##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI AviII AvrII BbeI BcgI
BciVI BfrI BlnI BsaBI BsiWI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EcoRV
FspI KasI MamI NarI NotI
NruI NspV PacI PmeI PshAI
PshBI Psp1406I RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3104 GGTACC G^GTACC
AccI 936 GTCTAC GT^[AC][GT]AC
AccIII 3576 TCCGGA T^CCGGA
AgeI 220 ACCGGT A^CCGGT
AhdI 4716 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2303 AGCGCT AGC^GCT
Asp718I 3104 GGTACC G^GTACC
AspEI 4716 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 996 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BseAI 3576 TCCGGA T^CCGGA
BspEI 3576 TCCGGA T^CCGGA
DraI 6492 TTTAAA TTT^AAA
DrdI 1522 GACAGAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3593 CGGCCG C^GGCCG
Eam1105I 4716 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3593 CGGCCG C^GGCCG
Eco47III 2303 AGCGCT AGC^GCT
Eco52I 3593 CGGCCG C^GGCCG
FseI 4597 GGCCGGCC GGCCGG^CC
HincII 4261 GTTAAC GT[CT]^[GA]AC
HindII 4261 GTTAAC GT[CT]^[GA]AC
HpaI 4261 GTTAAC GTT^AAC
KpnI 3104 GGTACC GGTAC^C
MfeI 3088 CAATTG C^AATTG
MluI 2714 ACGCGT A^CGCGT
MroI 3576 TCCGGA T^CCGGA
MunI 3088 CAATTG C^AATTG
NcoI 1931 CCATGG C^CATGG
NheI 1641 GCTAGC G^CTAGC
PaeR7I 5323 CTCGAG C^TCGAG
PflFI 996 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 220 ACCGGT A^CCGGT
PvuI 2750 CGATCG CGAT^CG
ScaI 2723 AGTACT AGT^ACT
SmaI 3384 CCCGGG CCC^GGG
Tth111I 996 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4823 TCTAGA T^CTAGA
XhoI 5323 CTCGAG C^TCGAG
XmaCI 3384 CCCGGG C^CCGGG
XmaI 3384 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 2395 GACGCC G[GA]^CG[CT]C
2731 GACGCC
BanI 1303 GGCACC G^G[CT][GA]CC
3104 GGTACC
BbrPI 2982 CACGTG CAC^GTG
3037 CACGTG
BclI 3605 TGATCA T^GATCA
5026 TGATCA
BglII 2993 AGATCT A^GATCT
6457 AGATCT
BsaAI 2982 CACGTG [CT]AC^GT[GA]
3037 CACGTG
BsaHI 2395 GACGCC G[GA]^CG[CT]C
2731 GACGCC
BsiEI 2750 CGATCG CG[GA][CT]^CG
3593 CGGCCG
BsmBI 652 GAGACG CGTCTC(1/5)
3619 GAGACG
BspHI 2645 TCATGA T^CATGA
5973 TCATGA
BspLU11I 2028 ACATGT A^CATGT
2458 ACATGT
EcoT22I 5040 ATGCAT ATGCA^T
6175 ATGCAT
FbaI 3605 TGATCA T^GATCA
5026 TGATCA
Hin1I 2395 GACGCC G[GA]^CG[CT]C
2731 GACGCC
HindIII 1506 AAGCTT A^AGCTT
6272 AAGCTT
KspI 2400 CCGCGG CCGC^GG
6933 CCGCGG
NaeI 1300 GCCGGC GCC^GGC
4598 GCCGGC
NdeI 2795 CATATG CA^TATG
5214 CATATG
NgoMIV 1300 GCCGGC G^CCGGC
4598 GCCGGC
NsiI 5040 ATGCAT ATGCA^T
6175 ATGCAT
PmaCI 2982 CACGTG CAC^GTG
3037 CACGTG
PmlI 2982 CACGTG CAC^GTG
3037 CACGTG
RcaI 2645 TCATGA T^CATGA
5973 TCATGA
SacII 2400 CCGCGG CCGC^GG
6933 CCGCGG
SmlI 5323 CTCGAG C^T[CT][GA]AG
6894 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI