################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccI AccIII AclI AflIII AgeI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BbrPI BcgI BfaI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI DraI EaeI EagI EclXI Eco52I Eco81I EcoRI EcoRV FseI FspI HindIII KpnI MaeI MfeI MluI MluNI MroI MscI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 1074 GACGTC GACGT^C AhdI 1424 GACGAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1537 AGCGCT AGC^GCT Asp700I 1341 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1424 GACGAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 2055 GAGCTC G[AT]GC[AT]^C BanI 85 GGCGCC G^G[CT][GA]CC BbeI 85 GGCGCC GGCGC^C BciVI 588 GTATCC GTATCC(6/5) BglI 2194 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 266 AGATCT A^GATCT BsaAI 1740 CACGTA [CT]AC^GT[GA] BsaI 720 GAGACC GGTCTC(1/5) BsaWI 2154 TCCGGT [AT]^CCGG[AT] BsiHKAI 2055 GAGCTC G[AT]GC[AT]^C BsmBI 1236 GAGACG CGTCTC(1/5) BspHI 2238 TCATGA T^CATGA BspMI 1389 GCAGGT ACCTGC(4/8) BssHII 659 GCGCGC G^CGCGC BstAPI 492 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1644 GGTGACC G^GT[ACGT]ACC BstPI 1644 GGTGACC G^GT[ACGT]ACC CpoI 2151 CGGTCCG CG^G[AT]CCG DraII 674 AGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 2149 GACGGTCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1424 GACGAGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1537 AGCGCT AGC^GCT EcoO109I 674 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1644 GGTGACC G^GT[ACGT]ACC EcoT22I 969 ATGCAT ATGCA^T HgaI 369 GACGC GACGC(5/10) HincII 2560 GTTAAC GT[CT]^[GA]AC HindII 2560 GTTAAC GT[CT]^[GA]AC HpaI 2560 GTTAAC GTT^AAC KasI 85 GGCGCC G^GCGCC KspI 28 CCGCGG CCGC^GG NarI 85 GGCGCC GG^CGCC NcoI 607 CCATGG C^CATGG NsiI 969 ATGCAT ATGCA^T NspI 2230 GCATGC [GA]CATG^[CT] PstI 2028 CTGCAG CTGCA^G PvuII 2321 CAGCTG CAG^CTG RcaI 2238 TCATGA T^CATGA RsrII 2151 CGGTCCG CG^G[AT]CCG SacI 2055 GAGCTC GAGCT^C SacII 28 CCGCGG CCGC^GG SapI 771 GAAGAGC GCTCTTC(1/4) SexAI 2543 ACCTGGT A^CC[AT]GGT SfcI 2028 CTGCAG C^T[GA][CT]AG SfoI 85 GGCGCC GGC^GCC SphI 2230 GCATGC GCATG^C SspI 1860 AATATT AAT^ATT XmnI 1341 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 414 AAATTT [GA]^AATT[CT] 1445 GAATTT AcyI 85 GGCGCC G[GA]^CG[CT]C 1074 GACGTC AlwI 1319 GGATC GGATC(4/5) 1593 GGATC AlwNI 2113 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2192 CAGCCCCTG ApoI 414 AAATTT [GA]^AATT[CT] 1445 GAATTT AvaI 1252 CCCGAG C^[CT]CG[GA]G 1368 CTCGGG BbvCI 1533 CCTCAGC CCTCAGC(-5/-2) 815 GCTGAGG BclI 344 TGATCA T^GATCA 1159 TGATCA BsaBI 153 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1763 GATGGACATC BsaHI 85 GGCGCC G[GA]^CG[CT]C 1074 GACGTC BsmFI 73 GGGAC GGGAC(10/14) 367 GGGAC BsoBI 1252 CCCGAG C^[CT]CG[GA]G 1368 CTCGGG BsrBI 211 CCGCTC CCGCTC(-3/-3) 1427 GAGCGG BsrFI 723 ACCGGC [GA]^CCGG[CT] 2121 GCCGGT BstYI 266 AGATCT [GA]^GATC[CT] 1593 GGATCT Cfr10I 723 ACCGGC [GA]^CCGG[CT] 2121 GCCGGT EarI 479 GAAGAG CTCTTC(1/4) 771 GAAGAG EcoNI 510 CCTTCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 666 CCTCACAAAGG FbaI 344 TGATCA T^GATCA 1159 TGATCA HaeII 85 GGCGCC [GA]GCGC^[CT] 1537 AGCGCT Hin1I 85 GGCGCC G[GA]^CG[CT]C 1074 GACGTC Ksp632I 479 GAAGAG CTCTTC(1/4) 771 GAAGAG MamI 153 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1763 GATGGACATC MflI 266 AGATCT [GA]^GATC[CT] 1593 GGATCT MspA1I 28 CCGCGG C[AC]G^C[GT]G 2321 CAGCTG NdeI 930 CATATG CA^TATG 1018 CATATG ScaI 915 AGTACT AGT^ACT 1962 AGTACT XhoII 266 AGATCT [GA]^GATC[CT] 1593 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AfaI AflII AluI AvaII BanII BbsI BbvI BcnI BfrI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraIII DsaI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I