################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AclI AflIII AscI AseI AsnI AviII BciVI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BstBI ClaI CpoI EagI EclXI Eco52I EcoRV FseI FspI HpaI MamI MluI NdeI NotI NruI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SfiI SfuI SgrAI SpeI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 1976 GACGTC GACGT^C Acc65I 6736 GGTACC G^GTACC AccIII 1515 TCCGGA T^CCGGA AgeI 5540 ACCGGT A^CCGGT AhdI 1250 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3278 AGCGCT AGC^GCT Asp718I 6736 GGTACC G^GTACC AspEI 1250 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 51 GGCGCC GGCGC^C BcgI 924 GCAATGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 838 TGATCA T^GATCA BseAI 1515 TCCGGA T^CCGGA BspEI 1515 TCCGGA T^CCGGA BsrBI 1095 CCGCTC CCGCTC(-3/-3) BssSI 3125 CACGAG CACGAG(-5/-1) Bst1107I 5508 GTATAC GTA^TAC BstAPI 138 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 7838 GGTGACC G^GT[ACGT]ACC BstPI 7838 GGTGACC G^GT[ACGT]ACC BstZ17I 5508 GTATAC GTA^TAC DrdI 4178 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1250 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3278 AGCGCT AGC^GCT EcoO65I 7838 GGTGACC G^GT[ACGT]ACC EcoRI 6457 GAATTC G^AATTC FbaI 838 TGATCA T^GATCA KasI 51 GGCGCC G^GCGCC KpnI 6736 GGTACC GGTAC^C MroI 1515 TCCGGA T^CCGGA NarI 51 GGCGCC GG^CGCC PinAI 5540 ACCGGT A^CCGGT PshAI 731 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 2809 CCTGCAGG CCTGCA^GG SfoI 51 GGCGCC GGC^GCC SnaBI 4727 TACGTA TAC^GTA Sse8387I 2809 CCTGCAGG CCTGCA^GG SspI 7953 AATATT AAT^ATT XbaI 1897 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvrII 4384 CCTAGG C^CTAGG 4589 CCTAGG BlnI 4384 CCTAGG C^CTAGG 4589 CCTAGG BsiEI 3132 CGGTCG CG[GA][CT]^CG 3574 CGACCG BstXI 4242 CCATCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 7843 CCAGGGCCTTGG EcoT22I 2870 ATGCAT ATGCA^T 7385 ATGCAT KspI 37 CCGCGG CCGC^GG 154 CCGCGG MfeI 5407 CAATTG C^AATTG 7828 CAATTG MunI 5407 CAATTG C^AATTG 7828 CAATTG NaeI 1581 GCCGGC GCC^GGC 4613 GCCGGC NgoMIV 1581 GCCGGC G^CCGGC 4613 GCCGGC NheI 4661 GCTAGC G^CTAGC 7742 GCTAGC NsiI 2870 ATGCAT ATGCA^T 7385 ATGCAT SacII 37 CCGCGG CCGC^GG 154 CCGCGG ScaI 2721 AGTACT AGT^ACT 5391 AGTACT SexAI 1665 ACCAGGT A^CC[AT]GGT 6123 ACCAGGT SmaI 232 CCCGGG CCC^GGG 6871 CCCGGG XmaCI 232 CCCGGG C^CCGGG 6871 CCCGGG XmaI 232 CCCGGG C^CCGGG 6871 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 155 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflII AluI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI Asp700I AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI