##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AcsI AflII AgeI ApoI AscI
AseI AsnI Asp700I BamHI BbeI
BciVI BfrI BsaBI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DrdI EcoO65I EcoRI EcoRV EcoT22I
FseI KasI KspI MamI MluI
NarI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
PvuI SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SpeI
Sse8387I SspBI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 3988 GGTACC G^GTACC
AflIII 2752 ACGTGT A^C[GA][CT]GT
Aor51HI 4447 AGCGCT AGC^GCT
Asp718I 3988 GGTACC G^GTACC
AspI 3027 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1622 TGGCCA TGG^CCA
BanI 3988 GGTACC G^G[CT][GA]CC
BbrPI 3220 CACGTG CAC^GTG
BclI 818 TGATCA T^GATCA
BglII 5535 AGATCT A^GATCT
BsaI 3463 GAGACC GGTCTC(1/5)
BsrFI 5547 GCCGGC [GA]^CCGG[CT]
Cfr10I 5547 GCCGGC [GA]^CCGG[CT]
CpoI 1218 CGGTCCG CG^G[AT]CCG
Eco47III 4447 AGCGCT AGC^GCT
FbaI 818 TGATCA T^GATCA
KpnI 3988 GGTACC GGTAC^C
MluNI 1622 TGGCCA TGG^CCA
MscI 1622 TGGCCA TGG^CCA
NaeI 5547 GCCGGC GCC^GGC
NcoI 1768 CCATGG C^CATGG
NgoMIV 5547 GCCGGC G^CCGGC
NheI 3515 GCTAGC G^CTAGC
NspI 4807 GCATGC [GA]CATG^[CT]
PflFI 3027 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3220 CACGTG CAC^GTG
PmlI 3220 CACGTG CAC^GTG
RsrII 1218 CGGTCCG CG^G[AT]CCG
SnaBI 793 TACGTA TAC^GTA
SphI 4807 GCATGC GCATG^C
SspI 2400 AATATT AAT^ATT
Tth111I 3027 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3063 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2865 GACGTC GACGT^C
4739 GACGTC
AclI 32 AACGTT AA^CGTT
5452 AACGTT
AcyI 2865 GACGTC G[GA]^CG[CT]C
4739 GACGTC
Alw44I 1494 GTGCAC G^TGCAC
3199 GTGCAC
ApaI 918 GGGCCC GGGCC^C
4018 GGGCCC
ApaLI 1494 GTGCAC G^TGCAC
3199 GTGCAC
AvrII 3407 CCTAGG C^CTAGG
5858 CCTAGG
BlnI 3407 CCTAGG C^CTAGG
5858 CCTAGG
BsaHI 2865 GACGTC G[GA]^CG[CT]C
4739 GACGTC
BsiEI 166 CGGCCG CG[GA][CT]^CG
4601 CGGCCG
BsmI 3773 GAATGC GAATGC(1/-1)
2267 GCATTC
BspHI 2924 TCATGA T^CATGA
4858 TCATGA
BsrDI 2975 CATTGC GCAATG(2/0)
3090 GCAATG
DraI 4085 TTTAAA TTT^AAA
6089 TTTAAA
EagI 166 CGGCCG C^GGCCG
4601 CGGCCG
EclXI 166 CGGCCG C^GGCCG
4601 CGGCCG
Eco52I 166 CGGCCG C^GGCCG
4601 CGGCCG
Hin1I 2865 GACGTC G[GA]^CG[CT]C
4739 GACGTC
HindIII 92 AAGCTT A^AGCTT
4697 AAGCTT
HpaI 2495 GTTAAC GTT^AAC
2909 GTTAAC
MfeI 1038 CAATTG C^AATTG
3585 CAATTG
MunI 1038 CAATTG C^AATTG
3585 CAATTG
PshAI 6 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6095 GACTGTTGTC
Psp1406I 32 AACGTT AA^CGTT
5452 AACGTT
RcaI 2924 TCATGA T^CATGA
4858 TCATGA
SapI 4196 GAAGAGC GCTCTTC(1/4)
1264 GCTCTTC
SfiI 910 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3953 GGCCCTCCAGGCC
XcmI 337 CCAAGGACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5669 CCAGCAAAGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AccIII AciI AfaI
AhdI AluI AlwI AlwNI AocI
AspEI AspHI AvaI AvaII AviII
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoI
XhoII XmaCI XmaI