCG EcoT14I 69 CCAAGG C^C[AT][AT]GG 783 CCATGG FauI 836 CCCGC CCCGC(4/6) 1042 GCGGG KpnI 399 GGTACC GGTAC^C 1314 GGTACC Ksp632I 508 GAAGAG CTCTTC(1/4) 1465 GAAGAG NheI 355 GCTAGC G^CTAGC 982 GCTAGC PstI 101 CTGCAG CTGCA^G 690 CTGCAG SphI 1297 GCATGC GCATG^C 1366 GCATGC StyI 69 CCAAGG C^C[AT][AT]GG 783 CCATGG Tsp45I 563 GTGAC ^GT[GC]AC 1346 GTCAC XcmI 1074 CCAGCTGGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1535 CCACCCCTCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcyI AfaI AluI AlwNI AspHI AvaII BanI BanII BbeI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI TseI TspRI