##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AflII Alw44I
ApaI ApaLI AscI Asp700I Asp718I
AviII AvrII BamHI BbvCI BcgI
BclI BfrI BlnI BsaBI BsiWI
BspDI BssHII Bst1107I BstAPI BstXI
BstZ17I ClaI CpoI DraIII DrdI
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SexAI SfiI SgrAI
SmlI SnaBI SphI SwaI XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccIII 1413 TCCGGA T^CCGGA
AgeI 80 ACCGGT A^CCGGT
AhdI 683 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1877 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1696 AGCGCT AGC^GCT
AseI 1310 ATTAAT AT^TAAT
AsnI 1310 ATTAAT AT^TAAT
AspEI 683 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2387 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 272 GGCGCC GGCGC^C
BbrPI 161 CACGTG CAC^GTG
BciVI 745 GGATAC GTATCC(6/5)
BsaAI 161 CACGTG [CT]AC^GT[GA]
BseAI 1413 TCCGGA T^CCGGA
BseRI 997 GAGGAG GAGGAG(10/8)
BsiEI 1465 CGGCCG CG[GA][CT]^CG
BsmBI 89 CGTCTC CGTCTC(1/5)
Bsp1407I 1278 TGTACA T^GTACA
BspEI 1413 TCCGGA T^CCGGA
BspLU11I 1057 ACATGT A^CATGT
BsrBI 58 GAGCGG CCGCTC(-3/-3)
BsrGI 1278 TGTACA T^GTACA
BssSI 18 CACGAG CACGAG(-5/-1)
BstBI 1206 TTCGAA TT^CGAA
BstEII 2933 GGTCACC G^GT[ACGT]ACC
BstPI 2933 GGTCACC G^GT[ACGT]ACC
DraI 3419 TTTAAA TTT^AAA
EagI 1465 CGGCCG C^GGCCG
Eam1105I 683 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1465 CGGCCG C^GGCCG
Eco47III 1696 AGCGCT AGC^GCT
Eco52I 1465 CGGCCG C^GGCCG
EcoO65I 2933 GGTCACC G^GT[ACGT]ACC
EcoRI 1586 GAATTC G^AATTC
EcoRV 2060 GATATC GAT^ATC
EcoT22I 1404 ATGCAT ATGCA^T
HindIII 2309 AAGCTT A^AGCTT
KasI 272 GGCGCC G^GCGCC
MroI 1413 TCCGGA T^CCGGA
NarI 272 GGCGCC GG^CGCC
NcoI 1329 CCATGG C^CATGG
NdeI 2096 CATATG CA^TATG
NsiI 1404 ATGCAT ATGCA^T
NspV 1206 TTCGAA TT^CGAA
PflFI 2387 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 258 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 80 ACCGGT A^CCGGT
PmaCI 161 CACGTG CAC^GTG
PmlI 161 CACGTG CAC^GTG
PshBI 1310 ATTAAT AT^TAAT
PvuII 1034 CAGCTG CAG^CTG
SacI 3211 GAGCTC GAGCT^C
SbfI 2035 CCTGCAGG CCTGCA^GG
ScaI 2208 AGTACT AGT^ACT
SfoI 272 GGCGCC GGC^GCC
SfuI 1206 TTCGAA TT^CGAA
SpeI 2676 ACTAGT A^CTAGT
Sse8387I 2035 CCTGCAGG CCTGCA^GG
SspBI 1278 TGTACA T^GTACA
Tth111I 2387 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 258 CCATAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 84 GTCTAC GT^[AC][GT]AC
3063 GTCTAC
AccII 47 CGCG CG^CG
177 CGCG
AcyI 132 GGCGTC G[GA]^CG[CT]C
272 GGCGCC
AocI 2858 CCTAAGG CC^T[ACGT]AGG
3289 CCTAAGG
AspHI 1068 GAGCAC G[AT]GC[AT]^C
3211 GAGCTC
BglI 1458 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3286 GCCCCTAAGGC
BglII 1574 AGATCT A^GATCT
2447 AGATCT
BsaHI 132 GGCGTC G[GA]^CG[CT]C
272 GGCGCC
BsaWI 80 ACCGGT [AT]^CCGG[AT]
1413 TCCGGA
BsgI 2416 CTGCAC GTGCAG(16/14)
3117 CTGCAC
BsiHKAI 1068 GAGCAC G[AT]GC[AT]^C
3211 GAGCTC
BspHI 862 TCATGA T^CATGA
2658 TCATGA
BstUI 47 CGCG CG^CG
177 CGCG
Bsu36I 2858 CCTAAGG CC^T[ACGT]AGG
3289 CCTAAGG
DraII 183 AGGACCT [GA]G^G[ACGT]CC[CT]
1901 GGGACCT
DsaI 727 CCACGG C^C[GA][CT]GG
1329 CCATGG
Eco81I 2858 CCTAAGG CC^T[ACGT]AGG
3289 CCTAAGG
EcoNI 1905 CCTGCTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2793 CCTGGAGTAGG
EcoO109I 183 AGGACCT [GA]G^G[ACGT]CC[CT]
1901 GGGACCT
FauI 3 GCGGG CCCGC(4/6)
1075 GCGGG
Hin1I 132 GGCGTC G[GA]^CG[CT]C
272 GGCGCC
MvnI 47 CGCG CG^CG
177 CGCG
PpuMI 183 AGGACCT [GA]G^G[AT]CC[CT]
1901 GGGACCT
RcaI 862 TCATGA T^CATGA
2658 TCATGA
SmaI 145 CCCGGG CCC^GGG
558 CCCGGG
SspI 366 AATATT AAT^ATT
1016 AATATT
StuI 2909 AGGCCT AGG^CCT
3188 AGGCCT
TfiI 418 GAATC G^A[AT]TC
2030 GATTC
XmaCI 145 CCCGGG C^CCGGG
558 CCCGGG
XmaI 145 CCCGGG C^CCGGG
558 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII