################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AcsI AflII AgeI ApoI AscI AseI AsnI Asp700I BamHI BbeI BciVI BfrI BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI DrdI EcoO65I EcoRI EcoRV EcoT22I FseI KasI KspI MamI MluI NarI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshBI PvuI SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SpeI Sse8387I SspBI SwaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 3988 GGTACC G^GTACC AflIII 2752 ACGTGT A^C[GA][CT]GT Aor51HI 4447 AGCGCT AGC^GCT Asp718I 3988 GGTACC G^GTACC AspI 3027 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1622 TGGCCA TGG^CCA BanI 3988 GGTACC G^G[CT][GA]CC BbrPI 3220 CACGTG CAC^GTG BclI 818 TGATCA T^GATCA BglII 5535 AGATCT A^GATCT BsaI 3463 GAGACC GGTCTC(1/5) BsrFI 5547 GCCGGC [GA]^CCGG[CT] Cfr10I 5547 GCCGGC [GA]^CCGG[CT] CpoI 1218 CGGTCCG CG^G[AT]CCG Eco47III 4447 AGCGCT AGC^GCT FbaI 818 TGATCA T^GATCA KpnI 3988 GGTACC GGTAC^C MluNI 1622 TGGCCA TGG^CCA MscI 1622 TGGCCA TGG^CCA NaeI 5547 GCCGGC GCC^GGC NcoI 1768 CCATGG C^CATGG NgoMIV 5547 GCCGGC G^CCGGC NheI 3515 GCTAGC G^CTAGC NspI 4807 GCATGC [GA]CATG^[CT] PflFI 3027 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3220 CACGTG CAC^GTG PmlI 3220 CACGTG CAC^GTG RsrII 1218 CGGTCCG CG^G[AT]CCG SnaBI 793 TACGTA TAC^GTA SphI 4807 GCATGC GCATG^C SspI 2400 AATATT AAT^ATT Tth111I 3027 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3063 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AatII 2865 GACGTC GACGT^C 4739 GACGTC AclI 32 AACGTT AA^CGTT 5452 AACGTT AcyI 2865 GACGTC G[GA]^CG[CT]C 4739 GACGTC Alw44I 1494 GTGCAC G^TGCAC 3199 GTGCAC ApaI 918 GGGCCC GGGCC^C 4018 GGGCCC ApaLI 1494 GTGCAC G^TGCAC 3199 GTGCAC AvrII 3407 CCTAGG C^CTAGG 5858 CCTAGG BlnI 3407 CCTAGG C^CTAGG 5858 CCTAGG BsaHI 2865 GACGTC G[GA]^CG[CT]C 4739 GACGTC BsiEI 166 CGGCCG CG[GA][CT]^CG 4601 CGGCCG BsmI 3773 GAATGC GAATGC(1/-1) 2267 GCATTC BspHI 2924 TCATGA T^CATGA 4858 TCATGA BsrDI 2975 CATTGC GCAATG(2/0) 3090 GCAATG DraI 4085 TTTAAA TTT^AAA 6089 TTTAAA EagI 166 CGGCCG C^GGCCG 4601 CGGCCG EclXI 166 CGGCCG C^GGCCG 4601 CGGCCG Eco52I 166 CGGCCG C^GGCCG 4601 CGGCCG Hin1I 2865 GACGTC G[GA]^CG[CT]C 4739 GACGTC HindIII 92 AAGCTT A^AGCTT 4697 AAGCTT HpaI 2495 GTTAAC GTT^AAC 2909 GTTAAC MfeI 1038 CAATTG C^AATTG 3585 CAATTG MunI 1038 CAATTG C^AATTG 3585 CAATTG PshAI 6 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 6095 GACTGTTGTC Psp1406I 32 AACGTT AA^CGTT 5452 AACGTT RcaI 2924 TCATGA T^CATGA 4858 TCATGA SapI 4196 GAAGAGC GCTCTTC(1/4) 1264 GCTCTTC SfiI 910 GGCCTCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3953 GGCCCTCCAGGCC XcmI 337 CCAAGGACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5669 CCAGCAAAGACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccI AccII AccIII AciI AfaI AhdI AluI AlwI AlwNI AocI AspEI AspHI AvaI AvaII AviII BanII BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI XhoII XmaCI XmaI