##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp718I AspI AviII BbeI
BbsI BcgI BfrI BpuAI BsaBI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoRV FseI FspI HaeII HpaI
KasI KpnI KspI MamI MfeI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 484 GGCGTC G[GA]^CG[CT]C
AhdI 2223 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2808 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 2223 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1771 CTCGAG C^[CT]CG[GA]G
AvrII 2310 CCTAGG C^CTAGG
BalI 2853 TGGCCA TGG^CCA
BamHI 415 GGATCC G^GATCC
BanI 1433 GGTGCC G^G[CT][GA]CC
BbrPI 210 CACGTG CAC^GTG
BciVI 1574 GGATAC GTATCC(6/5)
BclI 990 TGATCA T^GATCA
BglII 917 AGATCT A^GATCT
BlnI 2310 CCTAGG C^CTAGG
BsaAI 210 CACGTG [CT]AC^GT[GA]
BsaHI 484 GGCGTC G[GA]^CG[CT]C
BsmBI 154 GAGACG CGTCTC(1/5)
BsoBI 1771 CTCGAG C^[CT]CG[GA]G
BsrBI 199 CCGCTC CCGCTC(-3/-3)
BsrDI 1922 CATTGC GCAATG(2/0)
BstEII 2617 GGTTACC G^GT[ACGT]ACC
BstPI 2617 GGTTACC G^GT[ACGT]ACC
CfoI 3017 GCGC GCG^C
Eam1105I 2223 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2617 GGTTACC G^GT[ACGT]ACC
EcoRI 959 GAATTC G^AATTC
EcoT22I 1874 ATGCAT ATGCA^T
FbaI 990 TGATCA T^GATCA
HgaI 485 GCGTC GACGC(5/10)
HhaI 3017 GCGC GCG^C
Hin1I 484 GGCGTC G[GA]^CG[CT]C
HinP1I 3017 GCGC G^CGC
MluI 1726 ACGCGT A^CGCGT
MluNI 2853 TGGCCA TGG^CCA
MscI 2853 TGGCCA TGG^CCA
NcoI 2546 CCATGG C^CATGG
NsiI 1874 ATGCAT ATGCA^T
PaeR7I 1771 CTCGAG C^TCGAG
PmaCI 210 CACGTG CAC^GTG
PmlI 210 CACGTG CAC^GTG
XhoI 1771 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 856 CGCG CG^CG
1727 CGCG
AcsI 959 GAATTC [GA]^AATT[CT]
3437 AAATTT
Alw44I 1053 GTGCAC G^TGCAC
1332 GTGCAC
ApaLI 1053 GTGCAC G^TGCAC
1332 GTGCAC
ApoI 959 GAATTC [GA]^AATT[CT]
3437 AAATTT
Asp700I 1120 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1857 GAAATGATTC
BbvCI 68 GCTGAGG CCTCAGC(-5/-2)
1208 GCTGAGG
BcnI 1 CCCGG CC^[GC]GG
872 CCCGG
BglI 138 GCCATGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2855 GCCAAGAAGGC
BlpI 201 GCTCAGC GC^T[ACGT]AGC
223 GCTCAGC
Bpu1102I 201 GCTCAGC GC^T[ACGT]AGC
223 GCTCAGC
BsaI 165 GAGACC GGTCTC(1/5)
1460 GGTCTC
BstUI 856 CGCG CG^CG
1727 CGCG
CelII 201 GCTCAGC GC^T[ACGT]AGC
223 GCTCAGC
DrdI 1395 GACTTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2787 GACTCTTCTGTC
EaeI 196 TGGCCG [CT]^GGCC[GA]
2853 TGGCCA
HapII 2 CCGG C^CGG
873 CCGG
HincII 336 GTCAAC GT[CT]^[GA]AC
1764 GTCAAC
HindII 336 GTCAAC GT[CT]^[GA]AC
1764 GTCAAC
HpaII 2 CCGG C^CGG
873 CCGG
MspI 2 CCGG C^CGG
873 CCGG
MvnI 856 CGCG CG^CG
1727 CGCG
NciI 1 CCCGG CC^[GC]GG
872 CCCGG
NheI 577 GCTAGC G^CTAGC
2634 GCTAGC
PstI 1287 CTGCAG CTGCA^G
3037 CTGCAG
SmlI 1580 CTTGAG C^T[CT][GA]AG
1771 CTCGAG
XmnI 1120 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1857 GAAATGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AocI ApaI AspHI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII