################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AclI AcyI AhdI AscI AseI AsnI AspEI BamHI BbeI BbrPI BcgI BsaAI BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BssHII BstBI BstEII BstPI ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I FseI HgaI Hin1I KasI MluI MroI NarI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 7815 GGTACC G^GTACC AflIII 561 ACATGT A^C[GA][CT]GT AgeI 922 ACCGGT A^CCGGT Alw44I 1295 GTGCAC G^TGCAC ApaLI 1295 GTGCAC G^TGCAC Asp718I 7815 GGTACC G^GTACC AviII 6043 TGCGCA TGC^GCA BglII 1988 AGATCT A^GATCT BsaBI 7538 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 561 ACATGT A^CATGT BssSI 5111 CTCGTG CACGAG(-5/-1) Bst1107I 6586 GTATAC GTA^TAC BstAPI 1288 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 6586 GTATAC GTA^TAC DrdI 4085 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 5348 GAATTC G^AATTC FspI 6043 TGCGCA TGC^GCA HpaI 6982 GTTAAC GTT^AAC KpnI 7815 GGTACC GGTAC^C KspI 5033 CCGCGG CCGC^GG MamI 7538 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2972 GCCGGC GCC^GGC NgoMIV 2972 GCCGGC G^CCGGC PinAI 922 ACCGGT A^CCGGT SacII 5033 CCGCGG CCGC^GG SfiI 1718 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 3934 TCGA T^CGA TthHB8I 3934 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 3965 CGCG CG^CG 5034 CGCG Aor51HI 6594 AGCGCT AGC^GCT 6651 AGCGCT Asp700I 3936 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4843 GAATACATTC AvrII 4792 CCTAGG C^CTAGG 6788 CCTAGG BbvCI 2002 CCTCAGC CCTCAGC(-5/-2) 2816 CCTCAGC BlnI 4792 CCTAGG C^CTAGG 6788 CCTAGG BsrDI 461 CATTGC GCAATG(2/0) 2856 GCAATG BstUI 3965 CGCG CG^CG 5034 CGCG BstYI 1988 AGATCT [GA]^GATC[CT] 5679 GGATCT DraI 5931 TTTAAA TTT^AAA 6772 TTTAAA Eco47III 6594 AGCGCT AGC^GCT 6651 AGCGCT EcoRV 5096 GATATC GAT^ATC 6904 GATATC HindIII 4609 AAGCTT A^AGCTT 5897 AAGCTT MflI 1988 AGATCT [GA]^GATC[CT] 5679 GGATCT MvnI 3965 CGCG CG^CG 5034 CGCG NdeI 7547 CATATG CA^TATG 7790 CATATG SmaI 1722 CCCGGG CCC^GGG 4128 CCCGGG SpeI 422 ACTAGT A^CTAGT 2936 ACTAGT XbaI 878 TCTAGA T^CTAGA 5682 TCTAGA XhoII 1988 AGATCT [GA]^GATC[CT] 5679 GGATCT XmaCI 1722 CCCGGG C^CCGGG 4128 CCCGGG XmaI 1722 CCCGGG C^CCGGG 4128 CCCGGG XmnI 3936 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4843 GAATACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AluI AlwI AlwNI AocI ApaI ApoI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BfrI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NsiI NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I Van91I XcmI