##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AgeI AhdI Aor51HI
AscI Asp718I AspEI AviII AvrII
BbvCI BlnI BsaBI BspDI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FseI FspI HpaI
KpnI KspI MamI MluI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PstI
PvuI RsrII SacII SalI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1621 ACACGT A^C[GA][CT]GT
AseI 272 ATTAAT AT^TAAT
AsnI 272 ATTAAT AT^TAAT
BbeI 6058 GGCGCC GGCGC^C
BbrPI 5217 CACGTG CAC^GTG
BsiEI 5997 CGACCG CG[GA][CT]^CG
BsiWI 5718 CGTACG C^GTACG
BsmBI 1012 GAGACG CGTCTC(1/5)
BstAPI 1086 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 5436 GACATCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2685 GAATTC G^AATTC
EcoT22I 6176 ATGCAT ATGCA^T
KasI 6058 GGCGCC G^GCGCC
MfeI 256 CAATTG C^AATTG
MunI 256 CAATTG C^AATTG
NarI 6058 GGCGCC GG^CGCC
NdeI 4207 CATATG CA^TATG
NsiI 6176 ATGCAT ATGCA^T
PmaCI 5217 CACGTG CAC^GTG
PmlI 5217 CACGTG CAC^GTG
PshBI 272 ATTAAT AT^TAAT
SexAI 391 ACCTGGT A^CC[AT]GGT
SfiI 529 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 6058 GGCGCC GGC^GCC
SmaI 5872 CCCGGG CCC^GGG
SphI 2308 GCATGC GCATG^C
XmaCI 5872 CCCGGG C^CCGGG
XmaI 5872 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 53 enzymes) #
###################################
AccI 2796 GTCTAC GT^[AC][GT]AC
3402 GTCTAC
AccII 2499 CGCG CG^CG
2552 CGCG
AccIII 3067 TCCGGA T^CCGGA
4980 TCCGGA
AclI 3624 AACGTT AA^CGTT
5031 AACGTT
AcyI 2638 GACGCC G[GA]^CG[CT]C
6058 GGCGCC
AflII 3932 CTTAAG C^TTAAG
4032 CTTAAG
Alw44I 780 GTGCAC G^TGCAC
1259 GTGCAC
AocI 3876 CCTCAGG CC^T[ACGT]AGG
4695 CCTCAGG
ApaLI 780 GTGCAC G^TGCAC
1259 GTGCAC
AvaI 4882 CCCGAG C^[CT]CG[GA]G
5872 CCCGGG
BalI 908 TGGCCA TGG^CCA
4163 TGGCCA
BcgI 3681 CGAAGCATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1600 GCAGGATCTTCG
BfrI 3932 CTTAAG C^TTAAG
4032 CTTAAG
BglI 530 GCCCAAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3285 GCCCTTGGGGC
BlpI 561 GCTCAGC GC^T[ACGT]AGC
5565 GCTGAGC
Bpu1102I 561 GCTCAGC GC^T[ACGT]AGC
5565 GCTGAGC
BsaAI 1403 CACGTA [CT]AC^GT[GA]
5217 CACGTG
BsaHI 2638 GACGCC G[GA]^CG[CT]C
6058 GGCGCC
BseAI 3067 TCCGGA T^CCGGA
4980 TCCGGA
BsoBI 4882 CCCGAG C^[CT]CG[GA]G
5872 CCCGGG
Bsp1407I 4099 TGTACA T^GTACA
4504 TGTACA
BspEI 3067 TCCGGA T^CCGGA
4980 TCCGGA
BsrBI 440 GAGCGG CCGCTC(-3/-3)
1275 GAGCGG
BsrDI 1091 CATTGC GCAATG(2/0)
6210 GCAATG
BsrFI 1372 ACCGGC [GA]^CCGG[CT]
6061 GCCGGT
BsrGI 4099 TGTACA T^GTACA
4504 TGTACA
BstEII 1106 GGTGACC G^GT[ACGT]ACC
1916 GGTCACC
BstPI 1106 GGTGACC G^GT[ACGT]ACC
1916 GGTCACC
BstUI 2499 CGCG CG^CG
2552 CGCG
Bsu36I 3876 CCTCAGG CC^T[ACGT]AGG
4695 CCTCAGG
CelII 561 GCTCAGC GC^T[ACGT]AGC
5565 GCTGAGC
Cfr10I 1372 ACCGGC [GA]^CCGG[CT]
6061 GCCGGT
DraI 3270 TTTAAA TTT^AAA
4554 TTTAAA
DraIII 2884 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3250 CACACGGTG
Eco81I 3876 CCTCAGG CC^T[ACGT]AGG
4695 CCTCAGG
EcoNI 2697 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2753 CCTGACTGAGG
EcoO65I 1106 GGTGACC G^GT[ACGT]ACC
1916 GGTCACC
FauI 5054 CCCGC CCCGC(4/6)
442 GCGGG
Hin1I 2638 GACGCC G[GA]^CG[CT]C
6058 GGCGCC
HindIII 3735 AAGCTT A^AGCTT
5979 AAGCTT
MluNI 908 TGGCCA TGG^CCA
4163 TGGCCA
MroI 3067 TCCGGA T^CCGGA
4980 TCCGGA
MscI 908 TGGCCA TGG^CCA
4163 TGGCCA
MvnI 2499 CGCG CG^CG
2552 CGCG
NcoI 3118 CCATGG C^CATGG
5782 CCATGG
PpuMI 301 AGGACCT [GA]G^G[AT]CC[CT]
5502 AGGTCCT
PshAI 2766 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6624 GACAAATGTC
Psp1406I 3624 AACGTT AA^CGTT
5031 AACGTT
PvuII 564 CAGCTG CAG^CTG
5004 CAGCTG
SacI 798 GAGCTC GAGCT^C
1560 GAGCTC
ScaI 4969 AGTACT AGT^ACT
5356 AGTACT
SspBI 4099 TGTACA T^GTACA
4504 TGTACA
SspI 1184 AATATT AAT^ATT
5256 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AspI AvaII BamHI BanI BanII
BbsI BbvI BciVI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspMI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmnI