################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII Acc65I AcyI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AviII BbeI BcgI BlpI Bpu1102I BsaBI BsaHI BsiEI BsiWI BspDI BssHII CelII ClaI DrdI EagI Eam1105I EclXI Eco47III Eco52I FseI FspI Hin1I KasI KpnI MamI NaeI NarI NgoMIV NheI NotI NruI PacI PvuI SalI SbfI SexAI SfiI SfoI SgrAI SpeI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 1028 GTATAC GT^[AC][GT]AC AccIII 2357 TCCGGA T^CCGGA AgeI 1903 ACCGGT A^CCGGT ApaI 1698 GGGCCC GGGCC^C BalI 2444 TGGCCA TGG^CCA BanI 3324 GGCACC G^G[CT][GA]CC BciVI 5919 GGATAC GTATCC(6/5) BclI 1957 TGATCA T^GATCA BglI 3054 GCCAACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 5986 AGATCT A^GATCT BseAI 2357 TCCGGA T^CCGGA BsmBI 6713 CGTCTC CGTCTC(1/5) BspEI 2357 TCCGGA T^CCGGA BsrDI 7067 CATTGC GCAATG(2/0) BssSI 486 CACGAG CACGAG(-5/-1) Bst1107I 1028 GTATAC GTA^TAC BstBI 5363 TTCGAA TT^CGAA BstEII 756 GGTGACC G^GT[ACGT]ACC BstPI 756 GGTGACC G^GT[ACGT]ACC BstZ17I 1028 GTATAC GTA^TAC CpoI 1900 CGGACCG CG^G[AT]CCG EaeI 2444 TGGCCA [CT]^GGCC[GA] EcoO65I 756 GGTGACC G^GT[ACGT]ACC EcoRV 2904 GATATC GAT^ATC EcoT22I 2752 ATGCAT ATGCA^T FbaI 1957 TGATCA T^GATCA KspI 145 CCGCGG CCGC^GG MfeI 286 CAATTG C^AATTG MluI 1820 ACGCGT A^CGCGT MluNI 2444 TGGCCA TGG^CCA MroI 2357 TCCGGA T^CCGGA MscI 2444 TGGCCA TGG^CCA MunI 286 CAATTG C^AATTG NsiI 2752 ATGCAT ATGCA^T NspV 5363 TTCGAA TT^CGAA PinAI 1903 ACCGGT A^CCGGT PmeI 5798 GTTTAAAC GTTT^AAAC RsrII 1900 CGGACCG CG^G[AT]CCG SacII 145 CCGCGG CCGC^GG SfuI 5363 TTCGAA TT^CGAA SnaBI 4260 TACGTA TAC^GTA SspI 5781 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AclI 4902 AACGTT AA^CGTT 7521 AACGTT AocI 5570 CCTGAGG CC^T[ACGT]AGG 6660 CCTTAGG AseI 5219 ATTAAT AT^TAAT 7723 ATTAAT AsnI 5219 ATTAAT AT^TAAT 7723 ATTAAT BbrPI 6147 CACGTG CAC^GTG 7447 CACGTG BsaI 996 GGTCTC GGTCTC(1/5) 3916 GGTCTC Bsp1407I 2259 TGTACA T^GTACA 3421 TGTACA BsrGI 2259 TGTACA T^GTACA 3421 TGTACA Bsu36I 5570 CCTGAGG CC^T[ACGT]AGG 6660 CCTTAGG Eco81I 5570 CCTGAGG CC^T[ACGT]AGG 6660 CCTTAGG HpaI 263 GTTAAC GTT^AAC 2202 GTTAAC NdeI 4132 CATATG CA^TATG 6414 CATATG PaeR7I 499 CTCGAG C^TCGAG 1544 CTCGAG PflMI 1704 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4736 CCATACAGTGG PmaCI 6147 CACGTG CAC^GTG 7447 CACGTG PmlI 6147 CACGTG CAC^GTG 7447 CACGTG PshBI 5219 ATTAAT AT^TAAT 7723 ATTAAT Psp1406I 4902 AACGTT AA^CGTT 7521 AACGTT SmaI 716 CCCGGG CCC^GGG 3300 CCCGGG SspBI 2259 TGTACA T^GTACA 3421 TGTACA Van91I 1704 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4736 CCATACAGTGG XbaI 3143 TCTAGA T^CTAGA 5989 TCTAGA XcmI 4736 CCATACAGTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5295 CCAAGTCAGTGATGG XhoI 499 CTCGAG C^TCGAG 1544 CTCGAG XmaCI 716 CCCGGG C^CCGGG 3300 CCCGGG XmaI 716 CCCGGG C^CCGGG 3300 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AspI AvaI AvaII AvrII BamHI BanII BbsI BbvCI BbvI BcnI BfaI BfrI BlnI BmyI BpmI BpuAI BsaAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PshAI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmnI