##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AscI AseI
AsnI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BciVI BfrI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DraIII
DrdI EagI EclXI Eco52I EcoO65I
EcoRI EcoT22I FseI FspI Hin1I
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 1762 AAATTT [GA]^AATT[CT]
AhdI 386 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 857 GTGCAC G^TGCAC
AlwNI 1104 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 118 AGCGCT AGC^GCT
ApaLI 857 GTGCAC G^TGCAC
ApoI 1762 AAATTT [GA]^AATT[CT]
Asp700I 457 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 386 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 10 GGATCC G^GATCC
BclI 495 TGATCA T^GATCA
BglI 1615 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1559 AGATCT A^GATCT
BsgI 1154 GTGCAG GTGCAG(16/14)
BsiEI 792 CGATCG CG[GA][CT]^CG
BsmI 1872 GAATGC GAATGC(1/-1)
BspDI 794 ATCGAT AT^CGAT
BspHI 1227 TCATGA T^CATGA
ClaI 794 ATCGAT AT^CGAT
DraI 1759 TTTAAA TTT^AAA
Eam1105I 386 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1656 GAAGAG CTCTTC(1/4)
Eco47III 118 AGCGCT AGC^GCT
EcoRV 210 GATATC GAT^ATC
FbaI 495 TGATCA T^GATCA
HaeII 118 AGCGCT [GA]GCGC^[CT]
HgaI 107 GACGC GACGC(5/10)
HincII 641 GTCAAC GT[CT]^[GA]AC
HindII 641 GTCAAC GT[CT]^[GA]AC
Ksp632I 1656 GAAGAG CTCTTC(1/4)
MaeII 980 ACGT A^CGT
MseI 1760 TTAA T^TAA
PvuI 792 CGATCG CGAT^CG
RcaI 1227 TCATGA T^CATGA
SapI 1656 GAAGAGC GCTCTTC(1/4)
SbfI 538 CCTGCAGG CCTGCA^GG
Sse8387I 538 CCTGCAGG CCTGCA^GG
StuI 1647 AGGCCT AGG^CCT
TfiI 1248 GAATC G^A[AT]TC
Tru9I 1760 TTAA T^TAA
XcmI 1272 CCATCGTCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 457 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 176 GGTACC G^GTACC
2368 GGTACC
ApaI 1091 GGGCCC GGGCC^C
1565 GGGCCC
Asp718I 176 GGTACC G^GTACC
2368 GGTACC
BalI 895 TGGCCA TGG^CCA
1596 TGGCCA
BlpI 1309 GCTCAGC GC^T[ACGT]AGC
2200 GCTGAGC
Bpu1102I 1309 GCTCAGC GC^T[ACGT]AGC
2200 GCTGAGC
BsaI 393 GGTCTC GGTCTC(1/5)
938 GGTCTC
BsrBI 250 CCGCTC CCGCTC(-3/-3)
1353 GAGCGG
BstAPI 1103 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2032 GCATGAGCTGC
BstXI 1125 CCAATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2155 CCACTGGCTTGG
CelII 1309 GCTCAGC GC^T[ACGT]AGC
2200 GCTGAGC
KpnI 176 GGTACC GGTAC^C
2368 GGTACC
MluNI 895 TGGCCA TGG^CCA
1596 TGGCCA
MscI 895 TGGCCA TGG^CCA
1596 TGGCCA
NcoI 240 CCATGG C^CATGG
1131 CCATGG
PaeR7I 1336 CTCGAG C^TCGAG
1693 CTCGAG
PflMI 1952 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2206 CCAAGCCCTGG
SacI 689 GAGCTC GAGCT^C
1171 GAGCTC
SmaI 353 CCCGGG CCC^GGG
2022 CCCGGG
Van91I 1952 CCACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2206 CCAAGCCCTGG
XhoI 1336 CTCGAG C^TCGAG
1693 CTCGAG
XmaCI 353 CCCGGG C^CCGGG
2022 CCCGGG
XmaI 353 CCCGGG C^CCGGG
2022 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI AspHI AvaI AvaII BanI
BanII BbvI BcgI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII