################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## Acc65I AccIII AclI AgeI Alw44I ApaI ApaLI AscI Asp700I Asp718I AviII AvrII BamHI BbrPI BciVI BlnI BsaAI BsaWI BseAI BsiEI BsiWI BspEI BspLU11I BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Cfr10I CpoI DraII DraIII DsaI EagI EclXI Eco52I EcoO109I EcoO65I EcoRV FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NaeI NcoI NgoMIV NotI NruI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacII SalI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SspI StuI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 1597 GTCTAC GT^[AC][GT]AC AflII 1075 CTTAAG C^TTAAG AflIII 2874 ACGTGT A^C[GA][CT]GT AocI 1960 CCTTAGG CC^T[ACGT]AGG Aor51HI 1986 AGCGCT AGC^GCT BalI 2436 TGGCCA TGG^CCA BcnI 511 CCCGG CC^[GC]GG BfrI 1075 CTTAAG C^TTAAG BglI 2438 GCCACAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 466 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 2880 CGTCTC CGTCTC(1/5) Bsp1407I 3349 TGTACA T^GTACA BspDI 473 ATCGAT AT^CGAT BsrGI 3349 TGTACA T^GTACA BssSI 3110 CACGAG CACGAG(-5/-1) BstBI 3201 TTCGAA TT^CGAA BstXI 1915 CCATGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1960 CCTTAGG CC^T[ACGT]AGG ClaI 473 ATCGAT AT^CGAT DrdI 2630 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1986 AGCGCT AGC^GCT Eco81I 1960 CCTTAGG CC^T[ACGT]AGG EcoRI 2941 GAATTC G^AATTC HgaI 1258 GCGTC GACGC(5/10) HindIII 1907 AAGCTT A^AGCTT MamI 466 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2436 TGGCCA TGG^CCA MscI 2436 TGGCCA TGG^CCA NciI 511 CCCGG CC^[GC]GG NdeI 3575 CATATG CA^TATG NheI 613 GCTAGC G^CTAGC NspI 1485 GCATGC [GA]CATG^[CT] NspV 3201 TTCGAA TT^CGAA PaeR7I 2316 CTCGAG C^TCGAG PflMI 934 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SbfI 2212 CCTGCAGG CCTGCA^GG SfuI 3201 TTCGAA TT^CGAA SphI 1485 GCATGC GCATG^C Sse8387I 2212 CCTGCAGG CCTGCA^GG SspBI 3349 TGTACA T^GTACA SwaI 3511 ATTTAAAT ATTT^AAAT Van91I 934 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2390 TCTAGA T^CTAGA XhoI 2316 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AatII 2838 GACGTC GACGT^C 2921 GACGTC AhdI 1517 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2873 GACGTGTCGTC AspEI 1517 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2873 GACGTGTCGTC AspI 25 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3303 GACTTGGTC BanI 456 GGCGCC G^G[CT][GA]CC 2343 GGCGCC BbeI 456 GGCGCC GGCGC^C 2343 GGCGCC BbvCI 877 CCTCAGC CCTCAGC(-5/-2) 2624 GCTGAGG BcgI 2888 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2898 GCATCAAGCTCG BclI 1455 TGATCA T^GATCA 3361 TGATCA BlpI 1909 GCTTAGC GC^T[ACGT]AGC 2645 GCTCAGC Bpu1102I 1909 GCTTAGC GC^T[ACGT]AGC 2645 GCTCAGC BsaI 734 GAGACC GGTCTC(1/5) 2319 GAGACC BseRI 587 GAGGAG GAGGAG(10/8) 2828 GAGGAG BsmI 236 GAATGC GAATGC(1/-1) 1750 GAATGC BsrBI 1389 CCGCTC CCGCTC(-3/-3) 2963 GAGCGG CelII 1909 GCTTAGC GC^T[ACGT]AGC 2645 GCTCAGC EaeI 2436 TGGCCA [CT]^GGCC[GA] 2562 TGGCCG Eam1105I 1517 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2873 GACGTGTCGTC EcoT22I 2355 ATGCAT ATGCA^T 3372 ATGCAT FauI 2719 GCGGG CCCGC(4/6) 2965 GCGGG FbaI 1455 TGATCA T^GATCA 3361 TGATCA HapII 512 CCGG C^CGG 2341 CCGG HincII 785 GTTGAC GT[CT]^[GA]AC 3501 GTCAAC HindII 785 GTTGAC GT[CT]^[GA]AC 3501 GTCAAC HpaII 512 CCGG C^CGG 2341 CCGG KasI 456 GGCGCC G^GCGCC 2343 GGCGCC MspI 512 CCGG C^CGG 2341 CCGG NarI 456 GGCGCC GG^CGCC 2343 GGCGCC NsiI 2355 ATGCAT ATGCA^T 3372 ATGCAT PflFI 25 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3303 GACTTGGTC SapI 1310 GAAGAGC GCTCTTC(1/4) 2509 GAAGAGC SfoI 456 GGCGCC GGC^GCC 2343 GGCGCC Tth111I 25 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3303 GACTTGGTC XcmI 2139 CCATTGGAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3383 CCAGGAAGATTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HhaI Hin1I HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PshBI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII