################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AclI AflII Alw44I ApaI ApaLI AscI Asp700I Asp718I AviII AvrII BamHI BbvCI BcgI BclI BfrI BlnI BsaBI BsiWI BspDI BssHII Bst1107I BstAPI BstXI BstZ17I ClaI CpoI DraIII DrdI FbaI FseI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PmeI PshAI Psp1406I PvuI RsrII SacII SalI SapI SexAI SfiI SgrAI SmlI SnaBI SphI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccIII 1413 TCCGGA T^CCGGA AgeI 80 ACCGGT A^CCGGT AhdI 683 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1877 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1696 AGCGCT AGC^GCT AseI 1310 ATTAAT AT^TAAT AsnI 1310 ATTAAT AT^TAAT AspEI 683 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2387 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 272 GGCGCC GGCGC^C BbrPI 161 CACGTG CAC^GTG BciVI 745 GGATAC GTATCC(6/5) BsaAI 161 CACGTG [CT]AC^GT[GA] BseAI 1413 TCCGGA T^CCGGA BseRI 997 GAGGAG GAGGAG(10/8) BsiEI 1465 CGGCCG CG[GA][CT]^CG BsmBI 89 CGTCTC CGTCTC(1/5) Bsp1407I 1278 TGTACA T^GTACA BspEI 1413 TCCGGA T^CCGGA BspLU11I 1057 ACATGT A^CATGT BsrBI 58 GAGCGG CCGCTC(-3/-3) BsrGI 1278 TGTACA T^GTACA BssSI 18 CACGAG CACGAG(-5/-1) BstBI 1206 TTCGAA TT^CGAA BstEII 2933 GGTCACC G^GT[ACGT]ACC BstPI 2933 GGTCACC G^GT[ACGT]ACC DraI 3419 TTTAAA TTT^AAA EagI 1465 CGGCCG C^GGCCG Eam1105I 683 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1465 CGGCCG C^GGCCG Eco47III 1696 AGCGCT AGC^GCT Eco52I 1465 CGGCCG C^GGCCG EcoO65I 2933 GGTCACC G^GT[ACGT]ACC EcoRI 1586 GAATTC G^AATTC EcoRV 2060 GATATC GAT^ATC EcoT22I 1404 ATGCAT ATGCA^T HindIII 2309 AAGCTT A^AGCTT KasI 272 GGCGCC G^GCGCC MroI 1413 TCCGGA T^CCGGA NarI 272 GGCGCC GG^CGCC NcoI 1329 CCATGG C^CATGG NdeI 2096 CATATG CA^TATG NsiI 1404 ATGCAT ATGCA^T NspV 1206 TTCGAA TT^CGAA PflFI 2387 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 258 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 80 ACCGGT A^CCGGT PmaCI 161 CACGTG CAC^GTG PmlI 161 CACGTG CAC^GTG PshBI 1310 ATTAAT AT^TAAT PvuII 1034 CAGCTG CAG^CTG SacI 3211 GAGCTC GAGCT^C SbfI 2035 CCTGCAGG CCTGCA^GG ScaI 2208 AGTACT AGT^ACT SfoI 272 GGCGCC GGC^GCC SfuI 1206 TTCGAA TT^CGAA SpeI 2676 ACTAGT A^CTAGT Sse8387I 2035 CCTGCAGG CCTGCA^GG SspBI 1278 TGTACA T^GTACA Tth111I 2387 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 258 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 84 GTCTAC GT^[AC][GT]AC 3063 GTCTAC AccII 47 CGCG CG^CG 177 CGCG AcyI 132 GGCGTC G[GA]^CG[CT]C 272 GGCGCC AocI 2858 CCTAAGG CC^T[ACGT]AGG 3289 CCTAAGG AspHI 1068 GAGCAC G[AT]GC[AT]^C 3211 GAGCTC BglI 1458 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3286 GCCCCTAAGGC BglII 1574 AGATCT A^GATCT 2447 AGATCT BsaHI 132 GGCGTC G[GA]^CG[CT]C 272 GGCGCC BsaWI 80 ACCGGT [AT]^CCGG[AT] 1413 TCCGGA BsgI 2416 CTGCAC GTGCAG(16/14) 3117 CTGCAC BsiHKAI 1068 GAGCAC G[AT]GC[AT]^C 3211 GAGCTC BspHI 862 TCATGA T^CATGA 2658 TCATGA BstUI 47 CGCG CG^CG 177 CGCG Bsu36I 2858 CCTAAGG CC^T[ACGT]AGG 3289 CCTAAGG DraII 183 AGGACCT [GA]G^G[ACGT]CC[CT] 1901 GGGACCT DsaI 727 CCACGG C^C[GA][CT]GG 1329 CCATGG Eco81I 2858 CCTAAGG CC^T[ACGT]AGG 3289 CCTAAGG EcoNI 1905 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2793 CCTGGAGTAGG EcoO109I 183 AGGACCT [GA]G^G[ACGT]CC[CT] 1901 GGGACCT FauI 3 GCGGG CCCGC(4/6) 1075 GCGGG Hin1I 132 GGCGTC G[GA]^CG[CT]C 272 GGCGCC MvnI 47 CGCG CG^CG 177 CGCG PpuMI 183 AGGACCT [GA]G^G[AT]CC[CT] 1901 GGGACCT RcaI 862 TCATGA T^CATGA 2658 TCATGA SmaI 145 CCCGGG CCC^GGG 558 CCCGGG SspI 366 AATATT AAT^ATT 1016 AATATT StuI 2909 AGGCCT AGG^CCT 3188 AGGCCT TfiI 418 GAATC G^A[AT]TC 2030 GATTC XmaCI 145 CCCGGG C^CCGGG 558 CCCGGG XmaI 145 CCCGGG C^CCGGG 558 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII