##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AatII AflII AhdI AscI AspEI
AvrII BbrPI BclI BfrI BlnI
BsaAI BsaBI BsiWI Bsp1407I BspDI
BsrGI ClaI DrdI Eam1105I EcoRV
FbaI FseI HpaI MamI MfeI
MluI MunI NdeI NruI PacI
PmaCI PmeI PmlI PvuI SbfI
ScaI SfiI SnaBI SpeI Sse8387I
SspBI SspI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 4065 TCCGGA T^CCGGA
AclI 528 AACGTT AA^CGTT
AcsI 5192 GAATTC [GA]^AATT[CT]
AflIII 1497 ACATGT A^C[GA][CT]GT
AgeI 264 ACCGGT A^CCGGT
Alw44I 3236 GTGCAC G^TGCAC
ApaLI 3236 GTGCAC G^TGCAC
ApoI 5192 GAATTC [GA]^AATT[CT]
AseI 953 ATTAAT AT^TAAT
AsnI 953 ATTAAT AT^TAAT
BamHI 3398 GGATCC G^GATCC
BbeI 1878 GGCGCC GGCGC^C
BcgI 5302 GCAACTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 88 GGATAC GTATCC(6/5)
BglII 1342 AGATCT A^GATCT
BseAI 4065 TCCGGA T^CCGGA
BsiEI 32 CGGCCG CG[GA][CT]^CG
BspEI 4065 TCCGGA T^CCGGA
BspLU11I 1497 ACATGT A^CATGT
BssHII 5689 GCGCGC G^CGCGC
BstBI 5603 TTCGAA TT^CGAA
CpoI 4067 CGGACCG CG^G[AT]CCG
DraI 6676 TTTAAA TTT^AAA
DraIII 3449 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 32 CGGCCG C^GGCCG
EclXI 32 CGGCCG C^GGCCG
Eco52I 32 CGGCCG C^GGCCG
EcoRI 5192 GAATTC G^AATTC
EcoT22I 3227 ATGCAT ATGCA^T
HindIII 3911 AAGCTT A^AGCTT
KasI 1878 GGCGCC G^GCGCC
KspI 6975 CCGCGG CCGC^GG
MroI 4065 TCCGGA T^CCGGA
NarI 1878 GGCGCC GG^CGCC
NotI 31 GCGGCCGC GC^GGCCGC
NsiI 3227 ATGCAT ATGCA^T
NspV 5603 TTCGAA TT^CGAA
PinAI 264 ACCGGT A^CCGGT
PshBI 953 ATTAAT AT^TAAT
Psp1406I 528 AACGTT AA^CGTT
RsrII 4067 CGGACCG CG^G[AT]CCG
SacII 6975 CCGCGG CCGC^GG
SfoI 1878 GGCGCC GGC^GCC
SfuI 5603 TTCGAA TT^CGAA
SgrAI 3470 CGCCGGCG C[GA]^CCGG[CT]G
SphI 237 GCATGC GCATG^C
SwaI 6675 ATTTAAAT ATTT^AAAT
XbaI 1336 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 3124 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3817 GAAGCTCTTC
AviII 3420 TGCGCA TGC^GCA
3903 TGCGCA
BbvCI 4583 GCTGAGG CCTCAGC(-5/-2)
7034 GCTGAGG
BsaWI 264 ACCGGT [AT]^CCGG[AT]
4065 TCCGGA
BspHI 129 TCATGA T^CATGA
1761 TCATGA
Bst1107I 1358 GTATAC GTA^TAC
4797 GTATAC
BstEII 3437 GGTTACC G^GT[ACGT]ACC
6548 GGTTACC
BstPI 3437 GGTTACC G^GT[ACGT]ACC
6548 GGTTACC
BstZ17I 1358 GTATAC GTA^TAC
4797 GTATAC
EcoO65I 3437 GGTTACC G^GT[ACGT]ACC
6548 GGTTACC
FspI 3420 TGCGCA TGC^GCA
3903 TGCGCA
NaeI 1108 GCCGGC GCC^GGC
3471 GCCGGC
NgoMIV 1108 GCCGGC G^CCGGC
3471 GCCGGC
NheI 1409 GCTAGC G^CTAGC
5795 GCTAGC
PaeR7I 690 CTCGAG C^TCGAG
4949 CTCGAG
PshAI 681 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
7088 GACTGGAGTC
RcaI 129 TCATGA T^CATGA
1761 TCATGA
SalI 5310 GTCGAC G^TCGAC
5999 GTCGAC
SmaI 3331 CCCGGG CCC^GGG
5415 CCCGGG
XhoI 690 CTCGAG C^TCGAG
4949 CTCGAG
XmaCI 3331 CCCGGG C^CCGGG
5415 CCCGGG
XmaI 3331 CCCGGG C^CCGGG
5415 CCCGGG
XmnI 3124 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3817 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI AocI
Aor51HI ApaI Asp718I AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII