##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaI ApaLI AscI Asp700I Asp718I
AviII AvrII BamHI BbrPI BciVI
BlnI BsaAI BsaWI BseAI BsiEI
BsiWI BspEI BspLU11I BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstZ17I
Cfr10I CpoI DraII DraIII DsaI
EagI EclXI Eco52I EcoO109I EcoO65I
EcoRV FseI FspI HpaI KpnI
KspI MfeI MluI MroI MunI
NaeI NcoI NgoMIV NotI NruI
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SgrAI SmaI SnaBI SpeI SspI
StuI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1597 GTCTAC GT^[AC][GT]AC
AflII 1075 CTTAAG C^TTAAG
AflIII 2874 ACGTGT A^C[GA][CT]GT
AocI 1960 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1986 AGCGCT AGC^GCT
BalI 2436 TGGCCA TGG^CCA
BcnI 511 CCCGG CC^[GC]GG
BfrI 1075 CTTAAG C^TTAAG
BglI 2438 GCCACAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 466 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2880 CGTCTC CGTCTC(1/5)
Bsp1407I 3349 TGTACA T^GTACA
BspDI 473 ATCGAT AT^CGAT
BsrGI 3349 TGTACA T^GTACA
BssSI 3110 CACGAG CACGAG(-5/-1)
BstBI 3201 TTCGAA TT^CGAA
BstXI 1915 CCATGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1960 CCTTAGG CC^T[ACGT]AGG
ClaI 473 ATCGAT AT^CGAT
DrdI 2630 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1986 AGCGCT AGC^GCT
Eco81I 1960 CCTTAGG CC^T[ACGT]AGG
EcoRI 2941 GAATTC G^AATTC
HgaI 1258 GCGTC GACGC(5/10)
HindIII 1907 AAGCTT A^AGCTT
MamI 466 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2436 TGGCCA TGG^CCA
MscI 2436 TGGCCA TGG^CCA
NciI 511 CCCGG CC^[GC]GG
NdeI 3575 CATATG CA^TATG
NheI 613 GCTAGC G^CTAGC
NspI 1485 GCATGC [GA]CATG^[CT]
NspV 3201 TTCGAA TT^CGAA
PaeR7I 2316 CTCGAG C^TCGAG
PflMI 934 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 2212 CCTGCAGG CCTGCA^GG
SfuI 3201 TTCGAA TT^CGAA
SphI 1485 GCATGC GCATG^C
Sse8387I 2212 CCTGCAGG CCTGCA^GG
SspBI 3349 TGTACA T^GTACA
SwaI 3511 ATTTAAAT ATTT^AAAT
Van91I 934 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2390 TCTAGA T^CTAGA
XhoI 2316 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 2838 GACGTC GACGT^C
2921 GACGTC
AhdI 1517 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2873 GACGTGTCGTC
AspEI 1517 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2873 GACGTGTCGTC
AspI 25 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3303 GACTTGGTC
BanI 456 GGCGCC G^G[CT][GA]CC
2343 GGCGCC
BbeI 456 GGCGCC GGCGC^C
2343 GGCGCC
BbvCI 877 CCTCAGC CCTCAGC(-5/-2)
2624 GCTGAGG
BcgI 2888 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2898 GCATCAAGCTCG
BclI 1455 TGATCA T^GATCA
3361 TGATCA
BlpI 1909 GCTTAGC GC^T[ACGT]AGC
2645 GCTCAGC
Bpu1102I 1909 GCTTAGC GC^T[ACGT]AGC
2645 GCTCAGC
BsaI 734 GAGACC GGTCTC(1/5)
2319 GAGACC
BseRI 587 GAGGAG GAGGAG(10/8)
2828 GAGGAG
BsmI 236 GAATGC GAATGC(1/-1)
1750 GAATGC
BsrBI 1389 CCGCTC CCGCTC(-3/-3)
2963 GAGCGG
CelII 1909 GCTTAGC GC^T[ACGT]AGC
2645 GCTCAGC
EaeI 2436 TGGCCA [CT]^GGCC[GA]
2562 TGGCCG
Eam1105I 1517 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2873 GACGTGTCGTC
EcoT22I 2355 ATGCAT ATGCA^T
3372 ATGCAT
FauI 2719 GCGGG CCCGC(4/6)
2965 GCGGG
FbaI 1455 TGATCA T^GATCA
3361 TGATCA
HapII 512 CCGG C^CGG
2341 CCGG
HincII 785 GTTGAC GT[CT]^[GA]AC
3501 GTCAAC
HindII 785 GTTGAC GT[CT]^[GA]AC
3501 GTCAAC
HpaII 512 CCGG C^CGG
2341 CCGG
KasI 456 GGCGCC G^GCGCC
2343 GGCGCC
MspI 512 CCGG C^CGG
2341 CCGG
NarI 456 GGCGCC GG^CGCC
2343 GGCGCC
NsiI 2355 ATGCAT ATGCA^T
3372 ATGCAT
PflFI 25 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3303 GACTTGGTC
SapI 1310 GAAGAGC GCTCTTC(1/4)
2509 GAAGAGC
SfoI 456 GGCGCC GGC^GCC
2343 GGCGCC
Tth111I 25 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3303 GACTTGGTC
XcmI 2139 CCATTGGAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3383 CCAGGAAGATTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaI AvaII BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoNI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI Hin1I HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PshBI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII