################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AatII AccI AclI AgeI Alw44I Aor51HI ApaI ApaLI AscI AspI AviII AvrII BbeI BbrPI BlnI BsaBI BsiWI BspDI BsrBI BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraIII Eco47III EcoO65I FseI FspI HpaI KasI MamI MluI NarI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI ScaI SfoI SnaBI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 3015 GGTACC G^GTACC AccIII 924 TCCGGA T^CCGGA AcyI 6103 GACGCC G[GA]^CG[CT]C AflII 744 CTTAAG C^TTAAG AhdI 2039 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2827 CCTCAGG CC^T[ACGT]AGG Asp700I 5593 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3015 GGTACC G^GTACC AspEI 2039 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcgI 5721 GCAAATGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1368 TGATCA T^GATCA BfrI 744 CTTAAG C^TTAAG BglII 5886 AGATCT A^GATCT BlpI 4156 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4156 GCTGAGC GC^T[ACGT]AGC BsaAI 458 CACGTA [CT]AC^GT[GA] BsaHI 6103 GACGCC G[GA]^CG[CT]C BsaWI 924 TCCGGA [AT]^CCGG[AT] BseAI 924 TCCGGA T^CCGGA BsiEI 18 CGGCCG CG[GA][CT]^CG BsmBI 1111 GAGACG CGTCTC(1/5) Bsp1407I 3963 TGTACA T^GTACA BspEI 924 TCCGGA T^CCGGA BsrGI 3963 TGTACA T^GTACA BssSI 2846 CTCGTG CACGAG(-5/-1) Bsu36I 2827 CCTCAGG CC^T[ACGT]AGG CelII 4156 GCTGAGC GC^T[ACGT]AGC EagI 18 CGGCCG C^GGCCG Eam1105I 2039 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 18 CGGCCG C^GGCCG Eco52I 18 CGGCCG C^GGCCG Eco81I 2827 CCTCAGG CC^T[ACGT]AGG EcoT22I 3614 ATGCAT ATGCA^T FbaI 1368 TGATCA T^GATCA HaeII 398 GGCGCT [GA]GCGC^[CT] Hin1I 6103 GACGCC G[GA]^CG[CT]C KpnI 3015 GGTACC GGTAC^C KspI 59 CCGCGG CCGC^GG MfeI 1316 CAATTG C^AATTG MroI 924 TCCGGA T^CCGGA MunI 1316 CAATTG C^AATTG NaeI 5422 GCCGGC GCC^GGC NgoMIV 5422 GCCGGC G^CCGGC NheI 3543 GCTAGC G^CTAGC NsiI 3614 ATGCAT ATGCA^T PshAI 1068 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 59 CCGCGG CCGC^GG SexAI 5408 ACCAGGT A^CC[AT]GGT SfiI 56 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 394 CACCGGCG C[GA]^CCGG[CT]G SmaI 5660 CCCGGG CCC^GGG SpeI 2010 ACTAGT A^CTAGT SphI 718 GCATGC GCATG^C SspBI 3963 TGTACA T^GTACA XmaCI 5660 CCCGGG C^CCGGG XmaI 5660 CCCGGG C^CCGGG XmnI 5593 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 60 CGCG CG^CG 5568 CGCG AflIII 1698 ACATGT A^C[GA][CT]GT 2378 ACATGT AseI 2410 ATTAAT AT^TAAT 2739 ATTAAT AsnI 2410 ATTAAT AT^TAAT 2739 ATTAAT BciVI 4398 GGATAC GTATCC(6/5) 4521 GGATAC BglI 41 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 57 GCCCGCGGGGC BsgI 2601 CTGCAC GTGCAG(16/14) 4035 GTGCAG BsmI 792 GAATGC GAATGC(1/-1) 3539 GAATGC BspLU11I 1698 ACATGT A^CATGT 2378 ACATGT BsrFI 395 ACCGGC [GA]^CCGG[CT] 5422 GCCGGC BstAPI 4037 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5392 GCAGACAGTGC BstBI 476 TTCGAA TT^CGAA 6203 TTCGAA BstUI 60 CGCG CG^CG 5568 CGCG Cfr10I 395 ACCGGC [GA]^CCGG[CT] 5422 GCCGGC DrdI 3592 GACCCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4776 GACAACCCTGTC EcoNI 2823 CCTTCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4578 CCTCTGTGAGG MvnI 60 CGCG CG^CG 5568 CGCG NdeI 1061 CATATG CA^TATG 3040 CATATG NspV 476 TTCGAA TT^CGAA 6203 TTCGAA PaeR7I 2642 CTCGAG C^TCGAG 3320 CTCGAG PpuMI 4322 AGGACCT [GA]G^G[AT]CC[CT] 4616 AGGACCT PshBI 2410 ATTAAT AT^TAAT 2739 ATTAAT SfuI 476 TTCGAA TT^CGAA 6203 TTCGAA StuI 4648 AGGCCT AGG^CCT 5448 AGGCCT XcmI 1182 CCATCACACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2492 CCAAACCCCACTTGG XhoI 2642 CTCGAG C^TCGAG 3320 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII