##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccIII AflII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspI BamHI BbrPI BclI BfrI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspEI BspHI BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I EcoRV FbaI FseI
HpaI KspI MluI MroI NaeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 5940 GACGTC GACGT^C
AccI 5360 GTCTAC GT^[AC][GT]AC
AccII 1924 CGCG CG^CG
AviII 2596 TGCGCA TGC^GCA
BalI 4140 TGGCCA TGG^CCA
BbsI 1282 GTCTTC GAAGAC(2/6)
BbvCI 1996 GCTGAGG CCTCAGC(-5/-2)
BcgI 4452 CGAGCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3553 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1282 GTCTTC GAAGAC(2/6)
BsaAI 3775 CACGTA [CT]AC^GT[GA]
BsaBI 4436 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4223 ACCGGA [AT]^CCGG[AT]
BsmBI 1057 GAGACG CGTCTC(1/5)
BspDI 2839 ATCGAT AT^CGAT
BstUI 1924 CGCG CG^CG
BstXI 1407 CCAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2839 ATCGAT AT^CGAT
DsaI 579 CCATGG C^C[GA][CT]GG
EcoT22I 5343 ATGCAT ATGCA^T
FspI 2596 TGCGCA TGC^GCA
HindIII 4927 AAGCTT A^AGCTT
MamI 4436 GATTCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2962 CAATTG C^AATTG
MluNI 4140 TGGCCA TGG^CCA
MscI 4140 TGGCCA TGG^CCA
MunI 2962 CAATTG C^AATTG
MvnI 1924 CGCG CG^CG
NcoI 579 CCATGG C^CATGG
NsiI 5343 ATGCAT ATGCA^T
PshAI 2384 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1125 GAAGAGC GCTCTTC(1/4)
ScaI 4832 AGTACT AGT^ACT
SmaI 1621 CCCGGG CCC^GGG
SphI 5341 GCATGC GCATG^C
XbaI 109 TCTAGA T^CTAGA
XmaCI 1621 CCCGGG C^CCGGG
XmaI 1621 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1698 GGTACC G^GTACC
2776 GGTACC
AclI 1259 AACGTT AA^CGTT
2588 AACGTT
AhdI 1012 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4503 GACACGAGGTC
Asp700I 2815 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3287 GAAGAGATTC
Asp718I 1698 GGTACC G^GTACC
2776 GGTACC
AspEI 1012 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4503 GACACGAGGTC
AvrII 190 CCTAGG C^CTAGG
3394 CCTAGG
BciVI 3336 GTATCC GTATCC(6/5)
3952 GTATCC
BlnI 190 CCTAGG C^CTAGG
3394 CCTAGG
BseRI 5168 CTCCTC GAGGAG(10/8)
172 GAGGAG
BsrBI 2915 CCGCTC CCGCTC(-3/-3)
3252 CCGCTC
BsrDI 616 CATTGC GCAATG(2/0)
280 GCAATG
BstAPI 1657 GCATCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5460 GCAGGAGCTGC
BstEII 1142 GGTTACC G^GT[ACGT]ACC
3370 GGTGACC
BstPI 1142 GGTTACC G^GT[ACGT]ACC
3370 GGTGACC
DrdI 3411 GACAACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3965 GACCTCCTGGTC
EaeI 576 CGGCCA [CT]^GGCC[GA]
4140 TGGCCA
Eam1105I 1012 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4503 GACACGAGGTC
EcoNI 3211 CCTTCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3933 CCTTCTCCAGG
EcoO65I 1142 GGTTACC G^GT[ACGT]ACC
3370 GGTGACC
FauI 1395 CCCGC CCCGC(4/6)
3251 CCCGC
HgaI 1390 GCGTC GACGC(5/10)
4988 GCGTC
KpnI 1698 GGTACC GGTAC^C
2776 GGTACC
NdeI 87 CATATG CA^TATG
587 CATATG
NheI 3997 GCTAGC G^CTAGC
5242 GCTAGC
Psp1406I 1259 AACGTT AA^CGTT
2588 AACGTT
PvuII 1798 CAGCTG CAG^CTG
4213 CAGCTG
SacI 1640 GAGCTC GAGCT^C
2026 GAGCTC
StuI 2431 AGGCCT AGG^CCT
4537 AGGCCT
XmnI 2815 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3287 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BglII
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrI BssKI BssSI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII