##################################
# NO CUT ENZYMES (   50 enzymes) #
##################################

AatII       AclI        AflII       AscI        AseI        
AsnI        AviII       AvrII       BbeI        BcgI        
BciVI       BfrI        BlnI        BsaBI       BsiWI       
Bsp1407I    BspDI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        EcoRV       
FspI        KasI        MamI        NarI        NotI        
NruI        NspV        PacI        PmeI        PshAI       
PshBI       Psp1406I    RsrII       SalI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       3104  GGTACC               G^GTACC
AccI          936  GTCTAC               GT^[AC][GT]AC
AccIII       3576  TCCGGA               T^CCGGA
AgeI          220  ACCGGT               A^CCGGT
AhdI         4716  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2303  AGCGCT               AGC^GCT
Asp718I      3104  GGTACC               G^GTACC
AspEI        4716  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          996  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BseAI        3576  TCCGGA               T^CCGGA
BspEI        3576  TCCGGA               T^CCGGA
DraI         6492  TTTAAA               TTT^AAA
DrdI         1522  GACAGAGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3593  CGGCCG               C^GGCCG
Eam1105I     4716  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3593  CGGCCG               C^GGCCG
Eco47III     2303  AGCGCT               AGC^GCT
Eco52I       3593  CGGCCG               C^GGCCG
FseI         4597  GGCCGGCC             GGCCGG^CC
HincII       4261  GTTAAC               GT[CT]^[GA]AC
HindII       4261  GTTAAC               GT[CT]^[GA]AC
HpaI         4261  GTTAAC               GTT^AAC
KpnI         3104  GGTACC               GGTAC^C
MfeI         3088  CAATTG               C^AATTG
MluI         2714  ACGCGT               A^CGCGT
MroI         3576  TCCGGA               T^CCGGA
MunI         3088  CAATTG               C^AATTG
NcoI         1931  CCATGG               C^CATGG
NheI         1641  GCTAGC               G^CTAGC
PaeR7I       5323  CTCGAG               C^TCGAG
PflFI         996  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         220  ACCGGT               A^CCGGT
PvuI         2750  CGATCG               CGAT^CG
ScaI         2723  AGTACT               AGT^ACT
SmaI         3384  CCCGGG               CCC^GGG
Tth111I       996  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4823  TCTAGA               T^CTAGA
XhoI         5323  CTCGAG               C^TCGAG
XmaCI        3384  CCCGGG               C^CCGGG
XmaI         3384  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI         2395  GACGCC               G[GA]^CG[CT]C
             2731  GACGCC              
BanI         1303  GGCACC               G^G[CT][GA]CC
             3104  GGTACC              
BbrPI        2982  CACGTG               CAC^GTG
             3037  CACGTG              
BclI         3605  TGATCA               T^GATCA
             5026  TGATCA              
BglII        2993  AGATCT               A^GATCT
             6457  AGATCT              
BsaAI        2982  CACGTG               [CT]AC^GT[GA]
             3037  CACGTG              
BsaHI        2395  GACGCC               G[GA]^CG[CT]C
             2731  GACGCC              
BsiEI        2750  CGATCG               CG[GA][CT]^CG
             3593  CGGCCG              
BsmBI         652  GAGACG               CGTCTC(1/5)
             3619  GAGACG              
BspHI        2645  TCATGA               T^CATGA
             5973  TCATGA              
BspLU11I     2028  ACATGT               A^CATGT
             2458  ACATGT              
EcoT22I      5040  ATGCAT               ATGCA^T
             6175  ATGCAT              
FbaI         3605  TGATCA               T^GATCA
             5026  TGATCA              
Hin1I        2395  GACGCC               G[GA]^CG[CT]C
             2731  GACGCC              
HindIII      1506  AAGCTT               A^AGCTT
             6272  AAGCTT              
KspI         2400  CCGCGG               CCGC^GG
             6933  CCGCGG              
NaeI         1300  GCCGGC               GCC^GGC
             4598  GCCGGC              
NdeI         2795  CATATG               CA^TATG
             5214  CATATG              
NgoMIV       1300  GCCGGC               G^CCGGC
             4598  GCCGGC              
NsiI         5040  ATGCAT               ATGCA^T
             6175  ATGCAT              
PmaCI        2982  CACGTG               CAC^GTG
             3037  CACGTG              
PmlI         2982  CACGTG               CAC^GTG
             3037  CACGTG              
RcaI         2645  TCATGA               T^CATGA
             5973  TCATGA              
SacII        2400  CCGCGG               CCGC^GG
             6933  CCGCGG              
SmlI         5323  CTCGAG               C^T[CT][GA]AG
             6894  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  143 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaI        ApaLI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BamHI       BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SphI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII       XmnI