##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BfrI BlnI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FspI KpnI MfeI MluI MroI
MunI NdeI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SfiI SfuI SnaBI
SpeI SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 7004 GACGTC GACGT^C
AcsI 7577 AAATTT [GA]^AATT[CT]
ApoI 7577 AAATTT [GA]^AATT[CT]
AspI 7073 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2674 GGATCC G^GATCC
BbrPI 5060 CACGTG CAC^GTG
BcgI 2985 GCAGGAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 7535 TGATCA T^GATCA
BsaAI 5060 CACGTG [CT]AC^GT[GA]
BsaBI 2113 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 7002 GAGACG CGTCTC(1/5)
BspHI 326 TCATGA T^CATGA
BssSI 898 CACGAG CACGAG(-5/-1)
BstEII 5050 GGTGACC G^GT[ACGT]ACC
BstPI 5050 GGTGACC G^GT[ACGT]ACC
DraIII 5403 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 5050 GGTGACC G^GT[ACGT]ACC
FbaI 7535 TGATCA T^GATCA
HpaI 7290 GTTAAC GTT^AAC
MamI 2113 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 207 GCGGCCGC GC^GGCCGC
PaeR7I 2129 CTCGAG C^TCGAG
PflFI 7073 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5060 CACGTG CAC^GTG
PmlI 5060 CACGTG CAC^GTG
PshAI 5281 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 326 TCATGA T^CATGA
SalI 702 GTCGAC G^TCGAC
ScaI 5019 AGTACT AGT^ACT
SexAI 649 ACCTGGT A^CC[AT]GGT
SgrAI 160 CGCCGGTG C[GA]^CCGG[CT]G
Tth111I 7073 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2129 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 5002 GTGCAC G^TGCAC
5838 GTGCAC
ApaLI 5002 GTGCAC G^TGCAC
5838 GTGCAC
BbeI 264 GGCGCC GGCGC^C
3001 GGCGCC
BciVI 995 GTATCC GTATCC(6/5)
6387 GTATCC
BsrBI 78 GAGCGG CCGCTC(-3/-3)
5139 GAGCGG
BssHII 213 GCGCGC G^CGCGC
215 GCGCGC
DraI 6941 TTTAAA TTT^AAA
7631 TTTAAA
FseI 2456 GGCCGGCC GGCCGG^CC
5595 GGCCGGCC
HincII 702 GTCGAC GT[CT]^[GA]AC
7290 GTTAAC
HindII 702 GTCGAC GT[CT]^[GA]AC
7290 GTTAAC
KasI 264 GGCGCC G^GCGCC
3001 GGCGCC
KspI 1305 CCGCGG CCGC^GG
5533 CCGCGG
NarI 264 GGCGCC GG^CGCC
3001 GGCGCC
SacII 1305 CCGCGG CCGC^GG
5533 CCGCGG
SbfI 4641 CCTGCAGG CCTGCA^GG
6749 CCTGCAGG
SfoI 264 GGCGCC GGC^GCC
3001 GGCGCC
SmaI 2910 CCCGGG CCC^GGG
4609 CCCGGG
Sse8387I 4641 CCTGCAGG CCTGCA^GG
6749 CCTGCAGG
XbaI 24 TCTAGA T^CTAGA
4879 TCTAGA
XmaCI 2910 CCCGGG C^CCGGG
4609 CCCGGG
XmaI 2910 CCCGGG C^CCGGG
4609 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII