##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AclI AflIII AscI AseI AsnI
AviII BciVI BsaBI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BstBI ClaI CpoI EagI EclXI
Eco52I EcoRV FseI FspI HpaI
MamI MluI NdeI NotI NruI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfiI SfuI SgrAI SpeI SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1976 GACGTC GACGT^C
Acc65I 6736 GGTACC G^GTACC
AccIII 1515 TCCGGA T^CCGGA
AgeI 5540 ACCGGT A^CCGGT
AhdI 1250 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3278 AGCGCT AGC^GCT
Asp718I 6736 GGTACC G^GTACC
AspEI 1250 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 51 GGCGCC GGCGC^C
BcgI 924 GCAATGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 838 TGATCA T^GATCA
BseAI 1515 TCCGGA T^CCGGA
BspEI 1515 TCCGGA T^CCGGA
BsrBI 1095 CCGCTC CCGCTC(-3/-3)
BssSI 3125 CACGAG CACGAG(-5/-1)
Bst1107I 5508 GTATAC GTA^TAC
BstAPI 138 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 7838 GGTGACC G^GT[ACGT]ACC
BstPI 7838 GGTGACC G^GT[ACGT]ACC
BstZ17I 5508 GTATAC GTA^TAC
DrdI 4178 GACTGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1250 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3278 AGCGCT AGC^GCT
EcoO65I 7838 GGTGACC G^GT[ACGT]ACC
EcoRI 6457 GAATTC G^AATTC
FbaI 838 TGATCA T^GATCA
KasI 51 GGCGCC G^GCGCC
KpnI 6736 GGTACC GGTAC^C
MroI 1515 TCCGGA T^CCGGA
NarI 51 GGCGCC GG^CGCC
PinAI 5540 ACCGGT A^CCGGT
PshAI 731 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2809 CCTGCAGG CCTGCA^GG
SfoI 51 GGCGCC GGC^GCC
SnaBI 4727 TACGTA TAC^GTA
Sse8387I 2809 CCTGCAGG CCTGCA^GG
SspI 7953 AATATT AAT^ATT
XbaI 1897 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvrII 4384 CCTAGG C^CTAGG
4589 CCTAGG
BlnI 4384 CCTAGG C^CTAGG
4589 CCTAGG
BsiEI 3132 CGGTCG CG[GA][CT]^CG
3574 CGACCG
BstXI 4242 CCATCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7843 CCAGGGCCTTGG
EcoT22I 2870 ATGCAT ATGCA^T
7385 ATGCAT
KspI 37 CCGCGG CCGC^GG
154 CCGCGG
MfeI 5407 CAATTG C^AATTG
7828 CAATTG
MunI 5407 CAATTG C^AATTG
7828 CAATTG
NaeI 1581 GCCGGC GCC^GGC
4613 GCCGGC
NgoMIV 1581 GCCGGC G^CCGGC
4613 GCCGGC
NheI 4661 GCTAGC G^CTAGC
7742 GCTAGC
NsiI 2870 ATGCAT ATGCA^T
7385 ATGCAT
SacII 37 CCGCGG CCGC^GG
154 CCGCGG
ScaI 2721 AGTACT AGT^ACT
5391 AGTACT
SexAI 1665 ACCAGGT A^CC[AT]GGT
6123 ACCAGGT
SmaI 232 CCCGGG CCC^GGG
6871 CCCGGG
XmaCI 232 CCCGGG C^CCGGG
6871 CCCGGG
XmaI 232 CCCGGG C^CCGGG
6871 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp700I AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BfrI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI