##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BamHI BbrPI
BcgI BfaI BlnI BlpI Bpu1102I
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI DraI EaeI
EagI EclXI Eco52I Eco81I EcoRI
EcoRV FseI FspI HindIII KpnI
MaeI MfeI MluI MluNI MroI
MscI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1074 GACGTC GACGT^C
AhdI 1424 GACGAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1537 AGCGCT AGC^GCT
Asp700I 1341 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1424 GACGAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2055 GAGCTC G[AT]GC[AT]^C
BanI 85 GGCGCC G^G[CT][GA]CC
BbeI 85 GGCGCC GGCGC^C
BciVI 588 GTATCC GTATCC(6/5)
BglI 2194 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 266 AGATCT A^GATCT
BsaAI 1740 CACGTA [CT]AC^GT[GA]
BsaI 720 GAGACC GGTCTC(1/5)
BsaWI 2154 TCCGGT [AT]^CCGG[AT]
BsiHKAI 2055 GAGCTC G[AT]GC[AT]^C
BsmBI 1236 GAGACG CGTCTC(1/5)
BspHI 2238 TCATGA T^CATGA
BspMI 1389 GCAGGT ACCTGC(4/8)
BssHII 659 GCGCGC G^CGCGC
BstAPI 492 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1644 GGTGACC G^GT[ACGT]ACC
BstPI 1644 GGTGACC G^GT[ACGT]ACC
CpoI 2151 CGGTCCG CG^G[AT]CCG
DraII 674 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 2149 GACGGTCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1424 GACGAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1537 AGCGCT AGC^GCT
EcoO109I 674 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1644 GGTGACC G^GT[ACGT]ACC
EcoT22I 969 ATGCAT ATGCA^T
HgaI 369 GACGC GACGC(5/10)
HincII 2560 GTTAAC GT[CT]^[GA]AC
HindII 2560 GTTAAC GT[CT]^[GA]AC
HpaI 2560 GTTAAC GTT^AAC
KasI 85 GGCGCC G^GCGCC
KspI 28 CCGCGG CCGC^GG
NarI 85 GGCGCC GG^CGCC
NcoI 607 CCATGG C^CATGG
NsiI 969 ATGCAT ATGCA^T
NspI 2230 GCATGC [GA]CATG^[CT]
PstI 2028 CTGCAG CTGCA^G
PvuII 2321 CAGCTG CAG^CTG
RcaI 2238 TCATGA T^CATGA
RsrII 2151 CGGTCCG CG^G[AT]CCG
SacI 2055 GAGCTC GAGCT^C
SacII 28 CCGCGG CCGC^GG
SapI 771 GAAGAGC GCTCTTC(1/4)
SexAI 2543 ACCTGGT A^CC[AT]GGT
SfcI 2028 CTGCAG C^T[GA][CT]AG
SfoI 85 GGCGCC GGC^GCC
SphI 2230 GCATGC GCATG^C
SspI 1860 AATATT AAT^ATT
XmnI 1341 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 414 AAATTT [GA]^AATT[CT]
1445 GAATTT
AcyI 85 GGCGCC G[GA]^CG[CT]C
1074 GACGTC
AlwI 1319 GGATC GGATC(4/5)
1593 GGATC
AlwNI 2113 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2192 CAGCCCCTG
ApoI 414 AAATTT [GA]^AATT[CT]
1445 GAATTT
AvaI 1252 CCCGAG C^[CT]CG[GA]G
1368 CTCGGG
BbvCI 1533 CCTCAGC CCTCAGC(-5/-2)
815 GCTGAGG
BclI 344 TGATCA T^GATCA
1159 TGATCA
BsaBI 153 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1763 GATGGACATC
BsaHI 85 GGCGCC G[GA]^CG[CT]C
1074 GACGTC
BsmFI 73 GGGAC GGGAC(10/14)
367 GGGAC
BsoBI 1252 CCCGAG C^[CT]CG[GA]G
1368 CTCGGG
BsrBI 211 CCGCTC CCGCTC(-3/-3)
1427 GAGCGG
BsrFI 723 ACCGGC [GA]^CCGG[CT]
2121 GCCGGT
BstYI 266 AGATCT [GA]^GATC[CT]
1593 GGATCT
Cfr10I 723 ACCGGC [GA]^CCGG[CT]
2121 GCCGGT
EarI 479 GAAGAG CTCTTC(1/4)
771 GAAGAG
EcoNI 510 CCTTCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
666 CCTCACAAAGG
FbaI 344 TGATCA T^GATCA
1159 TGATCA
HaeII 85 GGCGCC [GA]GCGC^[CT]
1537 AGCGCT
Hin1I 85 GGCGCC G[GA]^CG[CT]C
1074 GACGTC
Ksp632I 479 GAAGAG CTCTTC(1/4)
771 GAAGAG
MamI 153 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1763 GATGGACATC
MflI 266 AGATCT [GA]^GATC[CT]
1593 GGATCT
MspA1I 28 CCGCGG C[AC]G^C[GT]G
2321 CAGCTG
NdeI 930 CATATG CA^TATG
1018 CATATG
ScaI 915 AGTACT AGT^ACT
1962 AGTACT
XhoII 266 AGATCT [GA]^GATC[CT]
1593 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AflII AluI
AvaII BanII BbsI BbvI BcnI
BfrI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraIII DsaI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I