##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AclI AgeI AlwNI
AscI AseI AsnI Asp718I AviII
BbeI BclI BglII BlpI Bpu1102I
BsiWI Bsp1407I BspDI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MluI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I RsrII
SacII SbfI SfiI SfoI SfuI
SnaBI SphI Sse8387I SspBI SspI
SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 4318 TCCGGA T^CCGGA
AcyI 664 GACGCC G[GA]^CG[CT]C
Alw44I 947 GTGCAC G^TGCAC
ApaLI 947 GTGCAC G^TGCAC
AspI 5460 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3644 CCTAGG C^CTAGG
BanI 742 GGCACC G^G[CT][GA]CC
BbrPI 776 CACGTG CAC^GTG
BcgI 1297 GCAAGAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 5438 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3644 CCTAGG C^CTAGG
BsaAI 776 CACGTG [CT]AC^GT[GA]
BsaBI 2009 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 664 GACGCC G[GA]^CG[CT]C
BsaWI 4318 TCCGGA [AT]^CCGG[AT]
BseAI 4318 TCCGGA T^CCGGA
BsiEI 2573 CGATCG CG[GA][CT]^CG
BsmI 3339 GAATGC GAATGC(1/-1)
BspEI 4318 TCCGGA T^CCGGA
BspLU11I 3145 ACATGT A^CATGT
Hin1I 664 GACGCC G[GA]^CG[CT]C
MamI 2009 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 578 CAATTG C^AATTG
MroI 4318 TCCGGA T^CCGGA
MunI 578 CAATTG C^AATTG
NaeI 4172 GCCGGC GCC^GGC
NgoMIV 4172 GCCGGC G^CCGGC
PaeR7I 3195 CTCGAG C^TCGAG
PflFI 5460 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4276 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 776 CACGTG CAC^GTG
PmlI 776 CACGTG CAC^GTG
PshAI 5122 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2573 CGATCG CGAT^CG
SalI 5457 GTCGAC G^TCGAC
ScaI 5275 AGTACT AGT^ACT
SexAI 817 ACCTGGT A^CC[AT]GGT
SgrAI 1598 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3727 CCCGGG CCC^GGG
SpeI 4622 ACTAGT A^CTAGT
Tth111I 5460 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4276 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4839 TCTAGA T^CTAGA
XhoI 3195 CTCGAG C^TCGAG
XmaCI 3727 CCCGGG C^CCGGG
XmaI 3727 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1350 CGCG CG^CG
1839 CGCG
AflII 71 CTTAAG C^TTAAG
5561 CTTAAG
AflIII 775 ACACGT A^C[GA][CT]GT
3145 ACATGT
AhdI 3503 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4628 GACTCAATGTC
ApaI 269 GGGCCC GGGCC^C
4949 GGGCCC
AspEI 3503 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4628 GACTCAATGTC
BalI 1139 TGGCCA TGG^CCA
3378 TGGCCA
BbsI 3703 GAAGAC GAAGAC(2/6)
3277 GTCTTC
BfrI 71 CTTAAG C^TTAAG
5561 CTTAAG
BpuAI 3703 GAAGAC GAAGAC(2/6)
3277 GTCTTC
BsmBI 255 GAGACG CGTCTC(1/5)
4959 GAGACG
BspMI 1153 ACCTGC ACCTGC(4/8)
1865 ACCTGC
BsrBI 5722 CCGCTC CCGCTC(-3/-3)
765 GAGCGG
BsrFI 1599 ACCGGC [GA]^CCGG[CT]
4172 GCCGGC
BstEII 2859 GGTGACC G^GT[ACGT]ACC
3250 GGTGACC
BstPI 2859 GGTGACC G^GT[ACGT]ACC
3250 GGTGACC
BstUI 1350 CGCG CG^CG
1839 CGCG
BstXI 2447 CCATGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4696 CCAGAAGGGTGG
Cfr10I 1599 ACCGGC [GA]^CCGG[CT]
4172 GCCGGC
Eam1105I 3503 GACTGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4628 GACTCAATGTC
EcoO65I 2859 GGTGACC G^GT[ACGT]ACC
3250 GGTGACC
HgaI 664 GACGC GACGC(5/10)
1241 GACGC
HincII 3984 GTCAAC GT[CT]^[GA]AC
5457 GTCGAC
HindII 3984 GTCAAC GT[CT]^[GA]AC
5457 GTCGAC
MluNI 1139 TGGCCA TGG^CCA
3378 TGGCCA
MscI 1139 TGGCCA TGG^CCA
3378 TGGCCA
MvnI 1350 CGCG CG^CG
1839 CGCG
NspI 3145 ACATGT [GA]CATG^[CT]
4893 GCATGT
XcmI 173 CCATCTGAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4186 CCAGGCTGCCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI Aor51HI ApoI Asp700I
AspHI AvaI AvaII BamHI BanII
BbvCI BbvI BciVI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI