##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AflII Aor51HI ApaI
AscI AseI AsnI AspI AviII
AvrII BbeI BcgI BciVI BclI
BfrI BlnI BsaBI BsiWI BsmBI
BspDI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI Eco47III EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
MamI MfeI MluI MunI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshBI
Psp1406I PvuI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 775 GTCTAC GT^[AC][GT]AC
AccIII 2092 TCCGGA T^CCGGA
AcsI 28 AAATTT [GA]^AATT[CT]
AcyI 1857 GACGCC G[GA]^CG[CT]C
AgeI 1250 ACCGGT A^CCGGT
AhdI 1717 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2338 GTGCAC G^TGCAC
ApaLI 2338 GTGCAC G^TGCAC
ApoI 28 AAATTT [GA]^AATT[CT]
AspEI 1717 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2428 TGGCCA TGG^CCA
BbvCI 1897 GCTGAGG CCTCAGC(-5/-2)
BfaI 1363 CTAG C^TAG
BsaHI 1857 GACGCC G[GA]^CG[CT]C
BseAI 2092 TCCGGA T^CCGGA
BsgI 1152 GTGCAG GTGCAG(16/14)
BsmI 309 GCATTC GAATGC(1/-1)
Bsp1407I 1967 TGTACA T^GTACA
BspEI 2092 TCCGGA T^CCGGA
BspHI 419 TCATGA T^CATGA
BspLU11I 86 ACATGT A^CATGT
BsrBI 1092 CCGCTC CCGCTC(-3/-3)
BsrDI 1996 GCAATG GCAATG(2/0)
BsrGI 1967 TGTACA T^GTACA
BstXI 1562 CCACTCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 518 CGGACCG CG^G[AT]CCG
DraIII 460 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2559 GACTATAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 958 CGGCCG C^GGCCG
Eam1105I 1717 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 958 CGGCCG C^GGCCG
Eco52I 958 CGGCCG C^GGCCG
EcoNI 1125 CCTCAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 112 GATATC GAT^ATC
HaeII 1447 AGCGCC [GA]GCGC^[CT]
Hin1I 1857 GACGCC G[GA]^CG[CT]C
KspI 760 CCGCGG CCGC^GG
MaeI 1363 CTAG C^TAG
MluNI 2428 TGGCCA TGG^CCA
MroI 2092 TCCGGA T^CCGGA
MscI 2428 TGGCCA TGG^CCA
NaeI 361 GCCGGC GCC^GGC
NgoMIV 361 GCCGGC G^CCGGC
PflMI 1590 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1250 ACCGGT A^CCGGT
PshAI 2078 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 419 TCATGA T^CATGA
RsrII 518 CGGACCG CG^G[AT]CCG
SacI 2046 GAGCTC GAGCT^C
SacII 760 CCGCGG CCGC^GG
ScaI 1241 AGTACT AGT^ACT
SmaI 569 CCCGGG CCC^GGG
SphI 425 GCATGC GCATG^C
SspBI 1967 TGTACA T^GTACA
Van91I 1590 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 569 CCCGGG C^CCGGG
XmaI 569 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 288 GGTACC G^GTACC
2161 GGTACC
AflIII 86 ACATGT A^C[GA][CT]GT
1148 ACACGT
AocI 814 CCTAAGG CC^T[ACGT]AGG
1125 CCTCAGG
Asp700I 399 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1311 GAAGCCGTTC
Asp718I 288 GGTACC G^GTACC
2161 GGTACC
BamHI 885 GGATCC G^GATCC
1116 GGATCC
BbrPI 1149 CACGTG CAC^GTG
1285 CACGTG
BbsI 46 GAAGAC GAAGAC(2/6)
2017 GAAGAC
BglI 1 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1033 GCCATGAAGGC
BglII 1169 AGATCT A^GATCT
1180 AGATCT
BpuAI 46 GAAGAC GAAGAC(2/6)
2017 GAAGAC
BsaAI 1149 CACGTG [CT]AC^GT[GA]
1285 CACGTG
BsaI 2497 GAGACC GGTCTC(1/5)
2403 GGTCTC
BsaWI 1250 ACCGGT [AT]^CCGG[AT]
2092 TCCGGA
BsiEI 125 CGACCG CG[GA][CT]^CG
958 CGGCCG
BspMI 953 ACCTGC ACCTGC(4/8)
1154 GCAGGT
BssSI 1390 CACGAG CACGAG(-5/-1)
2386 CTCGTG
Bsu36I 814 CCTAAGG CC^T[ACGT]AGG
1125 CCTCAGG
CfoI 1397 GCGC GCG^C
1448 GCGC
Eco81I 814 CCTAAGG CC^T[ACGT]AGG
1125 CCTCAGG
FauI 321 CCCGC CCCGC(4/6)
646 CCCGC
HhaI 1397 GCGC GCG^C
1448 GCGC
HinP1I 1397 GCGC G^CGC
1448 GCGC
KpnI 288 GGTACC GGTAC^C
2161 GGTACC
NcoI 1790 CCATGG C^CATGG
2371 CCATGG
NdeI 53 CATATG CA^TATG
1991 CATATG
PmaCI 1149 CACGTG CAC^GTG
1285 CACGTG
PmlI 1149 CACGTG CAC^GTG
1285 CACGTG
TaqI 614 TCGA T^CGA
1566 TCGA
Tsp509I 29 AATT ^AATT
1930 AATT
TthHB8I 614 TCGA T^CGA
1566 TCGA
XmnI 399 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1311 GAAGCCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I TspRI XcmI XhoII