##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AatII       AgeI        AscI        AseI        AsnI        
AspI        AviII       AvrII       BbrPI       BclI        
BlnI        BlpI        Bpu1102I    BsaAI       BsiWI       
BspDI       BsrBI       Bst1107I    BstZ17I     CelII       
ClaI        CpoI        DraIII      EcoT22I     FbaI        
FseI        FspI        HpaI        KspI        MfeI        
MluI        MunI        NdeI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       RsrII       
SacI        SacII       SbfI        ScaI        SgrAI       
SnaBI       Sse8387I    SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII        1652  CGCG                 CG^CG
AccIII       1743  TCCGGA               T^CCGGA
AflII        6289  CTTAAG               C^TTAAG
AflIII       5200  ACATGT               A^C[GA][CT]GT
ApaI         2411  GGGCCC               GGGCC^C
BbvCI        1170  GCTGAGG              CCTCAGC(-5/-2)
BfrI         6289  CTTAAG               C^TTAAG
BseAI        1743  TCCGGA               T^CCGGA
BsmBI        1499  CGTCTC               CGTCTC(1/5)
Bsp1407I     3907  TGTACA               T^GTACA
BspEI        1743  TCCGGA               T^CCGGA
BspHI        3109  TCATGA               T^CATGA
BspLU11I     5200  ACATGT               A^CATGT
BsrGI        3907  TGTACA               T^GTACA
BssHII       1651  GCGCGC               G^CGCGC
BstEII        817  GGTAACC              G^GT[ACGT]ACC
BstPI         817  GGTAACC              G^GT[ACGT]ACC
BstUI        1652  CGCG                 CG^CG
DrdI          442  GACAACCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1165  CGGCCG               C^GGCCG
EclXI        1165  CGGCCG               C^GGCCG
Eco52I       1165  CGGCCG               C^GGCCG
EcoO65I       817  GGTAACC              G^GT[ACGT]ACC
MroI         1743  TCCGGA               T^CCGGA
MvnI         1652  CGCG                 CG^CG
NaeI         3203  GCCGGC               GCC^GGC
NcoI          802  CCATGG               C^CATGG
NgoMIV       3203  GCCGGC               G^CCGGC
NheI         4896  GCTAGC               G^CTAGC
PshAI        1524  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         1122  CGATCG               CGAT^CG
RcaI         3109  TCATGA               T^CATGA
SalI         2700  GTCGAC               G^TCGAC
SapI           61  GAAGAGC              GCTCTTC(1/4)
SexAI        3124  ACCAGGT              A^CC[AT]GGT
SfiI         1166  GGCCGCTGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2353  CCCGGG               CCC^GGG
SpeI         5293  ACTAGT               A^CTAGT
SspBI        3907  TGTACA               T^GTACA
XmaCI        2353  CCCGGG               C^CCGGG
XmaI         2353  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         2700  GTCGAC               GT^[AC][GT]AC
             5537  GTCTAC              
AcyI         3777  GGCGCC               G[GA]^CG[CT]C
             4130  GGCGCC              
AhdI            1  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3356  GACTGTCCGTC         
Alw44I        630  GTGCAC               G^TGCAC
             3102  GTGCAC              
AocI         2151  CCTGAGG              CC^T[ACGT]AGG
             5736  CCTGAGG             
ApaLI         630  GTGCAC               G^TGCAC
             3102  GTGCAC              
AspEI           1  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3356  GACTGTCCGTC         
BamHI        1278  GGATCC               G^GATCC
             2505  GGATCC              
BbeI         3777  GGCGCC               GGCGC^C
             4130  GGCGCC              
BciVI          80  GGATAC               GTATCC(6/5)
             4771  GTATCC              
BglII         616  AGATCT               A^GATCT
             3283  AGATCT              
BsaBI        1747  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4587  GATTGTAATC          
BsaHI        3777  GGCGCC               G[GA]^CG[CT]C
             4130  GGCGCC              
BsaWI        1142  ACCGGA               [AT]^CCGG[AT]
             1743  TCCGGA              
BsrDI         535  GCAATG               GCAATG(2/0)
             4686  GCAATG              
BsrFI        3203  GCCGGC               [GA]^CCGG[CT]
             3979  ACCGGC              
Bsu36I       2151  CCTGAGG              CC^T[ACGT]AGG
             5736  CCTGAGG             
Cfr10I       3203  GCCGGC               [GA]^CCGG[CT]
             3979  ACCGGC              
DsaI          802  CCATGG               C^C[GA][CT]GG
             5087  CCACGG              
Eam1105I        1  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3356  GACTGTCCGTC         
Eco81I       2151  CCTGAGG              CC^T[ACGT]AGG
             5736  CCTGAGG             
EcoNI         994  CCTGGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4182  CCTACCAAAGG         
EcoRI         558  GAATTC               G^AATTC
             2605  GAATTC              
Hin1I        3777  GGCGCC               G[GA]^CG[CT]C
             4130  GGCGCC              
HincII       2700  GTCGAC               GT[CT]^[GA]AC
             4375  GTCAAC              
HindII       2700  GTCGAC               GT[CT]^[GA]AC
             4375  GTCAAC              
KasI         3777  GGCGCC               G^GCGCC
             4130  GGCGCC              
MamI         1747  GATGCCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4587  GATTGTAATC          
NarI         3777  GGCGCC               GG^CGCC
             4130  GGCGCC              
PvuII        2028  CAGCTG               CAG^CTG
             2823  CAGCTG              
SfoI         3777  GGCGCC               GGC^GCC
             4130  GGCGCC              
SmlI         6082  CTTGAG               C^T[CT][GA]AG
             6289  CTTAAG              
StuI         1427  AGGCCT               AGG^CCT
             5824  AGGCCT              
XcmI          571  CCAGTGAGTGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4738  CCAGAGCCACCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

Acc65I      AciI        AclI        AcsI        AfaI        
AluI        AlwI        AlwNI       Aor51HI     ApoI        
Asp700I     Asp718I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcgI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BssSI       BstAPI      
BstBI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EaeI        EarI        Eco47III    
EcoO109I    EcoRII      EcoRV       EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        KpnI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
NspV        PflMI       PleI        PpuMI       Psp1406I    
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfuI        SphI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI