################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AatII AclI AflII AscI AseI AsnI AviII AvrII BbeI BcgI BciVI BfrI BlnI BsaBI BsiWI Bsp1407I BspDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EcoRV FspI KasI MamI NarI NotI NruI NspV PacI PmeI PshAI PshBI Psp1406I RsrII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 3104 GGTACC G^GTACC AccI 936 GTCTAC GT^[AC][GT]AC AccIII 3576 TCCGGA T^CCGGA AgeI 220 ACCGGT A^CCGGT AhdI 4716 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 2303 AGCGCT AGC^GCT Asp718I 3104 GGTACC G^GTACC AspEI 4716 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 996 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BseAI 3576 TCCGGA T^CCGGA BspEI 3576 TCCGGA T^CCGGA DraI 6492 TTTAAA TTT^AAA DrdI 1522 GACAGAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3593 CGGCCG C^GGCCG Eam1105I 4716 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3593 CGGCCG C^GGCCG Eco47III 2303 AGCGCT AGC^GCT Eco52I 3593 CGGCCG C^GGCCG FseI 4597 GGCCGGCC GGCCGG^CC HincII 4261 GTTAAC GT[CT]^[GA]AC HindII 4261 GTTAAC GT[CT]^[GA]AC HpaI 4261 GTTAAC GTT^AAC KpnI 3104 GGTACC GGTAC^C MfeI 3088 CAATTG C^AATTG MluI 2714 ACGCGT A^CGCGT MroI 3576 TCCGGA T^CCGGA MunI 3088 CAATTG C^AATTG NcoI 1931 CCATGG C^CATGG NheI 1641 GCTAGC G^CTAGC PaeR7I 5323 CTCGAG C^TCGAG PflFI 996 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 220 ACCGGT A^CCGGT PvuI 2750 CGATCG CGAT^CG ScaI 2723 AGTACT AGT^ACT SmaI 3384 CCCGGG CCC^GGG Tth111I 996 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4823 TCTAGA T^CTAGA XhoI 5323 CTCGAG C^TCGAG XmaCI 3384 CCCGGG C^CCGGG XmaI 3384 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 2395 GACGCC G[GA]^CG[CT]C 2731 GACGCC BanI 1303 GGCACC G^G[CT][GA]CC 3104 GGTACC BbrPI 2982 CACGTG CAC^GTG 3037 CACGTG BclI 3605 TGATCA T^GATCA 5026 TGATCA BglII 2993 AGATCT A^GATCT 6457 AGATCT BsaAI 2982 CACGTG [CT]AC^GT[GA] 3037 CACGTG BsaHI 2395 GACGCC G[GA]^CG[CT]C 2731 GACGCC BsiEI 2750 CGATCG CG[GA][CT]^CG 3593 CGGCCG BsmBI 652 GAGACG CGTCTC(1/5) 3619 GAGACG BspHI 2645 TCATGA T^CATGA 5973 TCATGA BspLU11I 2028 ACATGT A^CATGT 2458 ACATGT EcoT22I 5040 ATGCAT ATGCA^T 6175 ATGCAT FbaI 3605 TGATCA T^GATCA 5026 TGATCA Hin1I 2395 GACGCC G[GA]^CG[CT]C 2731 GACGCC HindIII 1506 AAGCTT A^AGCTT 6272 AAGCTT KspI 2400 CCGCGG CCGC^GG 6933 CCGCGG NaeI 1300 GCCGGC GCC^GGC 4598 GCCGGC NdeI 2795 CATATG CA^TATG 5214 CATATG NgoMIV 1300 GCCGGC G^CCGGC 4598 GCCGGC NsiI 5040 ATGCAT ATGCA^T 6175 ATGCAT PmaCI 2982 CACGTG CAC^GTG 3037 CACGTG PmlI 2982 CACGTG CAC^GTG 3037 CACGTG RcaI 2645 TCATGA T^CATGA 5973 TCATGA SacII 2400 CCGCGG CCGC^GG 6933 CCGCGG SmlI 5323 CTCGAG C^T[CT][GA]AG 6894 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI