################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccIII AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspI BamHI BbrPI BclI BfrI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I Cfr10I CpoI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI HpaI KspI MluI MroI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 5940 GACGTC GACGT^C AccI 5360 GTCTAC GT^[AC][GT]AC AccII 1924 CGCG CG^CG AviII 2596 TGCGCA TGC^GCA BalI 4140 TGGCCA TGG^CCA BbsI 1282 GTCTTC GAAGAC(2/6) BbvCI 1996 GCTGAGG CCTCAGC(-5/-2) BcgI 4452 CGAGCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 3553 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1282 GTCTTC GAAGAC(2/6) BsaAI 3775 CACGTA [CT]AC^GT[GA] BsaBI 4436 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4223 ACCGGA [AT]^CCGG[AT] BsmBI 1057 GAGACG CGTCTC(1/5) BspDI 2839 ATCGAT AT^CGAT BstUI 1924 CGCG CG^CG BstXI 1407 CCAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 2839 ATCGAT AT^CGAT DsaI 579 CCATGG C^C[GA][CT]GG EcoT22I 5343 ATGCAT ATGCA^T FspI 2596 TGCGCA TGC^GCA HindIII 4927 AAGCTT A^AGCTT MamI 4436 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2962 CAATTG C^AATTG MluNI 4140 TGGCCA TGG^CCA MscI 4140 TGGCCA TGG^CCA MunI 2962 CAATTG C^AATTG MvnI 1924 CGCG CG^CG NcoI 579 CCATGG C^CATGG NsiI 5343 ATGCAT ATGCA^T PshAI 2384 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1125 GAAGAGC GCTCTTC(1/4) ScaI 4832 AGTACT AGT^ACT SmaI 1621 CCCGGG CCC^GGG SphI 5341 GCATGC GCATG^C XbaI 109 TCTAGA T^CTAGA XmaCI 1621 CCCGGG C^CCGGG XmaI 1621 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 1698 GGTACC G^GTACC 2776 GGTACC AclI 1259 AACGTT AA^CGTT 2588 AACGTT AhdI 1012 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4503 GACACGAGGTC Asp700I 2815 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3287 GAAGAGATTC Asp718I 1698 GGTACC G^GTACC 2776 GGTACC AspEI 1012 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4503 GACACGAGGTC AvrII 190 CCTAGG C^CTAGG 3394 CCTAGG BciVI 3336 GTATCC GTATCC(6/5) 3952 GTATCC BlnI 190 CCTAGG C^CTAGG 3394 CCTAGG BseRI 5168 CTCCTC GAGGAG(10/8) 172 GAGGAG BsrBI 2915 CCGCTC CCGCTC(-3/-3) 3252 CCGCTC BsrDI 616 CATTGC GCAATG(2/0) 280 GCAATG BstAPI 1657 GCATCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5460 GCAGGAGCTGC BstEII 1142 GGTTACC G^GT[ACGT]ACC 3370 GGTGACC BstPI 1142 GGTTACC G^GT[ACGT]ACC 3370 GGTGACC DrdI 3411 GACAACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3965 GACCTCCTGGTC EaeI 576 CGGCCA [CT]^GGCC[GA] 4140 TGGCCA Eam1105I 1012 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4503 GACACGAGGTC EcoNI 3211 CCTTCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3933 CCTTCTCCAGG EcoO65I 1142 GGTTACC G^GT[ACGT]ACC 3370 GGTGACC FauI 1395 CCCGC CCCGC(4/6) 3251 CCCGC HgaI 1390 GCGTC GACGC(5/10) 4988 GCGTC KpnI 1698 GGTACC GGTAC^C 2776 GGTACC NdeI 87 CATATG CA^TATG 587 CATATG NheI 3997 GCTAGC G^CTAGC 5242 GCTAGC Psp1406I 1259 AACGTT AA^CGTT 2588 AACGTT PvuII 1798 CAGCTG CAG^CTG 4213 CAGCTG SacI 1640 GAGCTC GAGCT^C 2026 GAGCTC StuI 2431 AGGCCT AGG^CCT 4537 AGGCCT XmnI 2815 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3287 GAAGAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BssSI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII