################################## # NO CUT ENZYMES ( 42 enzymes) # ################################## AatII AflII AhdI AscI AspEI AvrII BbrPI BclI BfrI BlnI BsaAI BsaBI BsiWI Bsp1407I BspDI BsrGI ClaI DrdI Eam1105I EcoRV FbaI FseI HpaI MamI MfeI MluI MunI NdeI NruI PacI PmaCI PmeI PmlI PvuI SbfI ScaI SfiI SnaBI SpeI Sse8387I SspBI SspI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 4065 TCCGGA T^CCGGA AclI 528 AACGTT AA^CGTT AcsI 5192 GAATTC [GA]^AATT[CT] AflIII 1497 ACATGT A^C[GA][CT]GT AgeI 264 ACCGGT A^CCGGT Alw44I 3236 GTGCAC G^TGCAC ApaLI 3236 GTGCAC G^TGCAC ApoI 5192 GAATTC [GA]^AATT[CT] AseI 953 ATTAAT AT^TAAT AsnI 953 ATTAAT AT^TAAT BamHI 3398 GGATCC G^GATCC BbeI 1878 GGCGCC GGCGC^C BcgI 5302 GCAACTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 88 GGATAC GTATCC(6/5) BglII 1342 AGATCT A^GATCT BseAI 4065 TCCGGA T^CCGGA BsiEI 32 CGGCCG CG[GA][CT]^CG BspEI 4065 TCCGGA T^CCGGA BspLU11I 1497 ACATGT A^CATGT BssHII 5689 GCGCGC G^CGCGC BstBI 5603 TTCGAA TT^CGAA CpoI 4067 CGGACCG CG^G[AT]CCG DraI 6676 TTTAAA TTT^AAA DraIII 3449 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 32 CGGCCG C^GGCCG EclXI 32 CGGCCG C^GGCCG Eco52I 32 CGGCCG C^GGCCG EcoRI 5192 GAATTC G^AATTC EcoT22I 3227 ATGCAT ATGCA^T HindIII 3911 AAGCTT A^AGCTT KasI 1878 GGCGCC G^GCGCC KspI 6975 CCGCGG CCGC^GG MroI 4065 TCCGGA T^CCGGA NarI 1878 GGCGCC GG^CGCC NotI 31 GCGGCCGC GC^GGCCGC NsiI 3227 ATGCAT ATGCA^T NspV 5603 TTCGAA TT^CGAA PinAI 264 ACCGGT A^CCGGT PshBI 953 ATTAAT AT^TAAT Psp1406I 528 AACGTT AA^CGTT RsrII 4067 CGGACCG CG^G[AT]CCG SacII 6975 CCGCGG CCGC^GG SfoI 1878 GGCGCC GGC^GCC SfuI 5603 TTCGAA TT^CGAA SgrAI 3470 CGCCGGCG C[GA]^CCGG[CT]G SphI 237 GCATGC GCATG^C SwaI 6675 ATTTAAAT ATTT^AAAT XbaI 1336 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Asp700I 3124 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3817 GAAGCTCTTC AviII 3420 TGCGCA TGC^GCA 3903 TGCGCA BbvCI 4583 GCTGAGG CCTCAGC(-5/-2) 7034 GCTGAGG BsaWI 264 ACCGGT [AT]^CCGG[AT] 4065 TCCGGA BspHI 129 TCATGA T^CATGA 1761 TCATGA Bst1107I 1358 GTATAC GTA^TAC 4797 GTATAC BstEII 3437 GGTTACC G^GT[ACGT]ACC 6548 GGTTACC BstPI 3437 GGTTACC G^GT[ACGT]ACC 6548 GGTTACC BstZ17I 1358 GTATAC GTA^TAC 4797 GTATAC EcoO65I 3437 GGTTACC G^GT[ACGT]ACC 6548 GGTTACC FspI 3420 TGCGCA TGC^GCA 3903 TGCGCA NaeI 1108 GCCGGC GCC^GGC 3471 GCCGGC NgoMIV 1108 GCCGGC G^CCGGC 3471 GCCGGC NheI 1409 GCTAGC G^CTAGC 5795 GCTAGC PaeR7I 690 CTCGAG C^TCGAG 4949 CTCGAG PshAI 681 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 7088 GACTGGAGTC RcaI 129 TCATGA T^CATGA 1761 TCATGA SalI 5310 GTCGAC G^TCGAC 5999 GTCGAC SmaI 3331 CCCGGG CCC^GGG 5415 CCCGGG XhoI 690 CTCGAG C^TCGAG 4949 CTCGAG XmaCI 3331 CCCGGG C^CCGGG 5415 CCCGGG XmaI 3331 CCCGGG C^CCGGG 5415 CCCGGG XmnI 3124 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3817 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 145 enzymes) # ######################################### Acc65I AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI Asp718I AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII