##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI Alw44I AocI Aor51HI ApaLI
AscI AviII AvrII BbrPI BciVI
BglI BlnI BsiEI BsiWI Bst1107I
BstZ17I Bsu36I EagI EclXI Eco47III
Eco52I Eco81I EcoNI FseI FspI
KspI MluI NcoI NheI NotI
NruI PacI PmaCI PmeI PmlI
Psp1406I PvuI SacII SalI SbfI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 2417 GACGTC GACGT^C
Acc65I 6437 GGTACC G^GTACC
AgeI 1514 ACCGGT A^CCGGT
AhdI 3331 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1658 GGGCCC GGGCC^C
Asp718I 6437 GGTACC G^GTACC
AspEI 3331 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3439 GGATCC G^GATCC
BbeI 4135 GGCGCC GGCGC^C
BclI 3851 TGATCA T^GATCA
BlpI 2218 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2218 GCTGAGC GC^T[ACGT]AGC
BspLU11I 1914 ACATGT A^CATGT
BsrBI 1784 CCGCTC CCGCTC(-3/-3)
BssHII 3475 GCGCGC G^CGCGC
BstEII 5141 GGTTACC G^GT[ACGT]ACC
BstPI 5141 GGTTACC G^GT[ACGT]ACC
CelII 2218 GCTGAGC GC^T[ACGT]AGC
DsaI 5315 CCACGG C^C[GA][CT]GG
Eam1105I 3331 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5141 GGTTACC G^GT[ACGT]ACC
FbaI 3851 TGATCA T^GATCA
HaeII 4135 GGCGCC [GA]GCGC^[CT]
HindIII 2905 AAGCTT A^AGCTT
HpaI 4204 GTTAAC GTT^AAC
KasI 4135 GGCGCC G^GCGCC
KpnI 6437 GGTACC GGTAC^C
NarI 4135 GGCGCC GG^CGCC
PinAI 1514 ACCGGT A^CCGGT
ScaI 7313 AGTACT AGT^ACT
SexAI 2330 ACCTGGT A^CC[AT]GGT
SfoI 4135 GGCGCC GGC^GCC
SphI 5060 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 1499 TCCGGA T^CCGGA
5265 TCCGGA
Asp700I 405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4828 GAACGTATTC
AspI 2329 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
8016 GACTCAGTC
BglII 7625 AGATCT A^GATCT
7858 AGATCT
BseAI 1499 TCCGGA T^CCGGA
5265 TCCGGA
BspEI 1499 TCCGGA T^CCGGA
5265 TCCGGA
BstBI 1127 TTCGAA TT^CGAA
6465 TTCGAA
CpoI 1516 CGGTCCG CG^G[AT]CCG
3001 CGGACCG
DraIII 118 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1636 CACGATGTG
EcoT22I 876 ATGCAT ATGCA^T
5996 ATGCAT
MroI 1499 TCCGGA T^CCGGA
5265 TCCGGA
NaeI 646 GCCGGC GCC^GGC
5068 GCCGGC
NgoMIV 646 GCCGGC G^CCGGC
5068 GCCGGC
NsiI 876 ATGCAT ATGCA^T
5996 ATGCAT
NspV 1127 TTCGAA TT^CGAA
6465 TTCGAA
PaeR7I 1754 CTCGAG C^TCGAG
5655 CTCGAG
PflFI 2329 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
8016 GACTCAGTC
PflMI 1319 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4117 CCATTTGATGG
PshAI 1247 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5168 GACTCCTGTC
RsrII 1516 CGGTCCG CG^G[AT]CCG
3001 CGGACCG
SacI 475 GAGCTC GAGCT^C
2669 GAGCTC
SfuI 1127 TTCGAA TT^CGAA
6465 TTCGAA
Tth111I 2329 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
8016 GACTCAGTC
Van91I 1319 CCAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4117 CCATTTGATGG
XhoI 1754 CTCGAG C^TCGAG
5655 CTCGAG
XmnI 405 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4828 GAACGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I ClaI DdeI DpnII
DraI DraII DrdI EaeI EarI
EcoO109I EcoRI EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XcmI XhoII