################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccII AclI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AspI AviII BamHI BbeI BbsI BcgI BpuAI BsaBI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrFI BssHII BssSI BstBI BstUI Cfr10I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoRV FseI FspI HgaI HincII HindII HpaI KasI KspI MamI MluI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 2455 GACGTC GACGT^C AcyI 2455 GACGTC G[GA]^CG[CT]C AflII 879 CTTAAG C^TTAAG AhdI 2425 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1243 CCTAAGG CC^T[ACGT]AGG AseI 3088 ATTAAT AT^TAAT AsnI 3088 ATTAAT AT^TAAT Asp700I 773 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2425 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 166 CACGTG CAC^GTG BciVI 648 GGATAC GTATCC(6/5) BclI 2693 TGATCA T^GATCA BfrI 879 CTTAAG C^TTAAG BglI 2728 GCCTTAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2544 AGATCT A^GATCT BlpI 805 GCTCAGC GC^T[ACGT]AGC Bpu1102I 805 GCTCAGC GC^T[ACGT]AGC BsaAI 166 CACGTG [CT]AC^GT[GA] BsaHI 2455 GACGTC G[GA]^CG[CT]C BsaI 1372 GGTCTC GGTCTC(1/5) BsmI 2091 GCATTC GAATGC(1/-1) BsrBI 1091 GAGCGG CCGCTC(-3/-3) BsrDI 2913 CATTGC GCAATG(2/0) Bst1107I 867 GTATAC GTA^TAC BstAPI 1225 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1725 GGTAACC G^GT[ACGT]ACC BstPI 1725 GGTAACC G^GT[ACGT]ACC BstZ17I 867 GTATAC GTA^TAC Bsu36I 1243 CCTAAGG CC^T[ACGT]AGG CelII 805 GCTCAGC GC^T[ACGT]AGC Eam1105I 2425 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1243 CCTAAGG CC^T[ACGT]AGG EcoO65I 1725 GGTAACC G^GT[ACGT]ACC EcoT22I 2494 ATGCAT ATGCA^T FbaI 2693 TGATCA T^GATCA Hin1I 2455 GACGTC G[GA]^CG[CT]C HindIII 143 AAGCTT A^AGCTT MfeI 1115 CAATTG C^AATTG MunI 1115 CAATTG C^AATTG NheI 380 GCTAGC G^CTAGC NsiI 2494 ATGCAT ATGCA^T NspI 3005 ACATGC [GA]CATG^[CT] PflMI 895 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 166 CACGTG CAC^GTG PmlI 166 CACGTG CAC^GTG PshBI 3088 ATTAAT AT^TAAT ScaI 1776 AGTACT AGT^ACT SmaI 1573 CCCGGG CCC^GGG Van91I 895 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1275 CCAGGAGTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1573 CCCGGG C^CCGGG XmaI 1573 CCCGGG C^CCGGG XmnI 773 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BalI 297 TGGCCA TGG^CCA 2735 TGGCCA BbvCI 615 CCTCAGC CCTCAGC(-5/-2) 2071 GCTGAGG BseRI 1352 CTCCTC GAGGAG(10/8) 2228 GAGGAG BsgI 1232 CTGCAC GTGCAG(16/14) 702 GTGCAG Bsp1407I 1339 TGTACA T^GTACA 2786 TGTACA BsrGI 1339 TGTACA T^GTACA 2786 TGTACA CfoI 378 GCGC GCG^C 2502 GCGC DrdI 673 GACACCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2118 GACTTCAGGGTC DsaI 1610 CCATGG C^C[GA][CT]GG 2063 CCATGG EcoNI 1933 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2044 CCTAGAATAGG HaeII 377 GGCGCT [GA]GCGC^[CT] 2501 AGCGCC HhaI 378 GCGC GCG^C 2502 GCGC HinP1I 378 GCGC G^CGC 2502 GCGC MluNI 297 TGGCCA TGG^CCA 2735 TGGCCA MscI 297 TGGCCA TGG^CCA 2735 TGGCCA MslI 1680 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2487 CATTCCCATG NcoI 1610 CCATGG C^CATGG 2063 CCATGG NdeI 2513 CATATG CA^TATG 3628 CATATG PvuII 1229 CAGCTG CAG^CTG 2339 CAGCTG SspBI 1339 TGTACA T^GTACA 2786 TGTACA StuI 2372 AGGCCT AGG^CCT 2670 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### Acc65I AccI AccIII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp718I AspHI AvaI AvaII AvrII BanI BanII BbvI BcnI BfaI BlnI BmyI BpmI BsaJI BsaWI BseAI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII