################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AgeI AscI AseI AsnI AspI AviII AvrII BbrPI BclI BlnI BlpI Bpu1102I BsaAI BsiWI BspDI BsrBI Bst1107I BstZ17I CelII ClaI CpoI DraIII EcoT22I FbaI FseI FspI HpaI KspI MfeI MluI MunI NdeI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI RsrII SacI SacII SbfI ScaI SgrAI SnaBI Sse8387I SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 1652 CGCG CG^CG AccIII 1743 TCCGGA T^CCGGA AflII 6289 CTTAAG C^TTAAG AflIII 5200 ACATGT A^C[GA][CT]GT ApaI 2411 GGGCCC GGGCC^C BbvCI 1170 GCTGAGG CCTCAGC(-5/-2) BfrI 6289 CTTAAG C^TTAAG BseAI 1743 TCCGGA T^CCGGA BsmBI 1499 CGTCTC CGTCTC(1/5) Bsp1407I 3907 TGTACA T^GTACA BspEI 1743 TCCGGA T^CCGGA BspHI 3109 TCATGA T^CATGA BspLU11I 5200 ACATGT A^CATGT BsrGI 3907 TGTACA T^GTACA BssHII 1651 GCGCGC G^CGCGC BstEII 817 GGTAACC G^GT[ACGT]ACC BstPI 817 GGTAACC G^GT[ACGT]ACC BstUI 1652 CGCG CG^CG DrdI 442 GACAACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1165 CGGCCG C^GGCCG EclXI 1165 CGGCCG C^GGCCG Eco52I 1165 CGGCCG C^GGCCG EcoO65I 817 GGTAACC G^GT[ACGT]ACC MroI 1743 TCCGGA T^CCGGA MvnI 1652 CGCG CG^CG NaeI 3203 GCCGGC GCC^GGC NcoI 802 CCATGG C^CATGG NgoMIV 3203 GCCGGC G^CCGGC NheI 4896 GCTAGC G^CTAGC PshAI 1524 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 1122 CGATCG CGAT^CG RcaI 3109 TCATGA T^CATGA SalI 2700 GTCGAC G^TCGAC SapI 61 GAAGAGC GCTCTTC(1/4) SexAI 3124 ACCAGGT A^CC[AT]GGT SfiI 1166 GGCCGCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 2353 CCCGGG CCC^GGG SpeI 5293 ACTAGT A^CTAGT SspBI 3907 TGTACA T^GTACA XmaCI 2353 CCCGGG C^CCGGG XmaI 2353 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 2700 GTCGAC GT^[AC][GT]AC 5537 GTCTAC AcyI 3777 GGCGCC G[GA]^CG[CT]C 4130 GGCGCC AhdI 1 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3356 GACTGTCCGTC Alw44I 630 GTGCAC G^TGCAC 3102 GTGCAC AocI 2151 CCTGAGG CC^T[ACGT]AGG 5736 CCTGAGG ApaLI 630 GTGCAC G^TGCAC 3102 GTGCAC AspEI 1 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3356 GACTGTCCGTC BamHI 1278 GGATCC G^GATCC 2505 GGATCC BbeI 3777 GGCGCC GGCGC^C 4130 GGCGCC BciVI 80 GGATAC GTATCC(6/5) 4771 GTATCC BglII 616 AGATCT A^GATCT 3283 AGATCT BsaBI 1747 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4587 GATTGTAATC BsaHI 3777 GGCGCC G[GA]^CG[CT]C 4130 GGCGCC BsaWI 1142 ACCGGA [AT]^CCGG[AT] 1743 TCCGGA BsrDI 535 GCAATG GCAATG(2/0) 4686 GCAATG BsrFI 3203 GCCGGC [GA]^CCGG[CT] 3979 ACCGGC Bsu36I 2151 CCTGAGG CC^T[ACGT]AGG 5736 CCTGAGG Cfr10I 3203 GCCGGC [GA]^CCGG[CT] 3979 ACCGGC DsaI 802 CCATGG C^C[GA][CT]GG 5087 CCACGG Eam1105I 1 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3356 GACTGTCCGTC Eco81I 2151 CCTGAGG CC^T[ACGT]AGG 5736 CCTGAGG EcoNI 994 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4182 CCTACCAAAGG EcoRI 558 GAATTC G^AATTC 2605 GAATTC Hin1I 3777 GGCGCC G[GA]^CG[CT]C 4130 GGCGCC HincII 2700 GTCGAC GT[CT]^[GA]AC 4375 GTCAAC HindII 2700 GTCGAC GT[CT]^[GA]AC 4375 GTCAAC KasI 3777 GGCGCC G^GCGCC 4130 GGCGCC MamI 1747 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4587 GATTGTAATC NarI 3777 GGCGCC GG^CGCC 4130 GGCGCC PvuII 2028 CAGCTG CAG^CTG 2823 CAGCTG SfoI 3777 GGCGCC GGC^GCC 4130 GGCGCC SmlI 6082 CTTGAG C^T[CT][GA]AG 6289 CTTAAG StuI 1427 AGGCCT AGG^CCT 5824 AGGCCT XcmI 571 CCAGTGAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4738 CCAGAGCCACCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### Acc65I AciI AclI AcsI AfaI AluI AlwI AlwNI Aor51HI ApoI Asp700I Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstAPI BstBI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco47III EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI NspV PflMI PleI PpuMI Psp1406I PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SphI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI