##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
ApaLI AscI AviII BbrPI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoO65I EcoRV FseI
FspI KspI MfeI MluI MroI
MunI NotI NruI PacI PaeR7I
PinAI PmaCI PmlI Psp1406I PvuI
RsrII SacII SalI SfiI SgrAI
SmaI SspBI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 5819 GGTACC G^GTACC
AccII 1738 CGCG CG^CG
AflII 4550 CTTAAG C^TTAAG
AflIII 1334 ACATGT A^C[GA][CT]GT
AhdI 6481 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3328 AGCGCT AGC^GCT
Asp718I 5819 GGTACC G^GTACC
AspEI 6481 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 6805 CCTAGG C^CTAGG
BcgI 7183 CGAACATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4550 CTTAAG C^TTAAG
BlnI 6805 CCTAGG C^CTAGG
BsaBI 4590 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 8194 GCATTC GAATGC(1/-1)
BspHI 3848 TCATGA T^CATGA
BspLU11I 1334 ACATGT A^CATGT
BstAPI 6351 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 7181 TTCGAA TT^CGAA
BstUI 1738 CGCG CG^CG
Eam1105I 6481 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3328 AGCGCT AGC^GCT
EcoT22I 2157 ATGCAT ATGCA^T
HindIII 1209 AAGCTT A^AGCTT
HpaI 5304 GTTAAC GTT^AAC
KpnI 5819 GGTACC GGTAC^C
MamI 4590 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1738 CGCG CG^CG
NsiI 2157 ATGCAT ATGCA^T
NspV 7181 TTCGAA TT^CGAA
PmeI 5363 GTTTAAAC GTTT^AAAC
PshAI 7585 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3848 TCATGA T^CATGA
SapI 202 GAAGAGC GCTCTTC(1/4)
ScaI 5913 AGTACT AGT^ACT
SfuI 7181 TTCGAA TT^CGAA
SnaBI 3888 TACGTA TAC^GTA
SspI 7082 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AseI 3866 ATTAAT AT^TAAT
6598 ATTAAT
AsnI 3866 ATTAAT AT^TAAT
6598 ATTAAT
Asp700I 4792 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7566 GAAACAGTTC
AspI 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6662 GACTTTGTC
AvaI 4536 CCCGAG C^[CT]CG[GA]G
6207 CTCGGG
BamHI 1672 GGATCC G^GATCC
5129 GGATCC
BbeI 606 GGCGCC GGCGC^C
885 GGCGCC
BbvCI 1304 CCTCAGC CCTCAGC(-5/-2)
6948 CCTCAGC
BglII 2023 AGATCT A^GATCT
3657 AGATCT
BsaWI 996 TCCGGT [AT]^CCGG[AT]
3143 TCCGGT
BsoBI 4536 CCCGAG C^[CT]CG[GA]G
6207 CTCGGG
BsrBI 595 CCGCTC CCGCTC(-3/-3)
1003 CCGCTC
BsrDI 5594 CATTGC GCAATG(2/0)
6362 CATTGC
EcoRI 1924 GAATTC G^AATTC
5421 GAATTC
KasI 606 GGCGCC G^GCGCC
885 GGCGCC
NaeI 978 GCCGGC GCC^GGC
5136 GCCGGC
NarI 606 GGCGCC GG^CGCC
885 GGCGCC
NdeI 2233 CATATG CA^TATG
3997 CATATG
NgoMIV 978 GCCGGC G^CCGGC
5136 GCCGGC
NheI 583 GCTAGC G^CTAGC
2622 GCTAGC
PflFI 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6662 GACTTTGTC
PshBI 3866 ATTAAT AT^TAAT
6598 ATTAAT
SbfI 1316 CCTGCAGG CCTGCA^GG
2011 CCTGCAGG
SexAI 2268 ACCAGGT A^CC[AT]GGT
3633 ACCAGGT
SfoI 606 GGCGCC GGC^GCC
885 GGCGCC
SpeI 1220 ACTAGT A^CTAGT
4023 ACTAGT
SphI 2002 GCATGC GCATG^C
6315 GCATGC
Sse8387I 1316 CCTGCAGG CCTGCA^GG
2011 CCTGCAGG
Tth111I 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6662 GACTTTGTC
XbaI 3212 TCTAGA T^CTAGA
4586 TCTAGA
XmnI 4792 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7566 GAAACAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaII BalI BanI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII