##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BcgI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsgI BsiWI BsoBI BspDI BspEI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI Eam1105I
Eco81I EcoNI EcoO65I EcoRV FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI NaeI NarI NgoMIV
NheI NruI NspV PacI PaeR7I
PinAI PmaCI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1792 GTAGAC GT^[AC][GT]AC
AccII 34 CGCG CG^CG
AcyI 222 GACGCC G[GA]^CG[CT]C
AflIII 2633 ACATGT A^C[GA][CT]GT
Aor51HI 1318 AGCGCT AGC^GCT
AspI 1835 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2347 GGTGCC G^G[CT][GA]CC
BbsI 1312 GTCTTC GAAGAC(2/6)
BbvCI 2002 GCTGAGG CCTCAGC(-5/-2)
BcnI 71 CCGGG CC^[GC]GG
BglII 261 AGATCT A^GATCT
BpuAI 1312 GTCTTC GAAGAC(2/6)
BsaHI 222 GACGCC G[GA]^CG[CT]C
BsaWI 130 ACCGGA [AT]^CCGG[AT]
BseRI 1303 CTCCTC GAGGAG(10/8)
BsmBI 220 GAGACG CGTCTC(1/5)
BsmI 1403 GAATGC GAATGC(1/-1)
BspHI 2202 TCATGA T^CATGA
BspLU11I 2633 ACATGT A^CATGT
BspMI 1418 ACCTGC ACCTGC(4/8)
BsrBI 39 CCGCTC CCGCTC(-3/-3)
BsrFI 2675 GCCGGT [GA]^CCGG[CT]
BstAPI 1377 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 34 CGCG CG^CG
Cfr10I 2675 GCCGGT [GA]^CCGG[CT]
DraII 844 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1953 CCATGG C^C[GA][CT]GG
EarI 643 GAAGAG CTCTTC(1/4)
Eco47III 1318 AGCGCT AGC^GCT
EcoO109I 844 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1694 GAATTC G^AATTC
HgaI 222 GACGC GACGC(5/10)
Hin1I 222 GACGCC G[GA]^CG[CT]C
HindIII 958 AAGCTT A^AGCTT
Ksp632I 643 GAAGAG CTCTTC(1/4)
MfeI 524 CAATTG C^AATTG
MunI 524 CAATTG C^AATTG
MvnI 34 CGCG CG^CG
NciI 71 CCGGG CC^[GC]GG
NcoI 1953 CCATGG C^CATGG
NdeI 1557 CATATG CA^TATG
NotI 35 GCGGCCGC GC^GGCCGC
PflFI 1835 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 2637 GTTTAAAC GTTT^AAAC
PpuMI 844 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1234 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2202 TCATGA T^CATGA
SexAI 1346 ACCTGGT A^CC[AT]GGT
SpeI 669 ACTAGT A^CTAGT
SspI 2734 AATATT AAT^ATT
Tth111I 1835 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 321 GTGCAC G^TGCAC
394 GTGCAC
ApaLI 321 GTGCAC G^TGCAC
394 GTGCAC
Asp700I 1694 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2847 GAAGCACTTC
BciVI 1642 GGATAC GTATCC(6/5)
2165 GGATAC
BpmI 697 CTCCAG CTGGAG(16/14)
1216 CTCCAG
BsaJI 1953 CCATGG C^C[ACGT][ACGT]GG
2309 CCTTGG
BstXI 558 CCAAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3014 CCAGAAACATGG
EaeI 36 CGGCCG [CT]^GGCC[GA]
2673 CGGCCG
EagI 36 CGGCCG C^GGCCG
2673 CGGCCG
EclXI 36 CGGCCG C^GGCCG
2673 CGGCCG
Eco52I 36 CGGCCG C^GGCCG
2673 CGGCCG
EcoT14I 1953 CCATGG C^C[AT][AT]GG
2309 CCTTGG
EcoT22I 1375 ATGCAT ATGCA^T
1405 ATGCAT
HaeII 1318 AGCGCT [GA]GCGC^[CT]
2142 AGCGCC
MspA1I 946 CAGCTG C[AC]G^C[GT]G
2886 CAGCTG
NsiI 1375 ATGCAT ATGCA^T
1405 ATGCAT
NspI 1475 GCATGT [GA]CATG^[CT]
2633 ACATGT
PflMI 1341 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1538 CCATAATATGG
PvuII 946 CAGCTG CAG^CTG
2886 CAGCTG
StyI 1953 CCATGG C^C[AT][AT]GG
2309 CCTTGG
TfiI 468 GATTC G^A[AT]TC
2707 GATTC
Van91I 1341 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1538 CCATAATATGG
XmnI 1694 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2847 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaII
BbvI BclI BfaI BfrI BmyI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII