##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AclI Aor51HI AscI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BglI BlnI BsaAI BsiEI BsiWI
BsoBI BspDI BssHII BssSI BstBI
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoRV FauI
FseI FspI KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1784 GACGTC GACGT^C
Acc65I 1318 GGTACC G^GTACC
AccII 2787 CGCG CG^CG
AccIII 4126 TCCGGA T^CCGGA
Alw44I 6179 GTGCAC G^TGCAC
ApaLI 6179 GTGCAC G^TGCAC
AseI 7995 ATTAAT AT^TAAT
AsnI 7995 ATTAAT AT^TAAT
Asp718I 1318 GGTACC G^GTACC
BalI 1467 TGGCCA TGG^CCA
BbvCI 3647 GCTGAGG CCTCAGC(-5/-2)
BcnI 191 CCCGG CC^[GC]GG
BseAI 4126 TCCGGA T^CCGGA
BsgI 1557 CTGCAC GTGCAG(16/14)
BspEI 4126 TCCGGA T^CCGGA
BsrBI 2219 GAGCGG CCGCTC(-3/-3)
Bst1107I 3252 GTATAC GTA^TAC
BstUI 2787 CGCG CG^CG
BstZ17I 3252 GTATAC GTA^TAC
DsaI 3798 CCATGG C^C[GA][CT]GG
EaeI 1467 TGGCCA [CT]^GGCC[GA]
EcoRI 3555 GAATTC G^AATTC
HindIII 6030 AAGCTT A^AGCTT
HpaI 2605 GTTAAC GTT^AAC
KpnI 1318 GGTACC GGTAC^C
MluNI 1467 TGGCCA TGG^CCA
MroI 4126 TCCGGA T^CCGGA
MscI 1467 TGGCCA TGG^CCA
MvnI 2787 CGCG CG^CG
NciI 191 CCCGG CC^[GC]GG
NcoI 3798 CCATGG C^CATGG
NdeI 3842 CATATG CA^TATG
PshBI 7995 ATTAAT AT^TAAT
SexAI 3103 ACCAGGT A^CC[AT]GGT
StuI 5507 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1784 GACGTC G[GA]^CG[CT]C
2175 GACGCC
AflII 2239 CTTAAG C^TTAAG
2959 CTTAAG
AgeI 4787 ACCGGT A^CCGGT
7798 ACCGGT
AhdI 1100 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6810 GACAAATTGTC
AspEI 1100 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6810 GACAAATTGTC
BcgI 4929 CGAGCTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1724 GCAGTGGAGTCG
BciVI 966 GGATAC GTATCC(6/5)
3886 GTATCC
BfrI 2239 CTTAAG C^TTAAG
2959 CTTAAG
BglII 6492 AGATCT A^GATCT
7446 AGATCT
BsaBI 1249 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5517 GATTTTTATC
BsaHI 1784 GACGTC G[GA]^CG[CT]C
2175 GACGCC
BsmBI 1786 CGTCTC CGTCTC(1/5)
4209 GAGACG
Bsp1407I 547 TGTACA T^GTACA
2543 TGTACA
BsrFI 4787 ACCGGT [GA]^CCGG[CT]
7798 ACCGGT
BsrGI 547 TGTACA T^GTACA
2543 TGTACA
Cfr10I 4787 ACCGGT [GA]^CCGG[CT]
7798 ACCGGT
DraIII 69 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2372 CACTGTGTG
Eam1105I 1100 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6810 GACAAATTGTC
EcoT22I 4161 ATGCAT ATGCA^T
7479 ATGCAT
HaeII 1658 GGCGCT [GA]GCGC^[CT]
5586 AGCGCC
Hin1I 1784 GACGTC G[GA]^CG[CT]C
2175 GACGCC
MamI 1249 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5517 GATTTTTATC
MfeI 1206 CAATTG C^AATTG
5387 CAATTG
MunI 1206 CAATTG C^AATTG
5387 CAATTG
NsiI 4161 ATGCAT ATGCA^T
7479 ATGCAT
PinAI 4787 ACCGGT A^CCGGT
7798 ACCGGT
SacI 229 GAGCTC GAGCT^C
4930 GAGCTC
SalI 318 GTCGAC G^TCGAC
2718 GTCGAC
SphI 1706 GCATGC GCATG^C
4159 GCATGC
SspBI 547 TGTACA T^GTACA
2543 TGTACA
XcmI 1590 CCAGGGGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3594 CCAAGTCCGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BclI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshAI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI