##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AcyI Aor51HI AscI
AvaI AviII BbeI BbrPI BcgI
BciVI BclI BsaHI BsiEI BsiWI
BsoBI BspLU11I BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoT22I FbaI FseI FspI
Hin1I KasI KspI MluI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 2846 GTAGAC GT^[AC][GT]AC
AccII 1273 CGCG CG^CG
AccIII 1290 TCCGGA T^CCGGA
AflIII 2014 ACACGT A^C[GA][CT]GT
AgeI 2462 ACCGGT A^CCGGT
AhdI 5888 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 15 GTGCAC G^TGCAC
ApaLI 15 GTGCAC G^TGCAC
AseI 4941 ATTAAT AT^TAAT
AsnI 4941 ATTAAT AT^TAAT
AspEI 5888 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3520 GGATCC G^GATCC
BbvCI 3898 CCTCAGC CCTCAGC(-5/-2)
BglI 41 GCCGCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 910 AGATCT A^GATCT
BsaBI 4038 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1290 TCCGGA T^CCGGA
BspDI 935 ATCGAT AT^CGAT
BspEI 1290 TCCGGA T^CCGGA
BspHI 3864 TCATGA T^CATGA
BsrFI 2462 ACCGGT [GA]^CCGG[CT]
BstAPI 6191 GCACCGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1273 CGCG CG^CG
Cfr10I 2462 ACCGGT [GA]^CCGG[CT]
ClaI 935 ATCGAT AT^CGAT
Eam1105I 5888 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1815 GATATC GAT^ATC
HaeII 2553 AGCGCC [GA]GCGC^[CT]
HpaI 487 GTTAAC GTT^AAC
MamI 4038 GATACCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1931 CAATTG C^AATTG
MroI 1290 TCCGGA T^CCGGA
MunI 1931 CAATTG C^AATTG
MvnI 1273 CGCG CG^CG
PinAI 2462 ACCGGT A^CCGGT
PshBI 4941 ATTAAT AT^TAAT
RcaI 3864 TCATGA T^CATGA
SexAI 5352 ACCTGGT A^CC[AT]GGT
SnaBI 3601 TACGTA TAC^GTA
SphI 1022 GCATGC GCATG^C
SspI 836 AATATT AAT^ATT
XbaI 999 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 541 GGTACC G^GTACC
3150 GGTACC
AflII 4395 CTTAAG C^TTAAG
5367 CTTAAG
Asp700I 187 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4803 GAAAATCTTC
Asp718I 541 GGTACC G^GTACC
3150 GGTACC
AvrII 2759 CCTAGG C^CTAGG
3653 CCTAGG
BfrI 4395 CTTAAG C^TTAAG
5367 CTTAAG
BlnI 2759 CCTAGG C^CTAGG
3653 CCTAGG
BsaAI 18 CACGTA [CT]AC^GT[GA]
3601 TACGTA
BsaI 4175 GAGACC GGTCTC(1/5)
5054 GGTCTC
BsaWI 1290 TCCGGA [AT]^CCGG[AT]
2462 ACCGGT
BseRI 1874 CTCCTC GAGGAG(10/8)
4423 GAGGAG
BspMI 3776 ACCTGC ACCTGC(4/8)
4147 ACCTGC
HgaI 2245 GACGC GACGC(5/10)
5507 GACGC
HincII 487 GTTAAC GT[CT]^[GA]AC
2386 GTCAAC
HindII 487 GTTAAC GT[CT]^[GA]AC
2386 GTCAAC
HindIII 856 AAGCTT A^AGCTT
1951 AAGCTT
KpnI 541 GGTACC GGTAC^C
3150 GGTACC
NheI 873 GCTAGC G^CTAGC
5484 GCTAGC
PpuMI 2559 AGGTCCT [GA]G^G[AT]CC[CT]
5071 GGGTCCC
PshAI 2257 GACAACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5090 GACTCAGGTC
SacI 4220 GAGCTC GAGCT^C
4326 GAGCTC
SapI 1695 GAAGAGC GCTCTTC(1/4)
1779 GCTCTTC
StuI 5103 AGGCCT AGG^CCT
5432 AGGCCT
XmnI 187 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4803 GAAAATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AspI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII