##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AccIII AclI Aor51HI AscI BbeI
BseAI BsiWI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV FseI HaeII HindIII KasI
KspI MluI MroI NarI NheI
NotI NspV PacI PflMI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SmaI SpeI SphI Sse8387I SwaI
Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 5865 GACGTC GACGT^C
Acc65I 3359 GGTACC G^GTACC
AccI 2173 GTAGAC GT^[AC][GT]AC
AflII 5138 CTTAAG C^TTAAG
AhdI 6525 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2312 GTGCAC G^TGCAC
ApaI 2424 GGGCCC GGGCC^C
ApaLI 2312 GTGCAC G^TGCAC
AseI 6697 ATTAAT AT^TAAT
AsnI 6697 ATTAAT AT^TAAT
Asp700I 6187 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3359 GGTACC G^GTACC
AspEI 6525 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1260 TGCGCA TGC^GCA
AvrII 3015 CCTAGG C^CTAGG
BalI 190 TGGCCA TGG^CCA
BbrPI 2889 CACGTG CAC^GTG
BbvCI 463 CCTCAGC CCTCAGC(-5/-2)
BciVI 4340 GTATCC GTATCC(6/5)
BfrI 5138 CTTAAG C^TTAAG
BlnI 3015 CCTAGG C^CTAGG
BlpI 225 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 225 GCTCAGC GC^T[ACGT]AGC
BsaBI 4748 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 722 CGACCG CG[GA][CT]^CG
BspDI 2844 ATCGAT AT^CGAT
BspHI 5061 TCATGA T^CATGA
BsrBI 36 GAGCGG CCGCTC(-3/-3)
BsrDI 3225 GCAATG GCAATG(2/0)
BssSI 6119 CACGAG CACGAG(-5/-1)
BstAPI 5877 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 225 GCTCAGC GC^T[ACGT]AGC
ClaI 2844 ATCGAT AT^CGAT
Eam1105I 6525 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1260 TGCGCA TGC^GCA
HpaI 4151 GTTAAC GTT^AAC
KpnI 3359 GGTACC GGTAC^C
MamI 4748 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1336 CAATTG C^AATTG
MluNI 190 TGGCCA TGG^CCA
MscI 190 TGGCCA TGG^CCA
MunI 1336 CAATTG C^AATTG
NaeI 1587 GCCGGC GCC^GGC
NdeI 6703 CATATG CA^TATG
NgoMIV 1587 GCCGGC G^CCGGC
NruI 3691 TCGCGA TCG^CGA
PaeR7I 3310 CTCGAG C^TCGAG
PmaCI 2889 CACGTG CAC^GTG
PmlI 2889 CACGTG CAC^GTG
PshBI 6697 ATTAAT AT^TAAT
RcaI 5061 TCATGA T^CATGA
SexAI 2141 ACCAGGT A^CC[AT]GGT
SgrAI 5011 CACCGGTG C[GA]^CCGG[CT]G
SspI 4220 AATATT AAT^ATT
XcmI 3749 CCAGCAGGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3310 CTCGAG C^TCGAG
XmnI 6187 GAAGTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 1590 GGCGTC G[GA]^CG[CT]C
5865 GACGTC
AgeI 2085 ACCGGT A^CCGGT
5012 ACCGGT
AocI 1638 CCTGAGG CC^T[ACGT]AGG
5127 CCTCAGG
BcgI 2067 CGAGCGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3659 CGAACAGTTTGC
BglI 2426 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3011 GCCTCCTAGGC
BglII 1160 AGATCT A^GATCT
2606 AGATCT
BsaHI 1590 GGCGTC G[GA]^CG[CT]C
5865 GACGTC
BsmI 6099 GAATGC GAATGC(1/-1)
5877 GCATTC
BspLU11I 4634 ACATGT A^CATGT
5633 ACATGT
BstXI 5318 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5805 CCAAAGCTCTGG
Bsu36I 1638 CCTGAGG CC^T[ACGT]AGG
5127 CCTCAGG
DrdI 2469 GACGGATGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2962 GACCATTTAGTC
Eco81I 1638 CCTGAGG CC^T[ACGT]AGG
5127 CCTCAGG
EcoNI 2738 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6385 CCTGACACAGG
Hin1I 1590 GGCGTC G[GA]^CG[CT]C
5865 GACGTC
PinAI 2085 ACCGGT A^CCGGT
5012 ACCGGT
ScaI 3727 AGTACT AGT^ACT
5381 AGTACT
SnaBI 4275 TACGTA TAC^GTA
6323 TACGTA
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BamHI BanI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflFI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII