##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccII AclI AflIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AspI
AviII BamHI BbeI BbsI BcgI
BpuAI BsaBI BsiEI BsiWI BsmBI
BspDI BspHI BspLU11I BsrFI BssHII
BssSI BstBI BstUI Cfr10I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HgaI HincII HindII HpaI KasI
KspI MamI MluI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2455 GACGTC GACGT^C
AcyI 2455 GACGTC G[GA]^CG[CT]C
AflII 879 CTTAAG C^TTAAG
AhdI 2425 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1243 CCTAAGG CC^T[ACGT]AGG
AseI 3088 ATTAAT AT^TAAT
AsnI 3088 ATTAAT AT^TAAT
Asp700I 773 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2425 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 166 CACGTG CAC^GTG
BciVI 648 GGATAC GTATCC(6/5)
BclI 2693 TGATCA T^GATCA
BfrI 879 CTTAAG C^TTAAG
BglI 2728 GCCTTAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2544 AGATCT A^GATCT
BlpI 805 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 805 GCTCAGC GC^T[ACGT]AGC
BsaAI 166 CACGTG [CT]AC^GT[GA]
BsaHI 2455 GACGTC G[GA]^CG[CT]C
BsaI 1372 GGTCTC GGTCTC(1/5)
BsmI 2091 GCATTC GAATGC(1/-1)
BsrBI 1091 GAGCGG CCGCTC(-3/-3)
BsrDI 2913 CATTGC GCAATG(2/0)
Bst1107I 867 GTATAC GTA^TAC
BstAPI 1225 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1725 GGTAACC G^GT[ACGT]ACC
BstPI 1725 GGTAACC G^GT[ACGT]ACC
BstZ17I 867 GTATAC GTA^TAC
Bsu36I 1243 CCTAAGG CC^T[ACGT]AGG
CelII 805 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2425 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1243 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1725 GGTAACC G^GT[ACGT]ACC
EcoT22I 2494 ATGCAT ATGCA^T
FbaI 2693 TGATCA T^GATCA
Hin1I 2455 GACGTC G[GA]^CG[CT]C
HindIII 143 AAGCTT A^AGCTT
MfeI 1115 CAATTG C^AATTG
MunI 1115 CAATTG C^AATTG
NheI 380 GCTAGC G^CTAGC
NsiI 2494 ATGCAT ATGCA^T
NspI 3005 ACATGC [GA]CATG^[CT]
PflMI 895 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 166 CACGTG CAC^GTG
PmlI 166 CACGTG CAC^GTG
PshBI 3088 ATTAAT AT^TAAT
ScaI 1776 AGTACT AGT^ACT
SmaI 1573 CCCGGG CCC^GGG
Van91I 895 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1275 CCAGGAGTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1573 CCCGGG C^CCGGG
XmaI 1573 CCCGGG C^CCGGG
XmnI 773 GAAATTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BalI 297 TGGCCA TGG^CCA
2735 TGGCCA
BbvCI 615 CCTCAGC CCTCAGC(-5/-2)
2071 GCTGAGG
BseRI 1352 CTCCTC GAGGAG(10/8)
2228 GAGGAG
BsgI 1232 CTGCAC GTGCAG(16/14)
702 GTGCAG
Bsp1407I 1339 TGTACA T^GTACA
2786 TGTACA
BsrGI 1339 TGTACA T^GTACA
2786 TGTACA
CfoI 378 GCGC GCG^C
2502 GCGC
DrdI 673 GACACCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2118 GACTTCAGGGTC
DsaI 1610 CCATGG C^C[GA][CT]GG
2063 CCATGG
EcoNI 1933 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2044 CCTAGAATAGG
HaeII 377 GGCGCT [GA]GCGC^[CT]
2501 AGCGCC
HhaI 378 GCGC GCG^C
2502 GCGC
HinP1I 378 GCGC G^CGC
2502 GCGC
MluNI 297 TGGCCA TGG^CCA
2735 TGGCCA
MscI 297 TGGCCA TGG^CCA
2735 TGGCCA
MslI 1680 CATTCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2487 CATTCCCATG
NcoI 1610 CCATGG C^CATGG
2063 CCATGG
NdeI 2513 CATATG CA^TATG
3628 CATATG
PvuII 1229 CAGCTG CAG^CTG
2339 CAGCTG
SspBI 1339 TGTACA T^GTACA
2786 TGTACA
StuI 2372 AGGCCT AGG^CCT
2670 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
Acc65I AccI AccIII AciI AcsI
AfaI AluI AlwI AlwNI ApoI
Asp718I AspHI AvaI AvaII AvrII
BanI BanII BbvI BcnI BfaI
BlnI BmyI BpmI BsaJI BsaWI
BseAI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII