################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AclI Alw44I AocI Aor51HI ApaLI AscI AviII AvrII BbrPI BciVI BglI BlnI BsiEI BsiWI Bst1107I BstZ17I Bsu36I EagI EclXI Eco47III Eco52I Eco81I EcoNI FseI FspI KspI MluI NcoI NheI NotI NruI PacI PmaCI PmeI PmlI Psp1406I PvuI SacII SalI SbfI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 2417 GACGTC GACGT^C Acc65I 6437 GGTACC G^GTACC AgeI 1514 ACCGGT A^CCGGT AhdI 3331 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1658 GGGCCC GGGCC^C Asp718I 6437 GGTACC G^GTACC AspEI 3331 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 3439 GGATCC G^GATCC BbeI 4135 GGCGCC GGCGC^C BclI 3851 TGATCA T^GATCA BlpI 2218 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2218 GCTGAGC GC^T[ACGT]AGC BspLU11I 1914 ACATGT A^CATGT BsrBI 1784 CCGCTC CCGCTC(-3/-3) BssHII 3475 GCGCGC G^CGCGC BstEII 5141 GGTTACC G^GT[ACGT]ACC BstPI 5141 GGTTACC G^GT[ACGT]ACC CelII 2218 GCTGAGC GC^T[ACGT]AGC DsaI 5315 CCACGG C^C[GA][CT]GG Eam1105I 3331 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 5141 GGTTACC G^GT[ACGT]ACC FbaI 3851 TGATCA T^GATCA HaeII 4135 GGCGCC [GA]GCGC^[CT] HindIII 2905 AAGCTT A^AGCTT HpaI 4204 GTTAAC GTT^AAC KasI 4135 GGCGCC G^GCGCC KpnI 6437 GGTACC GGTAC^C NarI 4135 GGCGCC GG^CGCC PinAI 1514 ACCGGT A^CCGGT ScaI 7313 AGTACT AGT^ACT SexAI 2330 ACCTGGT A^CC[AT]GGT SfoI 4135 GGCGCC GGC^GCC SphI 5060 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 1499 TCCGGA T^CCGGA 5265 TCCGGA Asp700I 405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4828 GAACGTATTC AspI 2329 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 8016 GACTCAGTC BglII 7625 AGATCT A^GATCT 7858 AGATCT BseAI 1499 TCCGGA T^CCGGA 5265 TCCGGA BspEI 1499 TCCGGA T^CCGGA 5265 TCCGGA BstBI 1127 TTCGAA TT^CGAA 6465 TTCGAA CpoI 1516 CGGTCCG CG^G[AT]CCG 3001 CGGACCG DraIII 118 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1636 CACGATGTG EcoT22I 876 ATGCAT ATGCA^T 5996 ATGCAT MroI 1499 TCCGGA T^CCGGA 5265 TCCGGA NaeI 646 GCCGGC GCC^GGC 5068 GCCGGC NgoMIV 646 GCCGGC G^CCGGC 5068 GCCGGC NsiI 876 ATGCAT ATGCA^T 5996 ATGCAT NspV 1127 TTCGAA TT^CGAA 6465 TTCGAA PaeR7I 1754 CTCGAG C^TCGAG 5655 CTCGAG PflFI 2329 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 8016 GACTCAGTC PflMI 1319 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4117 CCATTTGATGG PshAI 1247 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 5168 GACTCCTGTC RsrII 1516 CGGTCCG CG^G[AT]CCG 3001 CGGACCG SacI 475 GAGCTC GAGCT^C 2669 GAGCTC SfuI 1127 TTCGAA TT^CGAA 6465 TTCGAA Tth111I 2329 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 8016 GACTCAGTC Van91I 1319 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4117 CCATTTGATGG XhoI 1754 CTCGAG C^TCGAG 5655 CTCGAG XmnI 405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4828 GAACGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 150 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcgI BcnI BfaI BfrI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspMI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraI DraII DrdI EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII