##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AclI AflII AscI
AspI AviII BbrPI BclI BfrI
BseAI BsiWI BspDI BspEI BspHI
Bst1107I BstBI BstZ17I ClaI CpoI
EcoT22I FbaI FseI FspI HpaI
MluI MroI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AgeI 2211 ACCGGT A^CCGGT
AhdI 6405 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 430 AGCGCT AGC^GCT
AspEI 6405 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4803 GGATCC G^GATCC
BcgI 182 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 5890 AGATCT A^GATCT
BsaBI 3206 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1114 GAGACG CGTCTC(1/5)
Bsp1407I 6572 TGTACA T^GTACA
BspLU11I 3075 ACATGT A^CATGT
BsrBI 4425 CCGCTC CCGCTC(-3/-3)
BsrGI 6572 TGTACA T^GTACA
BstEII 2069 GGTAACC G^GT[ACGT]ACC
BstPI 2069 GGTAACC G^GT[ACGT]ACC
DraIII 2124 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3669 GACCACCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6405 GACACGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 430 AGCGCT AGC^GCT
EcoO65I 2069 GGTAACC G^GT[ACGT]ACC
EcoRI 6566 GAATTC G^AATTC
EcoRV 2901 GATATC GAT^ATC
HincII 3789 GTTGAC GT[CT]^[GA]AC
HindII 3789 GTTGAC GT[CT]^[GA]AC
KspI 5044 CCGCGG CCGC^GG
MamI 3206 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 4479 CCATGG C^CATGG
NheI 2511 GCTAGC G^CTAGC
PinAI 2211 ACCGGT A^CCGGT
PshAI 5252 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 5044 CCGCGG CCGC^GG
SapI 2506 GAAGAGC GCTCTTC(1/4)
ScaI 6502 AGTACT AGT^ACT
SfiI 876 GGCCACAACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 6499 ACTAGT A^CTAGT
SspBI 6572 TGTACA T^GTACA
XbaI 6578 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 6090 GGTACC G^GTACC
6283 GGTACC
AflIII 3075 ACATGT A^C[GA][CT]GT
4412 ACGTGT
AocI 796 CCTCAGG CC^T[ACGT]AGG
811 CCTCAGG
AseI 2948 ATTAAT AT^TAAT
3717 ATTAAT
AsnI 2948 ATTAAT AT^TAAT
3717 ATTAAT
Asp700I 81 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
225 GAACCAGTTC
Asp718I 6090 GGTACC G^GTACC
6283 GGTACC
AvrII 3067 CCTAGG C^CTAGG
5788 CCTAGG
BglI 615 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
877 GCCACAACGGC
BlnI 3067 CCTAGG C^CTAGG
5788 CCTAGG
BlpI 973 GCTGAGC GC^T[ACGT]AGC
4773 GCTCAGC
Bpu1102I 973 GCTGAGC GC^T[ACGT]AGC
4773 GCTCAGC
BsrDI 345 CATTGC GCAATG(2/0)
3779 GCAATG
BssSI 6033 CACGAG CACGAG(-5/-1)
6149 CACGAG
BstAPI 207 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
280 GCACTGCCTGC
Bsu36I 796 CCTCAGG CC^T[ACGT]AGG
811 CCTCAGG
CelII 973 GCTGAGC GC^T[ACGT]AGC
4773 GCTCAGC
Eco81I 796 CCTCAGG CC^T[ACGT]AGG
811 CCTCAGG
KpnI 6090 GGTACC GGTAC^C
6283 GGTACC
MfeI 1037 CAATTG C^AATTG
1752 CAATTG
MunI 1037 CAATTG C^AATTG
1752 CAATTG
PshBI 2948 ATTAAT AT^TAAT
3717 ATTAAT
SexAI 2125 ACCAGGT A^CC[AT]GGT
3845 ACCAGGT
XmnI 81 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
225 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI