##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccI AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI AspI
AviII AvrII BbeI BbrPI BlnI
BsaBI BsiWI BspDI BsrBI BssHII
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DraIII Eco47III EcoO65I FseI
FspI HpaI KasI MamI MluI
NarI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfoI SnaBI Sse8387I SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 3015 GGTACC G^GTACC
AccIII 924 TCCGGA T^CCGGA
AcyI 6103 GACGCC G[GA]^CG[CT]C
AflII 744 CTTAAG C^TTAAG
AhdI 2039 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2827 CCTCAGG CC^T[ACGT]AGG
Asp700I 5593 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3015 GGTACC G^GTACC
AspEI 2039 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 5721 GCAAATGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1368 TGATCA T^GATCA
BfrI 744 CTTAAG C^TTAAG
BglII 5886 AGATCT A^GATCT
BlpI 4156 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4156 GCTGAGC GC^T[ACGT]AGC
BsaAI 458 CACGTA [CT]AC^GT[GA]
BsaHI 6103 GACGCC G[GA]^CG[CT]C
BsaWI 924 TCCGGA [AT]^CCGG[AT]
BseAI 924 TCCGGA T^CCGGA
BsiEI 18 CGGCCG CG[GA][CT]^CG
BsmBI 1111 GAGACG CGTCTC(1/5)
Bsp1407I 3963 TGTACA T^GTACA
BspEI 924 TCCGGA T^CCGGA
BsrGI 3963 TGTACA T^GTACA
BssSI 2846 CTCGTG CACGAG(-5/-1)
Bsu36I 2827 CCTCAGG CC^T[ACGT]AGG
CelII 4156 GCTGAGC GC^T[ACGT]AGC
EagI 18 CGGCCG C^GGCCG
Eam1105I 2039 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
Eco81I 2827 CCTCAGG CC^T[ACGT]AGG
EcoT22I 3614 ATGCAT ATGCA^T
FbaI 1368 TGATCA T^GATCA
HaeII 398 GGCGCT [GA]GCGC^[CT]
Hin1I 6103 GACGCC G[GA]^CG[CT]C
KpnI 3015 GGTACC GGTAC^C
KspI 59 CCGCGG CCGC^GG
MfeI 1316 CAATTG C^AATTG
MroI 924 TCCGGA T^CCGGA
MunI 1316 CAATTG C^AATTG
NaeI 5422 GCCGGC GCC^GGC
NgoMIV 5422 GCCGGC G^CCGGC
NheI 3543 GCTAGC G^CTAGC
NsiI 3614 ATGCAT ATGCA^T
PshAI 1068 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 59 CCGCGG CCGC^GG
SexAI 5408 ACCAGGT A^CC[AT]GGT
SfiI 56 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 394 CACCGGCG C[GA]^CCGG[CT]G
SmaI 5660 CCCGGG CCC^GGG
SpeI 2010 ACTAGT A^CTAGT
SphI 718 GCATGC GCATG^C
SspBI 3963 TGTACA T^GTACA
XmaCI 5660 CCCGGG C^CCGGG
XmaI 5660 CCCGGG C^CCGGG
XmnI 5593 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 60 CGCG CG^CG
5568 CGCG
AflIII 1698 ACATGT A^C[GA][CT]GT
2378 ACATGT
AseI 2410 ATTAAT AT^TAAT
2739 ATTAAT
AsnI 2410 ATTAAT AT^TAAT
2739 ATTAAT
BciVI 4398 GGATAC GTATCC(6/5)
4521 GGATAC
BglI 41 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
57 GCCCGCGGGGC
BsgI 2601 CTGCAC GTGCAG(16/14)
4035 GTGCAG
BsmI 792 GAATGC GAATGC(1/-1)
3539 GAATGC
BspLU11I 1698 ACATGT A^CATGT
2378 ACATGT
BsrFI 395 ACCGGC [GA]^CCGG[CT]
5422 GCCGGC
BstAPI 4037 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5392 GCAGACAGTGC
BstBI 476 TTCGAA TT^CGAA
6203 TTCGAA
BstUI 60 CGCG CG^CG
5568 CGCG
Cfr10I 395 ACCGGC [GA]^CCGG[CT]
5422 GCCGGC
DrdI 3592 GACCCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4776 GACAACCCTGTC
EcoNI 2823 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4578 CCTCTGTGAGG
MvnI 60 CGCG CG^CG
5568 CGCG
NdeI 1061 CATATG CA^TATG
3040 CATATG
NspV 476 TTCGAA TT^CGAA
6203 TTCGAA
PaeR7I 2642 CTCGAG C^TCGAG
3320 CTCGAG
PpuMI 4322 AGGACCT [GA]G^G[AT]CC[CT]
4616 AGGACCT
PshBI 2410 ATTAAT AT^TAAT
2739 ATTAAT
SfuI 476 TTCGAA TT^CGAA
6203 TTCGAA
StuI 4648 AGGCCT AGG^CCT
5448 AGGCCT
XcmI 1182 CCATCACACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2492 CCAAACCCCACTTGG
XhoI 2642 CTCGAG C^TCGAG
3320 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII