##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AgeI Alw44I ApaLI AscI
Asp718I AspI AvrII BbrPI BcgI
BlnI BsiWI BsmBI BspDI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EcoO65I FseI KpnI MluI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PvuI RsrII SalI SexAI SfiI
SfuI SgrAI SnaBI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 666 GACGTC GACGT^C
AccI 7850 GTCTAC GT^[AC][GT]AC
AccIII 6207 TCCGGA T^CCGGA
AclI 1498 AACGTT AA^CGTT
AflII 1757 CTTAAG C^TTAAG
AhdI 762 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 6739 AGCGCT AGC^GCT
AseI 3330 ATTAAT AT^TAAT
AsnI 3330 ATTAAT AT^TAAT
AspEI 762 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 6378 TGCGCA TGC^GCA
BamHI 2968 GGATCC G^GATCC
BbeI 3473 GGCGCC GGCGC^C
BciVI 3306 GTATCC GTATCC(6/5)
BclI 7729 TGATCA T^GATCA
BfrI 1757 CTTAAG C^TTAAG
BsaAI 411 TACGTG [CT]AC^GT[GA]
BseAI 6207 TCCGGA T^CCGGA
BsmI 2007 GAATGC GAATGC(1/-1)
Bsp1407I 2035 TGTACA T^GTACA
BspEI 6207 TCCGGA T^CCGGA
BsrGI 2035 TGTACA T^GTACA
DrdI 5903 GACCACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 762 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6739 AGCGCT AGC^GCT
EcoT22I 715 ATGCAT ATGCA^T
FbaI 7729 TGATCA T^GATCA
FspI 6378 TGCGCA TGC^GCA
HpaI 1752 GTTAAC GTT^AAC
KasI 3473 GGCGCC G^GCGCC
MfeI 423 CAATTG C^AATTG
MroI 6207 TCCGGA T^CCGGA
MunI 423 CAATTG C^AATTG
NaeI 3579 GCCGGC GCC^GGC
NarI 3473 GGCGCC GG^CGCC
NdeI 7089 CATATG CA^TATG
NgoMIV 3579 GCCGGC G^CCGGC
NheI 2304 GCTAGC G^CTAGC
NsiI 715 ATGCAT ATGCA^T
PshBI 3330 ATTAAT AT^TAAT
Psp1406I 1498 AACGTT AA^CGTT
SbfI 3486 CCTGCAGG CCTGCA^GG
ScaI 7412 AGTACT AGT^ACT
SfoI 3473 GGCGCC GGC^GCC
SpeI 7974 ACTAGT A^CTAGT
SphI 6777 GCATGC GCATG^C
Sse8387I 3486 CCTGCAGG CCTGCA^GG
SspBI 2035 TGTACA T^GTACA
SspI 7898 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 666 GACGTC G[GA]^CG[CT]C
3473 GGCGCC
BsaBI 1295 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7025 GATCCCCATC
BsaHI 666 GACGTC G[GA]^CG[CT]C
3473 GGCGCC
BspHI 2354 TCATGA T^CATGA
5517 TCATGA
BstAPI 1995 GCACTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4528 GCAGAGCCTGC
DraIII 676 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
3917 CACGGGGTG
EagI 3656 CGGCCG C^GGCCG
6859 CGGCCG
EclXI 3656 CGGCCG C^GGCCG
6859 CGGCCG
Eco52I 3656 CGGCCG C^GGCCG
6859 CGGCCG
EcoRV 165 GATATC GAT^ATC
1299 GATATC
HgaI 56 GCGTC GACGC(5/10)
4890 GCGTC
Hin1I 666 GACGTC G[GA]^CG[CT]C
3473 GGCGCC
KspI 63 CCGCGG CCGC^GG
4067 CCGCGG
MamI 1295 GATGGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7025 GATCCCCATC
RcaI 2354 TCATGA T^CATGA
5517 TCATGA
SacII 63 CCGCGG CCGC^GG
4067 CCGCGG
SapI 4276 GAAGAGC GCTCTTC(1/4)
6610 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII XmaCI XmaI
XmnI