##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
AscI AseI AsnI AspEI BamHI
BbeI BbrPI BcgI BsaAI BsaHI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BssHII BstBI BstEII
BstPI ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I FseI HgaI
Hin1I KasI MluI MroI NarI
NotI NruI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 7815 GGTACC G^GTACC
AflIII 561 ACATGT A^C[GA][CT]GT
AgeI 922 ACCGGT A^CCGGT
Alw44I 1295 GTGCAC G^TGCAC
ApaLI 1295 GTGCAC G^TGCAC
Asp718I 7815 GGTACC G^GTACC
AviII 6043 TGCGCA TGC^GCA
BglII 1988 AGATCT A^GATCT
BsaBI 7538 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 561 ACATGT A^CATGT
BssSI 5111 CTCGTG CACGAG(-5/-1)
Bst1107I 6586 GTATAC GTA^TAC
BstAPI 1288 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 6586 GTATAC GTA^TAC
DrdI 4085 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 5348 GAATTC G^AATTC
FspI 6043 TGCGCA TGC^GCA
HpaI 6982 GTTAAC GTT^AAC
KpnI 7815 GGTACC GGTAC^C
KspI 5033 CCGCGG CCGC^GG
MamI 7538 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2972 GCCGGC GCC^GGC
NgoMIV 2972 GCCGGC G^CCGGC
PinAI 922 ACCGGT A^CCGGT
SacII 5033 CCGCGG CCGC^GG
SfiI 1718 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 3934 TCGA T^CGA
TthHB8I 3934 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 3965 CGCG CG^CG
5034 CGCG
Aor51HI 6594 AGCGCT AGC^GCT
6651 AGCGCT
Asp700I 3936 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4843 GAATACATTC
AvrII 4792 CCTAGG C^CTAGG
6788 CCTAGG
BbvCI 2002 CCTCAGC CCTCAGC(-5/-2)
2816 CCTCAGC
BlnI 4792 CCTAGG C^CTAGG
6788 CCTAGG
BsrDI 461 CATTGC GCAATG(2/0)
2856 GCAATG
BstUI 3965 CGCG CG^CG
5034 CGCG
BstYI 1988 AGATCT [GA]^GATC[CT]
5679 GGATCT
DraI 5931 TTTAAA TTT^AAA
6772 TTTAAA
Eco47III 6594 AGCGCT AGC^GCT
6651 AGCGCT
EcoRV 5096 GATATC GAT^ATC
6904 GATATC
HindIII 4609 AAGCTT A^AGCTT
5897 AAGCTT
MflI 1988 AGATCT [GA]^GATC[CT]
5679 GGATCT
MvnI 3965 CGCG CG^CG
5034 CGCG
NdeI 7547 CATATG CA^TATG
7790 CATATG
SmaI 1722 CCCGGG CCC^GGG
4128 CCCGGG
SpeI 422 ACTAGT A^CTAGT
2936 ACTAGT
XbaI 878 TCTAGA T^CTAGA
5682 TCTAGA
XhoII 1988 AGATCT [GA]^GATC[CT]
5679 GGATCT
XmaCI 1722 CCCGGG C^CCGGG
4128 CCCGGG
XmaI 1722 CCCGGG C^CCGGG
4128 CCCGGG
XmnI 3936 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4843 GAATACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BfrI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstXI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NcoI NdeII NheI NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XcmI