##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AgeI AscI AseI AsnI
AspI AviII AvrII BbrPI BclI
BlnI BlpI Bpu1102I BsaAI BsiWI
BspDI BsrBI Bst1107I BstZ17I CelII
ClaI CpoI DraIII EcoT22I FbaI
FseI FspI HpaI KspI MfeI
MluI MunI NdeI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI RsrII
SacI SacII SbfI ScaI SgrAI
SnaBI Sse8387I SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 1652 CGCG CG^CG
AccIII 1743 TCCGGA T^CCGGA
AflII 6289 CTTAAG C^TTAAG
AflIII 5200 ACATGT A^C[GA][CT]GT
ApaI 2411 GGGCCC GGGCC^C
BbvCI 1170 GCTGAGG CCTCAGC(-5/-2)
BfrI 6289 CTTAAG C^TTAAG
BseAI 1743 TCCGGA T^CCGGA
BsmBI 1499 CGTCTC CGTCTC(1/5)
Bsp1407I 3907 TGTACA T^GTACA
BspEI 1743 TCCGGA T^CCGGA
BspHI 3109 TCATGA T^CATGA
BspLU11I 5200 ACATGT A^CATGT
BsrGI 3907 TGTACA T^GTACA
BssHII 1651 GCGCGC G^CGCGC
BstEII 817 GGTAACC G^GT[ACGT]ACC
BstPI 817 GGTAACC G^GT[ACGT]ACC
BstUI 1652 CGCG CG^CG
DrdI 442 GACAACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1165 CGGCCG C^GGCCG
EclXI 1165 CGGCCG C^GGCCG
Eco52I 1165 CGGCCG C^GGCCG
EcoO65I 817 GGTAACC G^GT[ACGT]ACC
MroI 1743 TCCGGA T^CCGGA
MvnI 1652 CGCG CG^CG
NaeI 3203 GCCGGC GCC^GGC
NcoI 802 CCATGG C^CATGG
NgoMIV 3203 GCCGGC G^CCGGC
NheI 4896 GCTAGC G^CTAGC
PshAI 1524 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1122 CGATCG CGAT^CG
RcaI 3109 TCATGA T^CATGA
SalI 2700 GTCGAC G^TCGAC
SapI 61 GAAGAGC GCTCTTC(1/4)
SexAI 3124 ACCAGGT A^CC[AT]GGT
SfiI 1166 GGCCGCTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2353 CCCGGG CCC^GGG
SpeI 5293 ACTAGT A^CTAGT
SspBI 3907 TGTACA T^GTACA
XmaCI 2353 CCCGGG C^CCGGG
XmaI 2353 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2700 GTCGAC GT^[AC][GT]AC
5537 GTCTAC
AcyI 3777 GGCGCC G[GA]^CG[CT]C
4130 GGCGCC
AhdI 1 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3356 GACTGTCCGTC
Alw44I 630 GTGCAC G^TGCAC
3102 GTGCAC
AocI 2151 CCTGAGG CC^T[ACGT]AGG
5736 CCTGAGG
ApaLI 630 GTGCAC G^TGCAC
3102 GTGCAC
AspEI 1 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3356 GACTGTCCGTC
BamHI 1278 GGATCC G^GATCC
2505 GGATCC
BbeI 3777 GGCGCC GGCGC^C
4130 GGCGCC
BciVI 80 GGATAC GTATCC(6/5)
4771 GTATCC
BglII 616 AGATCT A^GATCT
3283 AGATCT
BsaBI 1747 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4587 GATTGTAATC
BsaHI 3777 GGCGCC G[GA]^CG[CT]C
4130 GGCGCC
BsaWI 1142 ACCGGA [AT]^CCGG[AT]
1743 TCCGGA
BsrDI 535 GCAATG GCAATG(2/0)
4686 GCAATG
BsrFI 3203 GCCGGC [GA]^CCGG[CT]
3979 ACCGGC
Bsu36I 2151 CCTGAGG CC^T[ACGT]AGG
5736 CCTGAGG
Cfr10I 3203 GCCGGC [GA]^CCGG[CT]
3979 ACCGGC
DsaI 802 CCATGG C^C[GA][CT]GG
5087 CCACGG
Eam1105I 1 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3356 GACTGTCCGTC
Eco81I 2151 CCTGAGG CC^T[ACGT]AGG
5736 CCTGAGG
EcoNI 994 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4182 CCTACCAAAGG
EcoRI 558 GAATTC G^AATTC
2605 GAATTC
Hin1I 3777 GGCGCC G[GA]^CG[CT]C
4130 GGCGCC
HincII 2700 GTCGAC GT[CT]^[GA]AC
4375 GTCAAC
HindII 2700 GTCGAC GT[CT]^[GA]AC
4375 GTCAAC
KasI 3777 GGCGCC G^GCGCC
4130 GGCGCC
MamI 1747 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4587 GATTGTAATC
NarI 3777 GGCGCC GG^CGCC
4130 GGCGCC
PvuII 2028 CAGCTG CAG^CTG
2823 CAGCTG
SfoI 3777 GGCGCC GGC^GCC
4130 GGCGCC
SmlI 6082 CTTGAG C^T[CT][GA]AG
6289 CTTAAG
StuI 1427 AGGCCT AGG^CCT
5824 AGGCCT
XcmI 571 CCAGTGAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4738 CCAGAGCCACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AciI AclI AcsI AfaI
AluI AlwI AlwNI Aor51HI ApoI
Asp700I Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstAPI
BstBI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco47III
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
NspV PflMI PleI PpuMI Psp1406I
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI